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醚菌酯对假禾谷镰孢的抑制作用及对小麦茎基腐病的防效研究
Inhibitory effect of kresoxim-methyl on Fusarium pseudograminearum and control effect on crown rot of wheat

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王莉莉 1,2   徐建强 1 *   朱凯 1   孙莹 1   郑伟 1   侯颖 3 *  
文摘 为了明确醚菌酯对假禾谷镰孢的抑制作用,本研究采用菌丝生长速率法和孢子萌发抑制法分别测定了醚菌酯对2019年从河南省13个地市分离的50株假禾谷镰孢的毒力。两种方法结果表明,醚菌酯对供试50株菌株的EC_(50)值范围分别在0.127 3~ 3.070 9 μg·mL~(-1)和0.001 3 ~ 0.070 9 μg·mL~(-1),平均EC_(50)值为(1.304 1±0.804 2)μg·mL~(-1)和(0.017 4±0.017 0) μg·mL~(-1) 。敏感性频率分布图显示,两种方法分别有80%(40株)和70%(35株)的菌株敏感性频率呈正态分布,其平均EC_(50)值分别为(1.135 2±0.531 2)μg·mL~(-1)和(0.011 4±0.006 3)μg·mL~(-1),可作为假禾谷镰孢对醚菌酯的敏感性基线。方差分析结果表明,同一县市的假禾谷镰孢对醚菌酯的敏感性差异较大,EC_(50)值变化范围为0.545 2~ 2.156 4 μg·mL~(-1)和0.001 9~ 0.036 7 μg·mL~(-1) 。聚类分析结果显示,河南省假禾谷镰孢对醚菌酯敏感性差异与菌株的地理来源无明显关联性。温室防效结果显示,小麦种子与醚菌酯悬浮种衣剂进行拌种处理可以起到一定防治效果,0.42 μL AI / 2g处理防效最高,防治效果可达50.33%。本研究结果可为河南省假禾谷镰孢对醚菌酯的敏感性监测提供信息,同时为醚菌酯对小麦茎基腐病的防治提供理论依据。
其他语种文摘 In this study, the inhibitory effect of kresoxim-methyl to 50 Fusarium pseudograminearum strains isolated from 13 cities in Henan Province in 2019 based on the mycelial growth and spore germination methods, respectively. The results showed that the EC_(50) values of kresoxim-methyl to the 50 tested strains were in the range of 0.127 3-3.070 9 μg·mL~(-1) and 0.001 3-0.070 9 μg·mL~(-1) for the two methods, respectively. And the average EC_(50) values were(1.304 1±0.804 2) μg·mL~(-1) and(0.017 4±0.017 0) μg·mL~(-1), respectively. 80% and 70% of the tested strains were with continuous single peak frequency distribution and their average EC_(50) values at(1.135 2 ±0.531 2) μg·mL~(-1) and(0.011 4±0.006 3) μg·mL~(-1), respectively, which could be used as the baseline sensitivity of F. pseudograminearum to kresoxim-methyl for the two methods. The sensitivity to kresoxim-methyl was varied for the strains from the same city with the EC_(50) values in the range of 0.545 2-2.156 4 μg·mL~(-1) and 0.001 9-0.036 7 μg·mL~(-1) for the two methods, respectively. The results of cluster analysis showed that there was no significant correlation between the sensitivity of F. pseudograminearum strains to kresoxim-methyl and their geographical origin. The wheat seeds mixed with kresoxim-methyl suspension had a certain control effect in a greenhouse test where 0.42 μL AI / 2g treatment had the highest control effect at 50.33%. The results of this study could provide a theoretical basis for the using of kresoxim-methyl in the control of wheat crown rot caused by F. pseudograminearum.
来源 植物病理学报 ,2022,52(3):434-442 【核心库】
DOI 10.13926/j.cnki.apps.000758
关键词 假禾谷镰孢 ; 醚菌酯 ; 小麦茎基腐病 ; 毒力测定 ; 温室防效
地址

1. 河南科技大学园艺与植物保护学院, 洛阳, 471003  

2. 华中农业大学植物科学技术学院, 武汉, 430070  

3. 河南科技大学食品与生物工程学院, 洛阳, 471003

语种 中文
文献类型 研究性论文
ISSN 0412-0914
学科 植物保护
基金 河南省科技攻关项目 ;  旱区作物逆境生物学国家重点实验室开放课题研究计划 ;  国家级大学生创新创业训练计划
文献收藏号 CSCD:7233911

参考文献 共 19 共1页

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引证文献 6

1 李怡文 假禾谷镰孢引起的小麦茎基腐病发生危害与防控研究进展 农药学学报,2022,24(5):949-961
CSCD被引 4

2 郭雨薇 嘧菌酯对河南省小麦茎基腐病菌的抑制活性及对病害的防治效果 农药学学报,2022,24(6):1409-1416
CSCD被引 3

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