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Evaluation of three tracers for labeling distal cerebrospinal fluidcontacting neurons

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文摘 It has been reported that distal cerebrospinal fluid-contacting neurons (dCSF-CNs) can be detected by immunohistochemical assay using cholera toxin subunit B-conjugated horseradish peroxidase (CBHRP). In the present study, another two methods with CB alone or CB-conjugated FITC (CB-FITC) were used, and the results from the three methods were compared. Adult Sprague-Dawley rats were randomly divided into three groups with CB-HRP, CB or CB-FITC. Tracers were diluted to 30% in artificial cerebrospinal fluid and injected separately (in a volume of 3 μL) into the lateral ventricle. Animals from the CB-HRP and CB groups were perfused 48 h after surgery while animals from the CB-FITC group were perfused 1, 3, 6, 12, 24 or 48 h after surgery. The brain was sectioned (40 μm) for immunofluorescence and five sections with positive neurons were selected from each rat for neuron counting. Three clusters of positive neurons in a 'Y-like' distribution were detected ventral to the cerebral aqueduct of rats from the three groups. No significant difference was observed among the quantitative data. In the CB-FITC group, stable staining was detected even at 6 h after injection. Taken together, lateral ventricle injection of CB/CB-FITC is a useful method for labeling dCSFCNs in rats. The CB-FITC method makes dCSF-CNs labeling much simpler and more convenient.
来源 Neuroscience Bulletin ,2013,29(5):576-580 【核心库】
DOI 10.1007/s12264-013-1332-0
关键词 distal cerebrospinal fluid-contacting neurons ; cholera toxin subunit B-conjugated horseradish peroxidase ; cholera toxin subunit B ; cholera toxin subunit B-conjugated FITC ; label
地址

1. Department of Anesthesiology, The First Clinical College, China Medical University, Jiangsu Province Key Laboratory of Anesthesiology;;Jiangsu Key Laboratory of Brain Disease Bioinformation, Shenyang, 110001  

2. Xuzhou Medical College, Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou, 221002  

3. Department of Anesthesiology, The First Clinical College, China Medical University, Jiangsu Province Key Laboratory of Anesthesiology, Shenyang, 110001

语种 英文
文献类型 研究性论文
ISSN 1673-7067
学科 外科学
基金 the Natural Science Foundation of the Jiangsu Higher Education Institutions of China ;  Open Project Funds of the Jiangsu Key Laboratory of Brain Disease Bioinformation ;  the Fund of Science and Technology Innovation Team in Jiangsu Province, China (2009) ;  国家自然科学基金
文献收藏号 CSCD:4975289

参考文献 共 14 共1页

1.  Vigh B. Structure of the medullo-spinal liquor-contacting neuronal system. Acta Biol Acad Sci Hung,1971,22:227-243 CSCD被引 1 次    
2.  Zhang L C. The distributions and signaling directions of the cerebrospinal fluid contacting neurons in the parenchyma of a rat brain. Brain Res,2003,989:1-8 CSCD被引 33 次    
3.  Lu X. The methodology for labeling the distal cerebrospinal fluid-contacting neurons in rats. J Neurosci Methods,2008,168:98-103 CSCD被引 9 次    
4.  Wu T T. Distribution of TRPC6 in the cerebrospinal fluid-contacting nucleus of rat brain parenchyma and its expression in morphine dependence and withdrawal. Neurochem Res,2011,36:2316-2321 CSCD被引 4 次    
5.  Vigh B. The system of cerebrospinal fluid-contacting neurons. Its supposed role in the nonsynaptic signal transmission of the brain. Histol Histopathol,2004,19:607-628 CSCD被引 19 次    
6.  Du J. Expression of TRPM8 in the distal cerebrospinal fluid-contacting neurons in the brain mesencephalon of rats. Cerebrospinal Fluid Res,2009,6:3 CSCD被引 3 次    
7.  Zhang L. Effects of morphine-dependent and withdrawal on activation of the distal cerebrospinal fluid contacting neurons' phosphorylation CREB in rat brain. Neurol Res,2009,31:738-742 CSCD被引 5 次    
8.  Lu X F. Substance P in the cerebrospinal fluid-contacting nucleus contributes to morphine physical dependence in rats. Neurosci Lett,2011,488:188-192 CSCD被引 6 次    
9.  Ishihara A. Retrograde neuronal labeling of motoneurons in the rat by fluorescent tracers, and quantitative analysis of oxidative enzyme activity in labeled neurons. Neurosci Lett,1991,124:141-143 CSCD被引 1 次    
10.  Alibardi L. H3-thymidine labeled cerebrospinal fluid contacting cells in the regenerating caudal spinal cord of the lizard Lampropholis. Ann Anat,1994,176:347-356 CSCD被引 1 次    
11.  Ekstrom P. Photoreceptors and CSF-contacting neurons in the pineal organ of a teleost fish have direct axonal connections with the brain: an HRP-electron-microscopic study. J Neurosci,1987,7:987-995 CSCD被引 1 次    
12.  Kobbert C. Current concepts in neuroanatomical tracing. Prog Neurobiol,2000,62:327-351 CSCD被引 8 次    
13.  Tang F R. A light-microscopic study of the intermediolateral nucleus following injection of CB-HRP and fluorogold into the superior cervical ganglion of the rat. J Auton Nerv Syst,1995,50:333-338 CSCD被引 1 次    
14.  Kopitz J. Ganglioside GM1/galectin-dependent growth regulation in human neuroblastoma cells: special properties of bivalent galectin-4 and significance of linker length for ligand selection. Neurochem Res,2012,37:1267-1276 CSCD被引 1 次    
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