Response of linear and cyclic electron flux to moderate high temperature and high light stress in tomato
线性电子传递和环式电子传递对缓解番茄亚高温强光胁迫的响应
查看参考文献58篇
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文摘
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Objective: To evaluate the possible photoprotection mechanisms of cyclic and linear electron flux (CEF and LEF) under specific high temperature and high light (HH) stress. Methods: Six-leaf-stage tomato seedlings (“Liaoyuanduoli”, n=160) were divided into four parts: Part 1, served as control under 25 ℃, 500 μmol/(m~2·s); Part 2, spayed with distilled water (H_2O) under 35 ℃, 1000 μmol/(m~2·s) (HH); Part 3, spayed with 100 μmol/L diuron (DCMU, CEF inhibitor) under HH; Part 4, spayed with 60 μmol/L methyl viologen (MV, LEF inhibitor) under HH. Energy conversion, photosystem I (PSI), and PSII activity, and trans-thylakoid membrane proton motive force were monitored during the treatment of 5 d and of the recovering 10 d. Results: HH decreased photochemical reaction dissipation (P) and the maximal photochemical efficiency of PSII (F_v/F_m), and increased the excitation energy distribution coefficient of PSII (β); DCMU and MV aggravated the partition imbalance of the excitation energy (γ) and the photoinhibition degree. With prolonged DCMU treatment time, electron transport rate and quantum efficiency of PSI (ETR_I and Y_I) significantly decreased whereas acceptor and donor side limitation of PSI (Y_(NA) and Y_(ND)) increased. MV led to a significant decline and accession of yield of regulated and non-regulated energy YNPQ and YNO, respectively. Membrane integrity and ATPase activity were reduced by HH stress, and DCMU and MV enhanced inhibitory actions. Conclusions: The protective effects of CEF and LEF were mediated to a certain degree by meliorations in energy absorption and distribution as well as by maintenance of thylakoid membrane integrity and ATPase activity. |
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其他语种文摘
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目的:探讨特定高温和强光逆境下番茄叶片中的环式电子传递(CEF)和线性电子传递(LEF)的光保护机制。创新点:通过引入电子抑制剂的方法系统分析了CEF和LEF对亚高温强光胁迫的响应。方法:将品种为“辽园多丽”的番茄幼苗(n=160)平均分成四组(表1):组1,于常温常光照25 ℃, 500 μmol/(m~2·s)条件下培养并作为对照;组2,叶片喷施蒸馏水并在亚高温强光35 ℃, 1000 μmol/(m~2·s)(HH)条件下培养;组3,HH条件下叶片喷施100 μmol/L敌草隆(DCMU,CEF抑制剂);组4,HH条件下叶片喷施60 μmol/L甲基紫精(MV,LEF抑制剂)。在处理5 d及恢复10 d期间,分别测定番茄幼苗叶片的光能吸收、激发能分配、光系统活性、类囊体膜完整性和ATP酶活性等指标。结论:CEF和LEF通过一定程度上改善叶片光能吸收及激发能分配,并且维持类囊体膜较高完整性和ATP酶活性,从而维持光系统活性并减少光抑制和光破坏程度。 |
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来源
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Journal of Zhejiang University. Science B
, Biomedicine & Biotechnology,2017,18(7):635-648 【核心库】
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DOI
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10.1631/jzus.b1600286
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关键词
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Photoinhibition
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Photoprotection
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Thylakoid membrane
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Tomato
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地址
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College of Horticulture, Shenyang Agricultural University, Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province;;Collaborative Innovation Center of Protected Vegetable Surrounds Bohai Gulf Region, Shenyang, 110866
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语种
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英文 |
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文献类型
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研究性论文 |
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ISSN
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1673-1581 |
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学科
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园艺 |
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基金
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Project supported by the China Agriculture Research System
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国家自然科学基金
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国家科技攻关计划项目
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文献收藏号
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CSCD:6034514
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