文摘
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目的采用高效液相色谱法研究并建立牦牛皮胶氨基酸类成分的指纹图谱,为牦牛皮胶的质量控制提供有效的方法。方法采用柱前衍生-RP高效液相色谱法,梯度洗脱进行分离,固定相为Agilent Analytical XDB-C_(18),(4.6 mm×250mm,5μm);流动相为pH6.4的醋酸钠缓冲液(A)-60%乙腈(B),检测波长为360 nm,体积流量为1.0 mL/min,分析时间为48 min。结果10批样品具有较高的相似度。天冬氨酸、谷氨酸、丝氨酸、甘氨酸、苏氨酸、脯氨酸、丙氨酸、缬氨酸、甲硫氨酸、异亮氨酸、亮氨酸、苯丙氨酸和赖氨酸的平均质量分数分别为5.58%、10.49%、3.35%、23.25%、1.74%、14.19%、8.98%、2.48%、0.29%、1.6%、3.07%、2.12%和3.57%。结论该方法稳定可靠,可为其氨基酸质量的全面控制提供参考。 |
其他语种文摘
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AIM To develop an HPLC method for controlling the quality of yak collagen according to the amino acids constituents.METHODS The amino acids constituents were analysed by Pre-column derivatization reversed phase high performance liquid chromatography.Chromatographic conditions were as follows: Agilent Analytical XDB-C_(18) column(4.6 mm×250 mm,5 μm),mobile phase was natrium aceticum buffer solution(A)(pH = 6.5)-60%acetonitrie(B),the detective wavelength was set at 360 nm,the flow rate was 1 mL /min and the analytical time was 48 minutes.RESULTS The result showed that the amino acids constituents had a high similarity.The contents of Asp,Glu,Ser,Gly,Thr,Arg,Ala,Val,Met,Ile,Leu,Phe and Lys were 5.58%,10.49%,3.35%,23.25%,1.74%,14.19%,8.98%,2.48%,0.29%,1.6%,3.07%,2.12% and 3.57%,respectively.CONCLUSION The method has good reproducibility and stability,and can be served as the means to control the quality of yak collagen. |
来源
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中成药
,2012,34(2):195-198 【核心库】
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关键词
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牦牛皮胶
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氨基酸
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高效液相
;
指纹图谱
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地址
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中国科学院西北高原生物研究所藏药现代化研究中心, 青海省藏药药理学与安全性评价研究重点实验室, 青海, 西宁, 810008
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语种
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中文 |
文献类型
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研究性论文 |
ISSN
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1001-1528 |
学科
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中国医学 |
基金
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青海省科技厅院士基金
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文献收藏号
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CSCD:4453131
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