花椒根腐病的病原鉴定与防治药剂筛选
Pathogen identification of root rot of Zanthoxylum bungeanum and screening of fungicides
查看参考文献28篇
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文摘
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近几年,根腐病在我国花椒产区大面积发生,严重影响了花椒的产量和质量。为明确引起花椒根腐病的病原,本研究从陕西省凤县花椒示范基地采集花椒根腐病标本,采用组织分离法和致病性检验获得花椒根腐病菌。通过对花椒根腐病菌的形态学观察及ITS、β-tubulin和TEF1-α序列分析,将花椒根腐病菌鉴定为腐皮镰刀菌(Fusarium solani) 。杀菌剂室内抑菌活性筛选结果表明,选用的十种杀菌剂对F. solani的菌落生长均表现出一定的抑制作用,其中以6%丙唑·多菌灵悬乳剂、30%苯甲·丙环唑悬乳剂、50%多菌灵可湿性粉剂和80%代森锰锌可湿性粉剂对花椒根腐病菌的抑制作用较强,EC_(50)值分别为0.48、0.66、1.40和1.61 mg·L~(-1)。本研究结果为花椒根腐病的有效防控提供了科学依据。 |
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其他语种文摘
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In recent years,root rot has occurred in the main Zanthoxylum bungeanum cultivation regions,which seriously affects the yield and quality of Z. bungeanum. To identify the pathogen of this disease,the diseased roots were collected from a demonstration base in Fengxian County,Shaanxi Province. The pathogen was obtained by tissue separation method and pathogenicity test. Based on the morphological observation and sequence analyses of internal transcribed spacer (ITS),β-tubulin and translation elongation factor 1-α (TEF1-α), Fusarium solani was identified as the pathogen causing Z. bungeanum root rot. The experiments for the antifungal activity screening in vitro showed that all the ten fungicides selected in this study showed certain inhibitory effects on the colony growth of F. solani,where 6% Prothioconazole and carbendazol SC,30% Pyraclostrobin and tebuconazole SC,50% Carbendazim WP and 80% Mancozeb WP exhibited relative higher inhibitory effects with EC_(50) as 0.48,0.66,1.40 and 1.61 mg·L~(-1),respectively. The results presented herein provide useful guidance for the effective control of Z. bungeanum root rot. |
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来源
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植物病理学报
,2022,52(4):630-637 【核心库】
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DOI
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10.13926/j.cnki.apps.000770
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关键词
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花椒
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根腐病
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病原鉴定
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腐皮镰刀菌
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杀菌剂
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地址
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西北农林科技大学林学院, 西部森林生物灾害治理国家林业和草原局重点试验室, 杨凌, 712100
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0412-0914 |
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学科
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林业 |
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基金
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陕西省重点研发计划
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西北农林科技大学科技创新与成果转化项目
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文献收藏号
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CSCD:7336451
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