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云南茉莉花病毒种类初步鉴定及CMV茉莉花分离物CP基因序列分析
Preliminary identification of the viruses infecting Jasmonium sambac in Yunnan Province and sequence analysis of the CP gene of CMV jasmine isolate

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张天毅 1   余代宏 2   曹玉莲 1   鲁慧 2   兰平秀 1   李梅会 2   谭冠林 3 *   李凡 1 *  
文摘 2021-2022年在云南省玉溪市和昆明市采集95份表现为花叶、黄化、褪绿、环斑嵌纹等症状的茉莉花病毒病叶片样品,利用菜豆金色花叶病毒属(Begomovirus)、黄症病毒属(Luteovirus)、马铃薯卷叶病毒属(Polerovirus)、马铃薯X病毒属(Potexvirus)、马铃薯Y病毒属(Potyvirus)、烟草花叶病毒属(Tobamovirus)、幽影病毒属(Umbravirus)等病毒属的通用引物以及黄瓜花叶病毒(cucumber mosaic virus,CMV)、茉莉花叶伴随病毒(jasmine mosaic-associated virus,JMaV)、茉莉C病毒(jasmine virus C,JaVC)、茉莉H病毒(jasmine virus H,JaVH)、茉莉T病毒(jasmine virus T,JaVT)、烟草线条病毒(tobacco streak virus,TSV)等病毒的特异性引物,对茉莉花进行上述病毒的RT-PCR检测。发现侵染云南省玉溪市元江县和昆明市呈贡区的茉莉花病毒有JaVT、JaVH、JMaV、JaVC和CMV五种,检出率分别为60.00%、57.89%、15.79%、13.68%和6.32%, JaVT和JaVH是侵染云南茉莉花的优势病毒;这5种病毒在元江县和呈贡区的茉莉花样品中均检测到,优势病毒均为JaVT和JaVH,但呈贡区的病毒检出率均低于元江县。病毒大多以复合侵染的形式对茉莉花产生危害,5种病毒共有11种复合侵染类型,JaVC和CMV通常与JaVH、JaVT等病毒复合侵染,JaVH+JaVT复合侵染类型检出率最高,为26.32%。本研究首次在茉莉花上发现了CMV的感染,为了探明云南CMV茉莉花分离物与来自其他地区和寄主植物CMV分离物的分子变异和系统发育情况,将获得的CMV茉莉花分离物(CMV-YYJMLH)的CP基因序列(GenBank登录号:OQ870529)与GenBank中报道的其他35个CMV分离物CP基因序列进行比对分析,其核苷酸序列一致性为77.17% ~ 99.09%,其中CMV-YYJMLH与CMV云南辣椒分离物(GenBank登录号:MT786689)的一致性最高,为99.09%。将不同来源的CMV分离物CP基因序列构建系统发育树,结果显示,CMV-YYJMLH分离物属于亚组I中的IB亚组,该分离物与CMV云南辣椒分离物亲缘关系最近。这是国内外首次报道CMV侵染茉莉花,也是JaVT在云南首次被发现的正式报道。
其他语种文摘 From 2021 to 2022, jasmine plants with virus-like symptoms of mosaic, yellowing, chlorosis, ringspot and mosaic were observed in Yuanjiang county, Yuxi city and Chenggong district, Kunming city in Yunnan Province, and a total of 95 symptomatic jasmine samples were collected. RT-PCR detection was performed using the virus universal primers of genera Begomovirus, Luteovirus, Polerovirus, Potexvirus, Potyvirus, Tobamovirus and Umbravirus, and the specific primers of cucumber mosaic virus (CMV), jasmine mosaic-associated virus (JMaV), jasmine virus C (JaVC), jasmine virus H (JaVH), jasmine virus T (JaVT) and tobacco streak virus (TSV) for the diseased jasmine samples. The results showed that the diseased jasmine samples collected in Yuanjiang county of Yuxi city and Chenggong district of Kunming city were infected with JaVT, JaVH, JMaV, JaVC and CMV with the detection rates of 60.00%, 57.89%, 15.79%, 13.68% and 6.32%, respectively. JaVT and JaVH were the dominant viruses infecting jasmine in Yunnan. All the five viruses occurred in Yuanjiang and Chenggong, and the dominant viruses in these two regions were JaVH and JaVT. Meanwhile, the virus detection rate in Chenggong was lower than that in Yuanjiang. Viruses mostly cause damage to jasmine in the form of mixed infection, with 11 types of co-infection in the 5 jasmine viruses. JaVC and CMV are usually co-infected with viruses such as JaVH and JaVT. The co-infection of JaVH+JaVT had the highest detection rate at 26.31%. This study was the first to detect CMV infection in jasmine. To gain deeper insights into the molecular variation and phylogenetic relationship between the CMV jasmine isolate (CMV-YYJMLH) obtained from Yunnan Province and other CMV isolates found in different regions and host plants, the cp gene sequence of CMV-YYJMLH isolate (GenBank accession number: OQ870529) was compared and analyzed with those of 35 other CMV isolates documented in the GenBank. The results showed that isolate YYJMLH shared 77.17%-99.09% nucleotide (nt) sequence identity with other CMV isolates, among them, isolate YYJMLH had the highest nt sequence identity of 99.09% with the CMV pepper isolate (GenBank accession number: MT786689) from Yunnan province. A phylogenetic tree was constructed using the cp gene sequences of different CMV isolates, and results showed that the CMV-YYJMLH jasmine isolate belonged to CMV subgroup I, and the YYJMLH isolate had the closest genetic relationship with the CMV pepper isolate (GenBank accession number: MTT86689). To our knowledge, this is the first report of CMV naturally infecting jasmine worldwide and the first record of JaVT in Yunnan province.
来源 植物病理学报 ,2024,54(4):713-725 【核心库】
DOI 10.13926/j.cnki.apps.001343
关键词 茉莉花 ; 病毒鉴定 ; 黄瓜花叶病毒 ; 系统发育分析
地址

1. 云南农业大学植物保护学院, 昆明, 650201  

2. 元江县植保植检站, 元江, 653300  

3. 云南农业大学现代教育技术中心, 昆明, 650201

语种 中文
文献类型 研究性论文
ISSN 0412-0914
学科 植物保护
基金 国家重点研发计划 ;  云南省院士专家工作站
文献收藏号 CSCD:7863540

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1 曹玉莲 侵染蝴蝶兰的病毒检测及ToMMV蝴蝶兰分离物的cp基因序列分析 云南农业大学学报,2025,40(1):42-50
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