窄叶鲜卑花(Sibiraea angustata)nrDNA ITS和cpDNA trnL-F 序列分子进化特点的分析
Characteristic of Molecular Evolution of Sibiraea angustata Based on nrDNA ITS and cpDNA trnL-F Sequence Analysis

段义忠 ¹ 张得钧 ¹ 高庆波 ¹ 张发起 ¹ 李印虎 ¹ 陈世龙 ² ^*

文摘	应用PCR产物直接测序法分析了窄叶鲜卑花居群间nrDNA(核糖体DNA)ITS序列和cpDNA(叶绿体 DNA)trnL-F的碱基差异,并与cpDNA trnS-G序列和rpl20-rps12序列进行比较,从而初步研究两套植物基因组的变异速率.采用改良的CTAB法从硅胶干燥的窄叶鲜卑花叶片中提取总DNA,并对nrDNA ITS和cpDNA trnL-F区域进行扩增、纯化、测序.nrDNA ITS序列共有601 bp,有变异位点3处,变异位点百分率为0.05%,(G+C)含量为 41.4%.cpDNA trnL-F序列共有927 bp,有变异位点1处,变异位点百分率0.01%,(G+C)含量为32.6%,两种序列的核苷酸多样性非常低.比较发现,窄叶鲜卑花nrDNA ITS区域较cpDNA trnS-G序列和rpl20-rps12序列保守, 变异速率较慢,比cpDNA trnL-F序列变异速率稍快.通过对ITS序列单倍型(haplotype)进行分析发现,窄叶鲜卑花现有分布范围经历了居群近期范围扩张,与叶绿体基因组(tins-G和rpl20-rpsl2序列)得出的结论一致.因此, 窄叶鲜卑花nrDNA ITS序列适合该种的谱系地理学研究.
其他语种文摘	The differences between the nrDNA ITS and cpDNA tmL-F sequences in Sibiraea angustata were analyzed by PCR direct sequencing, and then compared with cpDNA trnS-G, rpl20-rps12 sequences of this species.Total DNA was extracted from silica-dried leaves of S.angustata using modified CTAB method.With the extracted DNA as template,nrDNA ITS and cpDNA trnL-F regions were amplified,then purified and sequenced.The length of nrDNA ITS sequence of S.angustata was 601 bp,of which 3 were variable sites with a percentage of0.05%,the(G+C)content Was41.4%.The length of cpDNA trnL-F sequence of S.angustata was 927 bp,of which only one was variable site with a percentage of 0.01%,the(G+C)content Was 32.6%.The nrDNA ITS region of S.angustata Was more conserved and evolved more slowly than the cpDNA trnS-G and rpl20-rps12 sequences,but evolved a little more rapidly when compared to cpDNA trnL-F.The present distribution range of S.angustata has experienced range expansion by the haplotype analysis of this species,which consists with the conclusion resulting from cpDNA genome.Therefore.the nrDNA ITS sequence of S.angustata was fit to the phylogeographic study of this species.
来源	植物研究 ,2010,30(2):146-151 【核心库】
关键词	窄叶鲜卑花 ; ITS序列 ; trnL-F序列 ; trnS-G序列 ; rpl20-rps12序列
地址	1. 中国科学院,西北高原生物研究所, 中国科学院,高原生物适应与进化重点实验室, 青海, 西宁, 810001 2. 中国科学院,西北高原生物研究所, 高原生物适应与进化重点实验室, 青海, 西宁, 810001
语种	中文
文献类型	研究性论文
ISSN	1673-5102
学科	植物学
基金	国家自然科学基金
文献收藏号	CSCD:3835857