黄芪对代谢综合征大鼠心脏血管紧张素1-7受体Mas表达的影响
The effect of Radix Astragali on angiotensin 1-7 receptor expression in myocardium of metabolic syndrome rats
查看参考文献23篇
|
文摘
|
目的探讨黄芪影响代谢综合征(MS)大鼠心脏血管紧张素1-7(Ang 1-7)受体Mas蛋白的表达及其抗氧化应激心肌保护的作用。方法雄性SD大鼠120只分为正常对照组、MS组、MS+黄芪组,采用两肾一夹法及配合高脂饲料和饮用果糖水的方法构建MS大鼠模型。术后MS+黄芪组予以黄芪6.0 g/(kg·d)灌胃治疗。4周后,行超声心动图及有创血流动力学检测左心室功能;放免法检测血清及心肌血管紧张素Ⅱ(Ang Ⅱ)、丙二醛、超氧化物歧化酶(SOD)水平,蛋白免疫印迹法检测各组大鼠心肌Mas受体、血管紧张素转换酶(ACE)和ACE2的表达。结果与正常对照组比较,MS组与MS+黄芪组大鼠收缩压、舒张压、体质量、空腹血糖、空腹胰岛素水平、三酰甘油、血清游离脂肪酸以及血浆Ang Ⅱ水平明显升高(均P<0.05);心肌SOD活性下降[(106.34±16.07),(141.06±23.20)比(174.02±20.52 )U/mg,均P<0.05];心肌丙二醛水平升高[(30.37±7.43),(22.43±5.25)比(17.56±2.49)mmol/g,均P<0.05]。与MS组比较,MS+黄芪组血浆Ang Ⅱ水平和心肌丙二醛水平降低(分别P=0.001和0.017);心肌SOD活性升高(P=0.006)。血流动力学参数结果显示:MS+黄芪组大鼠左心室内压最大收缩和舒张变化速率较MS组升高(均P<0.05)。与对照组比较,MS组心肌组织中ACE表达升高,而ACE2和Mas受体的表达降低(均P<0.05);与MS组比较,MS+黄芪组心肌组织中ACE表达降低,而ACE2和Mas受体的表达升高(均P<0.05)。结论黄芪可以提高心肌组织ACE2、Mas的表达, 降低ACE表达,改善心脏局部ACE2、Mas的水平,从而对损伤的心肌细胞起保护作用。 |
|
其他语种文摘
|
Objective To clarify the anti-oxidative role of Radix Astragali and its effect on angiotensin (Ang) 1-7 specific receptor, Mas, in metabolic syndrome (MS) rats. Methods A total of 120 male SD rats were divided into three groups: the normal control (NC) group, the MS group and the MS+Radix Astragali group [MS+RA, 6.0 mg/(kg·d) in gavage]. The two-kidney, one-clip method with high-fat diets and fructose water was constructed to mimic the MS model. After four-week treatment, hemodynamic and echocardiographic parameters were used to assess left ventricular functions. Plasma and myocardial Ang Ⅱ, malondialdehyde and superoxide demutase (SOD) levels were measured with radioimmunoassay. The protein levels of Mas, angiotensin converting enzyme (ACE) and ACE2 were detected by Western blots. Results Compared with the NC group, systolic and diastolic pressure, body weight, fasting glucose, fasting insulin, triglycerides, free fatty acid, Ang Ⅱ and myocardial malondialdehyde levels [(30.37±7.43) and (22.43±5.25) vs (17.56±2.49) mmol/g) were significantly increased in both MS and MS+RA groups (all P<0.05), while myocardial SOD activity was decreased [(106.34±16.07) and (141.06±23.20) vs (174.02±20.52) U/mg, P<0.05]. Plasma Ang Ⅱ and myocardial malondialdehyde levels were lower in the MS+RA group than in the MS group (P=0.001 and 0.017, respectively), while myocardial SOD activity was higher (P=0.006). Left ventricular internal pressure ±dp/dt_(max) was higher in the MS+RA group than in the MS group (both P<0.05). In myocardial tissue, ACE protein expression was increased in the MS group compared with the NC group, while ACE2 and Mas receptor expressions were decreased (all P<0.05). Compared with the MS group, ACE protein expression in myocardial tissue was decreased, while ACE2 and Mas receptor expressions were increased in the MS+RA group (all P<0.05). Conclusion Radix Astragali can increase the ACE2 and Mas receptor expressions, which has implications for new therapeutic in metabolic syndrome. |
|
来源
|
中华高血压杂志
,2015,23(2):168-173 【核心库】
|
|
关键词
|
黄芪
;
代谢综合征
;
氧化应激
;
Mas受体
|
|
地址
|
1.
兰州大学第二医院心内科, 甘肃, 兰州, 730030
2.
