焦虑症肝郁化火的动物实验炎症机制研究
Inflammatory mechanism on pathogenic fire derived from stagnation of liver-qi in anxiety rats
查看参考文献13篇
|
文摘
|
目的:从炎症反应相关信号探讨焦虑症肝郁化火的形成机制。方法:采用单笼饲养和不确定性空瓶饮水应激建立肝郁化火焦虑模型。Wistar大鼠随机分成空白对照组、模型组,在应激第7天和14天用高架十字迷宫试验、旷场实验及明暗箱实验行为学评价,ELISA法测定外周血清和杏仁核中炎症因子IL-1β、IL-6、TNF-α、IL-10含量及mRNA表达水平。结果:模型组肝郁化火时(应激第14天)血清和杏仁核IL-1β、IL-6、TNF-α含量均显著增加(P<0.01),IL-10含量显著减少(P<0.01),杏仁核上述炎症因子mRNA与之类似,而肝气郁结时(应激第7天)仅有血清IL-1β、TNF-α含量及杏仁核IL-1β、IL-6 mRNA增加(P<0.05)。结论:在肝郁化火时,杏仁核及外周血炎症反应相关信号呈活化状态,促炎反应增加和抗炎反应减弱,免疫失衡,焦虑症肝郁化火实质是炎症。 |
|
其他语种文摘
|
Objective: To explore the formation mechanism of pathogenic fire derived from stagnation of liverqi (PFSL) in anxiety disorder through the inflammation related signal. Methods: Single cage rearing and stress of uncertain bottle of drinking water was used to prepare anxiety disorder rat model due to PFSL. Wistar rats were randomly divided into control group and model group. Elevated plus maze test, open field test and light/dark box test was used to assess anxiety behavior at seventh day and fourteenth day. Levels of inflammatory factors (IL-1β, IL-6, TNF-α, IL-10) and mRNA expression in peripheral blood and amygdala were detected by the ELISA method. Results: The levels of IL-1β, IL-6 and TNF-α in the serum and amygdala of model rats at the PFSL on the 14th day increased significantly (P<0.01) and the level of IL-10 decreased significantly observably (P<0.01). The mRNA expression level of these inflammatory factors in the amygdala was similar to the former. However, on the seventh day the levels of IL-1β and TNF-α in the serum and IL-1β and IL-6 in the amygdala at stagnation of liver qi increased merely (P<0.05). Conclusion: During anxiety disorder due to PFSL, the inflammatory response signals both in amygdala and serum of model rats deviated from normal range, which indicates that the inflammatory response at these sites is activated and inflammatory factors are involved in the process of injury and the neuroendocrine immunity network regulation. Proinflammatory response increases and anti-inflammatory response decreases. The essence of anxiety disorder with PFSL is inflammation. |
|
来源
|
中华中医药杂志
,2020,35(4):2010-2013 【扩展库】
|
|
关键词
|
肝郁化火
;
焦虑症
;
血清
;
杏仁核
;
炎症因子
|
|
地址
|
1.
北京中医药大学第三附属医院, 北京, 100029
2.
北京中医药大学东方医院, 北京, 100078
3.
中国科学院心理研究所, 北京, 100101
4.
北京中医药大学东直门医院, 北京, 101121
5.
北京市中西医结合医院, 北京, 100096
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1673-1727 |
|
学科
|
中国医学 |
|
基金
|
国家自然科学基金项目
|
|
文献收藏号
|
CSCD:6720677
|
参考文献 共
13
共1页
|
|
1.
唐启盛. 运用数据挖掘技术分析广泛性焦虑症的中医证候学规律.
中西医结合学报,2012,10(9):975-982
|
CSCD被引
10
次
|
|
|
|
|
2.
王椿野. 肝郁化火证的研究进展.
环球中医药,2012,5(2):138-142
|
CSCD被引
5
次
|
|
|
|
|
3.
赵振武. 肝郁化火证大鼠模型的建立与评价.
中华中医药杂志,2015,30(6):2050-2053
|
CSCD被引
7
次
|
|
|
|
|
4.
洪星. 生命早期双酚A暴露对子代成年小鼠焦虑和抑郁行为的影响及其神经机制.
心理学报,2012,44(9):1167-1179
|
CSCD被引
3
次
|
|
|
|
|
5.
熊航. 五志化火与炎症因子相关性的思考.
北京中医药大学学报,2012,35(2):77-79,84
|
CSCD被引
2
次
|
|
|
|
|
6.
Connor T J. Depression,stress and immunological activation:The role of cytokines in depressive disorders.
Life Sci,1998,62(7):583-606
|
CSCD被引
7
次
|
|
|
|
|
7.
Fiore M. Exploratory and displacement behavior in transgenic mice expressing high levels of brain TNFalpha.
Physiol Behav,1998,63(4):571-576
|
CSCD被引
1
次
|
|
|
|
|
8.
Vogelzangs N. Anxiety disorders and inflammation in a large adult cohort.
Transl Psychiatry,2013,3:e249
|
CSCD被引
4
次
|
|
|
|
|
9.
Hou Ruihua. Peripheral inflammatory cytokines and immune balance in Generalised Anxiety Disorder:Case-controlled study.
Brain Behav Immun,2017,62:212-218
|
CSCD被引
1
次
|
|
|
|
|
10.
黎杏群. 肝火上炎证的病理生理学基础研究.
中医杂志,2002,43(1):54-56
|
CSCD被引
7
次
|
|
|
|
|
11.
何汝帮. “上火”证发病机理的研究综述.
亚太传统医药,2008,4(12):144-145
|
CSCD被引
6
次
|
|
|
|
|
12.
李彦. 论七情与上火.
中华中医药杂志,2017,32(2):443-445
|
CSCD被引
3
次
|
|
|
|
|
13.
朱思睿. 情志"上火"增加"疾病易感性"的研究.
中国中药杂志,2018,43(8):1529-1535
|
CSCD被引
10
次
|
|
|
|
|
了解气象卫星更多信息，请访问