引起莴苣灰霉病的灰葡萄孢红色菌系的分离及鉴定
Isolation and identification of a red strain of Botrytis cinerea causing gray mold on lettuce
查看参考文献10篇
|
文摘
|
灰葡萄孢(Botrytis cinerea)是一种死体营养型植物病原真菌,寄主范围广泛,可以侵染1 400多种植物,其中包括葡萄、番茄、草莓、莴苣等具重要经济价值的作物。前人根据灰葡萄孢分生孢子的产生和分布以及菌核的有无,将形态表型分为菌丝型、孢子型和菌核型。科研人员还根据灰葡萄孢菌核的颜色将其分类为黑色菌系、黄色菌系和黄黑色菌系(菌核开始为黄色,最终变为黑色)。2021年4月,课题组在湖北武汉调查莴苣病害发生情况时观察到一株疑似能够产生红色分泌物的病原菌。经单孢分离、形态学观察、致病力测定以及分子生物学鉴定,明确了其分类学地位。本研究旨在拓展人们对于灰葡萄孢遗传多样性的认识,并为灰霉病的综合防治提供参考。 |
|
其他语种文摘
|
Gray mold,caused by the necrotrophic fungal pathogen Botrytis cinerea,is one of the most devastating diseases on lettuce.In this study,tissue isolation was performed to obtain a pure culture that caused necrotic symptoms on lettuce.In order to identify the pathogen,single spore isolation,morphological observation,pathogenicity determination,sequence-characterized amplified region (SCAR) marker detection,and phylogenetic analysis were conducted.The results showed that this pathogen appeared red when grown on potato dextrose agar (PDA) plates and was identified as B.cinerea.This is the first report of a red strain of B.cinerea. |
|
来源
|
植物病理学报
,2023,53(5):966-969 【核心库】
|
|
DOI
|
10.13926/j.cnki.apps.000830
|
|
关键词
|
生菜灰霉菌
;
灰葡萄孢
;
红色菌株
;
生物学鉴定
|
|
地址
|
1.
武汉市农业科学院, 武汉, 430065
2.
华中农业大学, 武汉, 430070
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
0412-0914 |
|
学科
|
植物保护 |
|
基金
|
财政部和农业农村部:国家现代农业产业技术体系资助
;
湖北省博士后资助项目
;
武汉市农业科学院创新体系建设青年创新项目
|
|
文献收藏号
|
CSCD:7616502
|
参考文献 共
10
共1页
|
|
1.
Elad Y.
Botrytis-the fungus, the pathogen and its management in agricultural systems,2016:413-486
|
CSCD被引
6
次
|
|
|
|
|
2.
Zhang Y J. Research advances in the diversity of Botrytis cinerea (in Chinese).
农业生物技术学报,2017,25(6):954-968
|
CSCD被引
2
次
|
|
|
|
|
3.
Li N.
Studies on diversity of Botrytis cinerea isolated from tomato and strawberry (in Chinese),2015
|
CSCD被引
1
次
|
|
|
|
|
4.
Fang Z D.
Research method for plant pathology (in Chinese),2017
|
CSCD被引
1
次
|
|
|
|
|
5.
Li T G. Study on growth and sporulation conditions of grape Botrytis cinerea (in Chinese).
中外葡萄与葡萄酒,2021(2):6-11
|
CSCD被引
1
次
|
|
|
|
|
6.
Wu F H. Comparing study on several methods for DNA extraction from endophytic fungi (in Chinese).
中国农学通报,2009,25(8):62-64
|
CSCD被引
3
次
|
|
|
|
|
7.
Fan X.
Studies on molecular detection and identification of Botrytis species infecting major fruits and vegetables (in Chinese),2015
|
CSCD被引
2
次
|
|
|
|
|
8.
Zahng J.
Studies on taxonomy of Botrytis species in Hubei province and diversity of B. Cinerea (in Chinese),2010
|
CSCD被引
1
次
|
|
|
|
|
9.
Ma W S. Gray mold disease of Paris polyphylla var. yunnanensis and its pathogen identification (in Chinese).
中国中药杂志,2018,43(14):2918-2927
|
CSCD被引
2
次
|
|
|
|
|
10.
Han Z Q. Research progress on Botrytis cinerea resistance to different fungicides (in Chinese).
中国蔬菜,2014(5):6-10
|
CSCD被引
1
次
|
|
|
|
|
了解气象卫星更多信息，请访问