兰州大学第二医院危重病科, 甘肃, 兰州, 730030
|
|
语种
|
中文 |
|
ISSN
|
1673-7245 |
|
学科
|
生物化学;医药、卫生;内科学 |
|
基金
|
甘肃省中医药管理局重点课题
;
兰州大学附属第二医院基金
|
|
文献收藏号
|
CSCD:5390330
|
参考文献 共
23
共2页
|
|
1.
Grundy S M. Diagnosis and management of the metabolic syndrome:an American Heart Association/National Heart, Lung, and Blood Institute Scientific Statement.
Circulation,2005,112(17):2735-2752
|
被引
150
次
|
|
|
|
|
2.
Coelho M S. High sucrose intake in rats is associated with increased ACE2 and angiotensin-(1-7) levels in the adipose tissue.
Regul Pept,2010,162(1/3):61-67
|
被引
3
次
|
|
|
|
|
3.
Marcus Y. Angiotensin 1-7 as means to prevent the metabolic syndrome:lessons from the fructose-fed rat model.
Diabetes,2013,4(62):1112-1130
|
被引
2
次
|
|
|
|
|
4.
Passos-Silva D G. Angiotensin-(1-7):beyond the cardio-renal actions.
Clin Sci(Lond),2013,124(7):443-456
|
被引
11
次
|
|
|
|
|
5.
宗文纳. 血管紧张素(1-7)/Mas的研究进展.
国际心血管病杂志,2010,37(3):160-168
|
被引
1
次
|
|
|
|
|
6.
Qiu L H. Astragaloside Ⅳ improves homocysteine-induced acute phase endothelial dysfunction via antioxidation.
Biol Pharm Bull,2010,33(4):641-646
|
被引
13
次
|
|
|
|
|
7.
甄玲玲. 甘肃黄芪浸膏粉对代谢综合征大鼠心脏血管紧张素转换酶2表达的影响.
中华高血压杂志,2010,18(8):727-732
|
被引
1
次
|
|
|
|
|
8.
王立文. 左侧直入路二肾一夹法复制肾血管性高血压大鼠动物模型.
第三军医大学学报,1998,20(1):39-41
|
被引
1
次
|
|
|
|
|
9.
霍勇.
心血管疾病实验动物学,2011:222-225
|
被引
1
次
|
|
|
|
|
10.
Donoghue M. A novel angiotensin-converting enzyme related carboxypeptidase (ACE2) converts angiotensin Ⅰ to angiotensin(1-9).
Circ Res,2000,87(5):E1-E9
|
被引
160
次
|
|
|
|
|
11.
Whaley-Connell A. Oxidative stress in the cardiorenal metabolic syndrome.
Curr Hypertens Rep,2012,14(4):360-365
|
被引
4
次
|
|
|
|
|
12.
蒋毅弘. 血管紧张素转换酶2、氧化应激与高血压.
中华高血压杂志,2011,19(6):518-520
|
被引
11
次
|
|
|
|
|
13.
Zhong J. Angiotensin-converting enzyme 2 suppresses pathological hypertrophy, myocardial fibrosis, and cardiac dysfunction.
Circulation,2010,122(7):717-728
|
被引
24
次
|
|
|
|
|
14.
Zong W N. Regulation of angiotensin-(1-7) and angiotensin II type 1 receptor by telmisartan and losartan in adriamycin-induced rat heart failure.
Acta Pharmacol Sin,2011,32(11):1-6
|
被引
1
次
|
|
|
|
|
15.
卞伟华. Mas功能及其信号转导通路的研究进展.
生命科学,2013,25(5):497-500
|
被引
2
次
|
|
|
|
|
16.
Esterbauer H. The role of lipid peroxidation and antioxidants in oxidative modification of LDL.
Free Radic Biol Med,1992,13(4):341-390
|
被引
12
次
|
|
|
|
|
17.
Mottillo S. The metabolic syndrome and cardiovascular risk:a systematic review and meta-analysis.
J Am Coll Cardiol,2010,56(14):1113-1132
|
被引
93
次
|
|
|
|
|
18.
Inaba S. Role of angiotensin-converting enzyme 2 in cardiac hypertrophy induced by nitric oxide synthase inhibition.
J Hypertens,2011,29(11):2236-2245
|
被引
4
次
|
|
|
|
|
19.
Xu M E. Effects of astragaloside Ⅳ on pathogenesis of metabolic syndrome in vitro.
Acta Pharmacol Sin,2006,27(2):229-236
|
被引
6
次
|
|
|
|
|
20.
Zhang W D. Astragaloside Ⅳ from astragalus membranaceus shows cardioprotection during myocardial ischemia in vivo and in vitro.
Planta Medica,2006,72(1):4-8
|
被引
27
次
|
|
|
|
|
了解气象卫星更多信息，请访问