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文摘
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[目的]探究青海家牦牛HIF-1α基因组织的特异性表达。[方法]应用半定量反转录PCR和实时定量反转录PCR（SYBRGreen）技术对青海家牦牛HIF-1α基因的组织特异性表达进行检测。通过提取不同组织总RNA，经DNase I消化后，用随机引物进行反转录合成cDNA，采用特异性引物分别对HIF-1α和β-actin基因进行RT-PCR和Real Time RT-PCR扩增。[结果]结果表明，HIF-1α基因在心、肝、脾、肺、肾、脑、肌肉、睾丸组织中均有表达，其中以睾丸和脾中HIF-1α基因表达量最高，肌肉的表达量最低。[结论]该研究为进一步揭示HIF-1α在高原土著动物低氧适应过程中的分子机制有着重要的意义。 |
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其他语种文摘
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[Objective] The experiment was conducted to study the tissue specific expression of HIF-1α gene in Qinghai domestic yak. [Method] Tissue specific expression of HIF-1α gene in Qinghai domestic yak was detected using the methods of Semi-quantitative RT-PCR and Real Time RT-PCR. RNA was extracted from different tissues. After treated with DNase I, the total RNA was reverse transcribed with random primer to synthesize cDNA. Then the HIF-1α gene and β-actin gene were amplified with specific primers by RT-PCR and Real Time RT-PCR. [Result] Result showed that HIF-1α gene was expressed in tissues including heart, liver, spleen, lung, kidney, brain, muscle and testis. Among them,HIF-1α gene showed the highest expression amount in testis and spleen and lowest in muscle. [Conclusion] This study was of significant importance for the study on the molecular mechanism of HIF-1α in plateau native animals during the process of hypoxia adaptation. |
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来源
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安徽农业科学
,2007,35(29):9173-9175 【扩展库】
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关键词
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青海家牦牛
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HIF-1
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α半定量反转录PCR
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实时定量反转录PCR
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组织分布
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地址
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中国科学院西北高原生物研究所, 青海, 西宁, 810001
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0517-6611 |
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学科
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分子生物学 |
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基金
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国家973计划
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文献收藏号
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CSCD:3053930
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