一种大豆对拟茎点茎枯病抗性鉴定技术体系的构建
Establishment of a technical system for the identification of soybean resistance to Phomopsis stem blight
查看参考文献26篇
|
文摘
|
由Phomopsis longicolla(或Diaporthe longicolla)等拟茎点霉(间座壳)属真菌引起的拟茎点茎枯病是我国黄淮海等地大豆生产中的一种重要茎部病害。利用抗病品种是防控该病最为经济有效的措施。为构建一种大豆对拟茎点茎枯病的室内抗性鉴定技术体系,用于抗病资源的快速筛选,本研究比较了下胚轴创伤接种法、切茎接种法、黄化苗接种法、种子接种法和菌丝块贴茎法等5种接种方法,发现下胚轴创伤接种法具有操作简单、试验周期短、易于判定结果、结果稳定性好的优点。选取5个具有毒力强弱和地域代表性的P. longicolla菌株为鉴别菌株系,建立了抗性结果评价标准,形成了抗性鉴定技术体系。对2020年从黄淮海等地收集的62份大豆主栽品种进行抗性鉴定,发现对强毒力菌株DT3-3-1和弱毒力菌株ZZ1-1高抗的品种分别占31%和68%,有13个品种同时高抗5个菌株(占21%),表明我国大豆主栽品种中存在对拟茎点茎枯病的抗性种质资源。本研究为挖掘大豆对拟茎点茎枯病的抗性资源提供了新的候选方法。 |
|
其他语种文摘
|
The occurrence of Phomopsis stem blight, caused by Phomopsis longicolla(syn. Diaporthe longicolla)and other Diaporthe/ Phomopsis genus fungi, is one of the most serious soybean stem diseases in HuangHuai-Hai and other soybean-production areas of China. Using resistance varieties is the most economical and effective method to control the disease. To establish a technical system for the indoor and rapid identification of soybean resistance to Phomopsis stem blight, and apply it to identify the disease-resistant germplasm resources of soybean, we compared five inoculation methods, including hypocotyl inoculation with wounding, cut-seedling inoculation, etiolated hypocotyl inoculation, mycelium inoculation on seed, and mycelium inoculation on stem. We found that the hypocotyl inoculation with wounding method has the advantages of simple operation, short test period, easy to determine the results, and good stability. In addition, five representative P. longicolla strains with different virulence levels and from different areas were selected and the evaluation criteria of resistance identification results were established, making up a complete set of resistance identification technical system. The system was used to identify 62 popular soybean varieties collected in 2020 from Huang-Huai-Hai and other regions. Among these soybean varieties, 31% and 68% of the varieties showed high resistance to the strong virulence strain DT3-3-1 and the weak virulence strain ZZ1-1, respectively; and 13(21%)varieties were highly resistant to all five P. longicolla strains. The results revealed that there are soybean germplasm resources resistant to Phomopsis stem blight in China. The technical system established in this study provides a new candidate method for mining the soybean germplasm resources which are resistant to Phomopsis stem blight. |
|
来源
|
植物病理学报
,2022,52(6):959-966 【核心库】
|
|
DOI
|
10.13926/j.cnki.apps.000605
|
|
关键词
|
间座壳/拟茎点霉
;
大豆茎部病害
;
抗病性鉴定
;
抗病品种
|
|
地址
|
1.
南京农业大学植物保护学院, 南京, 210095
2.
济宁市农业科学研究院, 济宁, 272131
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
0412-0914 |
|
学科
|
植物保护 |
|
基金
|
财政部和农业农村部-国家现代农业产业技术体系专项
|
|
文献收藏号
|
CSCD:7415490
|
参考文献 共
26
共2页
|
|
1.
Hobbs T W. A new Phomopsis species from soybean.
Mycologia,1985,77(4):535-544
|
CSCD被引
7
次
|
|
|
|
|
2.
Cui Y L. First report of Phomopsis longicolla causing soybean stem blight in China.
Plant Pathology,2009,58(4):799
|
CSCD被引
3
次
|
|
|
|
|
3.
