文摘
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目的:研究藏木香中总黄酮的提取工艺及含量测定。方法:采用正交实验进行优选,考察乙醇浓度、溶剂倍数、提取时间等因素对黄酮得率的影响,用紫外分光光度法测定其含量。结果:乙醇浓度是主要的影响因素,最佳提取工艺为选用75%乙醇作为提取溶剂,料液比1:20,水浴热回流提取2h。以芦丁为对照品,采用分光光度法测定出总黄酮平均含量为0.28 mg/g。结论:提取工艺可行;紫外分光光度法简单、快速、准确;为藏木香深度开发提供了科学依据。 |
其他语种文摘
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Objective:Aim to study the best conditions of extraction techniques for total flavonoids in Inula racemosa.Methods:The best conditions of extraction technique for total flavonoids were optimized with orthogonal experiment.Developed by ethanol solution with different concentrations,solvent multiples and extraction duration,determination of total flavonoids with UV- spectrophotometry was made.Results:Ethanol concentration was the major factor for determining total flavonoids contents.The best conditions for the extraction techniques of total flavonoids were:75% ethanol which was 20 times of the medicine amount,reflux extracting 2 hours.The yield of total flavonoids in Inula racemosa was estimated at 0.28mg/g.Conclusion:The extraction techniques are feasible and the UV-spectrophotometric method is simple,quick and accurate. |
来源
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食品工业科技
,2011,32(2):251-253 【核心库】
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关键词
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藏木香
;
黄酮
;
提取工艺
;
紫外分光光度法
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地址
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1.
中国科学院西北高原生物研究所, 青海, 西宁, 810008
2.
青海省大通宝库林场, 青海, 大通, 810100
3.
河南科技大学, 河南, 洛阳, 471003
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语种
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中文 |
文献类型
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研究性论文 |
ISSN
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1002-0306 |
学科
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轻工业、手工业、生活服务业 |
基金
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河南科技大学特聘教授科研启动费
;
青海省重点攻关项目
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文献收藏号
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CSCD:4262438
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