海南红麻曲叶病的病原检测与鉴定
Detection and identification of the pathogen causing kenaf (Hibiscus cannabinus) leaf curl disease in Hainan Province of China
查看参考文献25篇
|
文摘
|
红麻曲叶病是2012年在海南省海口市发现的一种新病害,病株表现为叶片向上卷曲、叶脉肿大、叶脉变深绿色等症状。PCR检测结果显示,该病样中均存在菜豆金色花叶病毒属病毒。基因克隆及序列分析结果表明,该病毒分离物(HN08)基因组仅含A组分(DNA-A),其全长为2 738 nt,与木尔坦棉花曲叶病毒(CLCuMuV)各分离物的相似性均大于89.0 %,其中与中国各分离物的相似性均大于99.0 %。该病毒分离物也伴随有β卫星分子,其全长为1 346 nt,与CLCu-MuV各分离物伴随的β卫星分子(CLCuMuB)序列相似性大于83.0 %,其中与分离物Fzl的序列相似性最高,为99.7 %。构建了HN08 DNA-A及其β卫星分子侵染性克隆,通过农杆菌注射接种红麻,接种后30 d,HN08 DNA-A及其β卫星分子混合接种的红麻植株新出叶片开始产生曲叶症状;接种后60 d,二者混合接种的植株大部分叶片表现为严重的曲叶症状,且与田间自然病株症状相同,而二者各自单独接种的红麻植株没有产生明显的症状。PCR及Southern blot检测进一步证实这些症状是由HN08DNA-A及其β卫星分子的共同侵染引起的。因此,海南红麻曲叶病是由CLCuMuV及其伴随的β卫星分子(CLCuMuB)共同侵染引起的。本文首次报道了红麻是CLCuMuV自然新寄主。 |
|
其他语种文摘
|
Kenaf leaf curl disease is a new disease occurred in Haikou, Hainan Province of China. The symptoms of the infected plants exhibits leaf curling upwards, vein swelling and vein dark green. PCR analysis indicates that the symptomatic plants were infected by begomoviruses and sequence analysis indicates that the isolate HN08 only contains the DNA-A component.The full length of DNA-A was 2 738 nucleotides. Multiple sequence alignment showed that the DNA-A of HN08 had more than 89.0% sequence identity with that of all other isolates of Cotton leaf curl Multan virus (CLCuMuV) deposited in GenBank,and more than 99.0% sequence identity with that of all isolates from China. The virus was also accompanied by betasatellite molecular, which was determined to be 1 346 nucleotides. Pairwise comparison indicated that the betasatellite molecular of HN08 had more than 83.0% sequence identity with that of Cotton leaf curl Multan betasatellite (CLCuMuB), and shared the highest sequence identity (99.7%) with that of isolate Fzl from Fujian. The infectious clones of HN08 DNA-A and its betasatellite molecular, pGreenII049-1.6A and pGreenII049-2.0β were constructed, and were agro-inoculated into Hibiscus cannabinus plants. At 30 dpi, the new leaves of the H. cannabinus plants inoculated with the recombination of pGreenII049-1.6A and pGreenII049-2.0β displayed leaf curl symptoms. At 60 dpi,most of the leaves of the inoculated H. cannabinus plants showed severe leaf curl symptoms, similar to that observed in the fields. However, neither the plants inoculated with pGreenII049-1.6A or pGreenII049-2.0β showed visible symptoms. The results of PCR and Southern blot detection confirmed these symptoms caused by co-infection of HN08 DNA-A and its betasatellite molecular. Taken together, Kenaf leaf curl disease in Hainan province was caused by co-infection of CLCuMuV and its betasatellite (CLCuMuB). This is the first report of CLCuMuV on H. cannabinus. |
|
来源
|
植物病理学报
,2015,45(6):561-568 【核心库】
|
|
DOI
|
10.13926/j.cnki.apps.2015.06.001
|
|
关键词
|
红麻曲叶病
;
木尔坦棉花曲叶病毒
;
卫星分子
;
病原鉴定
|
|
地址
|
广东省农业科学院植物保护研究所, 广东省植物保护新技术重点实验室, 广州, 510640
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
0412-0914 |
|
学科
|
植物保护 |
|
基金
|
广东省科技计划项目
;
广东省广州市科技计划项目
;
广东省农业科学院院长基金
|
|
文献收藏号
|
CSCD:5594419
|
参考文献 共
25
共2页
|
|
1.
Briddon R W. Cotton leaf curl virus disease.
