Effects of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis
查看参考文献37篇
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文摘
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Objective:To investigate the protective effects and mechanisms of action of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis (SAP). Methods:The rats were divided into sham-operated, model control, dexamethasone treated, and Salvia miltiorrhiza treated groups. At 3, 6, and 12 h after operation, the mortality rate of different groups, pathological changes, Bcl-2-associated X protein (Bax) and nuclear factor-κB (NF-κB) protein expression levels in multiple organs (the pancreas, liver, kidneys, and lungs), toll-like receptor 4 (TLR-4) protein levels (only in the liver), intercellular adhesion molecule 1 (ICAM-1) protein levels (only in the lung), and terminal deoxynucleotidy transferase mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) staining expression levels, as well as the serum contents of amylase, glutamate-pyruvate transaminase (GPT), glutamic-oxaloacetic transaminase (GOT), blood urea nitrogen (BUN), and creatinine (CREA) were observed. Results:The mortality rate of the dexamethasone treated group was significantly lower than that of the model control group (P<0.05). The pathological changes in multiple organs in the two treated groups were relieved to different degrees (P<0.05 and P<0.01, respectively), the expression levels of Bax and NF-κB proteins, and apoptotic indexes of multiple organs were reduced (P<0.05 and P<0.01, respectively). The contents of amylase, GPT, GOT, BUN, and CREA in the two treated groups were significantly lower than those in model control groups (P<0.05 and P<0.01, respectively). The expression level of ICAM-1 protein in the lungs (at 3 and 12 h) in the dexamethasone treated group was significantly lower than that in the Salvia miltiorrhiza treated group (P<0.05). The serum contents of CREA (at 12 h) and BUN (at 6 h) of the Salvia miltiorrhiza treated group were significantly lower than those in the dexamethasone treated group (P<0.05). Conclusions:Both dexamethasone and Salvia miltiorrhiza can reduce the inflammatory reaction, regulate apoptosis, and thus protect multiple organs of rats with SAP. |
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来源
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Journal of Zhejiang University. Science B
, Biomedicine & Biotechnology,2012,13(11):919-931 【核心库】
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DOI
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10.1631/jzus.b1100351
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关键词
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Dexamethasone
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Salvia miltiorrhiza
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Pancreatitis
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Multiple organs
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Rats
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Apoptosis
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NF-κB
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TLR-4
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ICAM-1
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Tissue microarrays
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地址
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1.
Department of General Surgery, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200092
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Department of Breast Surgery, Zhejiang Cancer Hospital, Hangzhou, 310022
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Department of Orthopaedics, Jiaxing Hospital of Traditional Chinese Medicine, Jiaxing, 314001
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Zhejiang Chinese Medical University, Hangzhou, 310053
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语种
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英文 |
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ISSN
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1673-1581 |
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学科
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内科学 |
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基金
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by the Foundation for the Excellent Middle-Aged and Talented Young Persons of Zhejiang Province“151”, China
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文献收藏号
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CSCD:4674772
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