铊在真菌亚细胞中的分布特征
Sub-cellular Distribution of Thallium in Fungus
查看参考文献25篇
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文摘
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将前期从铊污染区筛选得到的9株高耐受性真菌菌株用于微生物对铊的富集和亚细胞分布实验,采用酶解和差速离心法分离各亚细胞组分,并检测其中铊的质量分数.结果表明,生物富集量随着铊处理浓度上升而降低,其影响趋势与对生物量的影响趋势基本一致,最高可达7 189 mg/kg,最大富集系数为7.19.在亚细胞水平上,铊的富集优先顺序为:细胞质-细胞壁-细胞器.亚细胞水平的区隔化作用是真菌对铊的主要耐受机制,细胞质是赋存铊的主要场所(53.83%~79.45%). |
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其他语种文摘
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This paper focused on the sub-cellular distribution of thallium in several different fungus strains. The fungus strains were isolated from the Lanmuchang thallium-mineralised area in southwest Guizhou Province,and were used in the bio-concentration experiments. The sub-cellular supernatants including cytoplasmic,cellwall and cell organelles were seperated by the differential centrifuge after treated with snailase. Results show that the bio-concentration rate of thallium in the nine strains decreases with increasing thallium contents in the culture,the maximum at 7 189 mg /kg and the maximum bioconcentration factor (BCF) at 7. 19. In the sub-cellular supernatants detected by ICP-OES,the majority of thallium (up to 79. 45%) was found in cytoplasmic organelles,and the concentrations of thallium in the order of priority are as follows: cytoplasmic-cellwall-cell organelles. Cytoplasmic supernatant is apparently a major storage site for thallium,which is a possible detoxification of thallium by the fungus. |
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来源
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北京工业大学学报
,2013,39(6):941-947 【扩展库】
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关键词
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铊
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微生物
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真菌
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亚细胞
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分布
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富集
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地址
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1.
中国科学院地球化学研究所, 环境地球化学国家重点实验室, 贵阳, 550002
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贵州省环境科学研究设计院, 贵阳, 550002
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0254-0037 |
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学科
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地质学 |
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基金
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国家自然科学基金资助项目
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中国科学院知识创新工程重要方向项目
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文献收藏号
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CSCD:4854237
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