Virus Research,2000,71(1/2):151-159
|
CSCD被引
28
次
|
|
|
|
|
2.
Zhou X P. Four DNA-A variants among Pakistani isolates of cotton leaf curl virus and their affinities to DNA-A of geminivirus isolates from okra.
Journal of General Virology,1998,79(4):915-923
|
CSCD被引
30
次
|
|
|
|
|
3.
Mansoor S. Cotton leaf curl disease is associated with multiple monopartite be-gomoviruses supported by single DNA β.
Archives of Virology,2003,148(10):1969-1986
|
CSCD被引
18
次
|
|
|
|
|
4.
Fauquet C M. Geminivirus strain demarcation and nomenclature.
Archives of Virology,2008,153(4):783-821
|
CSCD被引
47
次
|
|
|
|
|
5.
Rajeshwari R. Host range,vector relationships and sequence comparison of a begomovirus infecting hibiscus in India.
Annals of Applied Biology,2005,147(1):15-25
|
CSCD被引
3
次
|
|
|
|
|
6.
Cai J H. Cotton leaf curl Multan virus newly reported to be associated with cotton leaf curl disease in China.
Plant Pathology,2010,59(4):794-795
|
CSCD被引
23
次
|
|
|
|
|
7.
Mao M J. Molecular characterization of Cotton leaf curl Multan virus and its satellite DNA that infects Hibiscus rosa-sinensis (in Chinese).
病毒学报,2008,24(1):64-68
|
CSCD被引
4
次
|
|
|
|
|
8.
Tang Y F. Molecular characterization of the Cotton leaf curl Multan virus infecting Malvaiscus arboreus(in Chinese).
植物病理学报,2013,43(2):113-119
|
CSCD被引
1
次
|
|
|
|
|
9.
Dong D. Molecular characterization of Cotton leaf curl Multan virus and the associated satellite DNA infecting Okra in Guangdong (in Chinese).
华南农业大学学报,2012,33(1):33-39
|
CSCD被引
4
次
|
|
|
|
|
10.
Sinha V. Association of Cotton leaf curl Multan virus and its satellite molecules with leaf curl disease of papaya in India.
New Disease Reports,2013,27:9
|
CSCD被引
1
次
|
|
|
|
|
11.
Briddon R W. Cotton leaf curl disease, a multicompo-nent begomovirus complex.
Molecular Plant Pathology,2003,4(6):427-434
|
CSCD被引
15
次
|
|
|
|
|
12.
Briddon R W. Identification of DNA components required for induction of cotton leaf curl disease.
Virology,2001,285(2):234-243
|
CSCD被引
35
次
|
|
|
|
|
13.
Chatterjee A. A new monopartite begomovirus isolated from Hibiscus cannabinus L. in India.
Archives of Virology,2007,152(11):2113-2118
|
CSCD被引
1
次
|
|
|
|
|
14.
Roy A. Distribution, epidemiology and molecular variability of the begomovirus complexes associated with yellow vein mosaic disease of mesta in India.
Virus Research,2009,141(2):237-246
|
CSCD被引
2
次
|
|
|
|
|
15.
Raj S K. Yellow vein netting of Bimili jute (Hibiscus cannabinus L.) in India caused by a strain of Tomato leaf curl New Delhi virus containing DNAβ.
Australasian Plant Disease Notes,2007,2(1):45-47
|
CSCD被引
1
次
|
|
|
|
|
16.
Paul S. First report of Tomato leaf curl Joydebpur virus and associated betasatellite in kenaf (Hibiscus cannabinus) plants showing leaf curl symptoms from southern India.
Plant Pathology,2009,58(2):403
|
CSCD被引
1
次
|
|
|
|
|
17.
Lin P Q. Some thoughts on strengthening system construction of kenaf seed industry system and products to comprehensive development (in Chinese).
福建农业科技,2000,6:26-27
|
CSCD被引
1
次
|
|
|
|
|
18.
Wyatt S D. Detection of subgroup III geminivirus isolates in leaf extracts by degenerate primers and polymerase chain reaction.
Phytopathology,1996,86(12):1288-1293
|
CSCD被引
23
次
|
|
|
|
|
19.
He Z F. Detection of whitefly-trans-mitted geminiviruses from tomato by PCR(in Chinese).
中国病毒学,2004,19(1):67-69
|
CSCD被引
3
次
|
|
|
|
|
20.
Doyle J J. Isolation of plant DNA from fresh tissue.
Focus,1990,12:13-15
|
CSCD被引
401
次
|
|
|
|
|
了解气象卫星更多信息，请访问