Mena E. Soybean stem canker caused by Diaporthe caulivora; pathogen diversity, colonization process, and plant defense activation.
Frontiers in Plant Science,2019,10:1733
|
CSCD被引
1
次
|
|
|
|
|
4.
Santos J M. Resolving the Diaporthe species occurring on soybean in Croatia.
Persoonia,2011,27:9-19
|
CSCD被引
7
次
|
|
|
|
|
5.
Mathew F M. Identification of Diaporthe longicolla on dry edible pea, dry edible bean, and soybean in North Dakota.
Plant Health Progress,2015,16(2):71-72
|
CSCD被引
1
次
|
|
|
|
|
6.
Yang Q. High diversity of Diaporthe species associated with dieback diseases in China, with twelve new species described.
MycoKeys,2018(39):97-149
|
CSCD被引
2
次
|
|
|
|
|
7.
Manawasinghe I S. High genetic diversity and species complexity of Diaporthe associated with grapevine dieback in China.
Frontiers in Microbiology,2019,10:1936
|
CSCD被引
3
次
|
|
|
|
|
8.
Ye W. A LAMP-assay-based specific microbiota analysis reveals community dynamics and potential interactions of 13 major soybean root pathogens.
Journal of Integrative Agriculture,2020,19(8):2056-2063
|
CSCD被引
2
次
|
|
|
|
|
9.
Sinclair J B. Phomopsis seed decay of soybeans-a prototype for studying seed disease.
Plant Disease,1993,77(4):329-334
|
CSCD被引
1
次
|
|
|
|
|
10.
Pioli N. Morphologic, molecular, and pathogenic characterization of Diaporthe phaseolorum variability in the core soybean-producing area of Argentina.
Phytopathology,2003,93(2):136-146
|
CSCD被引
1
次
|
|
|
|
|
11.
Sun X. First report of Diaporthe longicolla causing leaf spot on Kalanchoe pinnata in China.
Plant Disease,2021,105(11):3739
|
CSCD被引
1
次
|
|
|
|
|
12.
Zhu X. Identification of pathogen causing new disease of Prunus salicina Lindl. var. cordata Y. He et J. Y. Zhang cv. younai fruits in Yongzhou, Hunan (in Chinese).
南方农业学报,2016,47(8):1318-1325
|
CSCD被引
1
次
|
|
|
|
|
13.
Hu J. First report of Phomopsis longicolla causing stem canker of eggplant in Fujian Province, China.
Plant Disease,2021,105(10):3296
|
CSCD被引
1
次
|
|
|
|
|
14.
Shan Z. First report of Phomopsis seed decay of soybean caused by Phomopsis longicolla in south China.
Plant Disease,2012,96(11):1693
|
CSCD被引
6
次
|
|
|
|
|
15.
Xue C S. First report of Phomopsis longicolla causing leaf spot on soybean in China.
Plant Disease,2015,99(2):290
|
CSCD被引
3
次
|
|
|
|
|
16.
Ye W W. Research progress on key technologies for monitoring and control of soybean root rot (in Chinese).
大豆科学,2020,39(5):804-809
|
CSCD被引
1
次
|
|
|
|
|
17.
Tang Q H. Resistance evaluation of soybean germplasm from Huanghuai valley to Phytophthora root rot (in Chinese).
中国农业科学,2010,43(11):2246-2252
|
CSCD被引
2
次
|
|
|
|
|
18.
Yang J. Identification of soybean resistance to Phytophthora sojae in the germplasm resources from Huanghuaihai region of china (in Chinese).
大豆科学,2020,39(1):12-22
|
CSCD被引
1
次
|
|
|
|
|
19.
Li S. Evaluation of diverse soybean germplasm for resistance to Phomopsis seed decay.
Plant Disease,2015,99(11):1517-1525
|
CSCD被引
2
次
|
|
|
|
|
20.
Cui Y L.
Identification of pathogen causing soybean stem blight and potential geographic distribution of quarantine pathogens of soybean (in Chinese),2009
|
CSCD被引
1
次
|
|
|
|
|
了解气象卫星更多信息，请访问