Heterodera filipjevi许昌群体SCAR标记的建立
Development of SCAR markers related to Heterodera filipjevi Xuchang population
查看参考文献42篇
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文摘
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菲利普孢囊线虫Heterodera filipjevi是禾谷类作物上重要的病原线虫之一,严重影响禾谷类作物的产量和品质.本课题组前期研究发现菲利普孢囊线虫许昌群体是一个新的致病型,其在小麦上的致病力强于其他多个群体,危害更为严重.本研究旨在建立菲利普孢囊线虫许昌群体的快速、准确的分子检测体系,为Heterodera filipjevi许昌群体的监测和防控及抗病品种的选育利用奠定基础.本研究采用随机扩增多态性DNA(RAPD)和序列特征扩增区域(SCAR)的方法,对黄淮麦区5个重要小麦孢囊线虫致病型共9个线虫群体进行RAPD分析和SCAR标记转化,并通过增加除黄淮麦区外的线虫群体验证所获得的致病型相关分子标记的特异性和有效性.本研究共筛选了331条RAPD引物,筛选出2个菲利普孢囊线虫许昌群体相关的RAPD标记,引物S86可以扩增出1条约550 bp的多态性片段,引物S178可以扩增出1条约1 200 bp的多态性片段,并将这2个RAPD标记成功转化为SCAR标记.SCAR标记的特异性检测结果表明这两个SCAR标记只在许昌群体上有特异性扩增,可以用于许昌群体的分子检测. |
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其他语种文摘
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Heterodera filipjevi is one of the important pathogenic nematodes that endanger the cereal crops in temperate zone,and it caused serious influence of the yield and quality. H. filipjevi is a newly identified species of cereal cyst nematode in central China. More than three years studies showed that Xuchang population is a new pathotype of H. filipjevi,and its pathogenicity was stronger than other populations of cereal cyst nematode in Huang-Huai Plain. This study aims to establish a rapid and accurate molecular detection system of Xuchang population,lay the foundation for monitoring and controlling Xuchang population of H. filipjevi and provide the basis for breeding and utilization of resistant varieties. This study focused on using RAPD-SCAR technique to analyze 9 CCN populations of 5 different CCN pathotpyes from Huang-Huai Plain. Moreover,8 extra CCN populations were added to verify the specificity and validity of SCAR markers. 331 random primers were screened and two random primers with RAPD method were found related to Xuchang population,Primer S86, S178 could product polymorphic bands of Xuchang population about 550 bp and 1 200 bp respectively. The two RAPD markers related to Xuchang population of H. filipjevi had been transformed into SCAR markers successfully. The results of the specific detection showed that the two SCAR markers can detect the Xuchang population from other CCN populations in Huang-huai floodplain. The two SCAR markers can be used to detect and identify H. filipjevi Xuchang population. |
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来源
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植物病理学报
,2018,48(2):239-247 【核心库】
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DOI
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10.13926/j.cnki.apps.000206
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关键词
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菲利普孢囊线虫
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许昌群体
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SCAR标记
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地址
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河南农业大学植物保护学院, 河南省粮食作物协同创新中心, 郑州, 450002
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0412-0914 |
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学科
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植物保护 |
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基金
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国家自然科学基金资助项目
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文献收藏号
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CSCD:6220428
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参考文献 共
42
共3页
|
|
1.
Rivoal R. Past research on the cereal cyst nematode complex and future needs.
Cereal Cyst Nematodes: Status,Research and Outlook,2009:3-10
|
CSCD被引
4
次
|
|
|
|
|
2.
Chen P S. Wheat cereal cyst nematode disease(in Chinese).
植物保护,1992,18(6):37-38
|
CSCD被引
3
次
|
|
|
|
|
3.
Wang Z Y. A preliminary study on wheat cyst nematode disease (Heterodera avenae Wollenweber) in Henan Province (in Chinese).
华北农学报,1993,8(suppl):105-109
|
CSCD被引
2
次
|
|
|
|
|
4.
Yang Y Y. New distribution report of cereal cyst nematode on wheat in Shandong province (in Chinese).
青岛农业大学学报,2010,27(1):17-20
|
CSCD被引
2
次
|
|
|
|
|
5.
Chen X. Distribution of Heterodera avenae in the middle and west regions of Inner Mongolia,China (in Chinese).
植物保护,2009,35(5):114-117
|
CSCD被引
3
次
|
|
|
|
|
6.
Hou S Y. First report of wheat cyst nematode in Qinghai province (in Chinese).
青海大学学报,2008,26(5):84-86
|
CSCD被引
1
次
|
|
|
|
|
7.
Yang C G. Investigations on the distribution of and damages caused by cereal cyst nematode in Anhui (in Chinese).
植物保护,2008,34(2):107-110
|
CSCD被引
2
次
|
|
|
|
|
8.
Peng D L. First report of cereal cyst nematode (Heterodera avenae) in Tianjin, China.
Nematology Research in China,2012:162-163
|
CSCD被引
1
次
|
|
|
|
|
9.
Li H X. The detection of Heterodera avenae from the cereal field in Autonomous Regions of Tibet and Xinjiang.
Nematology Research in China (in Chinese),2012:164-165
|
CSCD被引
1
次
|
|
|
|
|
10.
Lv Y. A survey of wheat yield loss caused by cereal cyst nematode (in Chinese).
河南农业,2009,20(6):20
|
CSCD被引
1
次
|
|
|
|
|
11.
Stone A. Some problems posed by the Heterodera avenae complex.
EPPO Bulletin,1982,12(4):317-320
|
CSCD被引
1
次
|
|
|
|
|
12.
Handoo Z A. A key and compendium to species of the Heterodera avenae group (Nematoda: Heteroderidae).
Journal of Nematology,2002,34(3):250-262
|
CSCD被引
21
次
|
|
|
|
|
13.
Abidou H. Occurrence and distribution of species of the Heterodera avenae group in Syria and Turkey.
Nematologia Mediterranea,2005,33(2):195-201
|
CSCD被引
3
次
|
|
|
|
|
14.
Toumi F. Distribution of cereal cyst nematodes (Heterodera spp.) in wheat and barley fields in north-eastern regions of Syria.
Journal of Plant Diseases and Protection,2015,122(5):255-263
|
CSCD被引
1
次
|
|
|
|
|
15.
Rivoal R.
Plant parasitic nematodes in temperate agriculture,1993:259-303
|
CSCD被引
16
次
|
|
|
|
|
16.
Madzhidov A R. Bidera filipjevi n. sp. (Heteroderina: Tylenchida) in Tadzhikistan.
Biologicheskie Nauki,1981,24(2):40-44
|
CSCD被引
1
次
|
|
|
|
|
17.
Peng D L. First report of the cyst nematode (Heterodera filipjevi) on wheat in Henan province,China.
Plant Disease,2010,94(10):1262
|
CSCD被引
30
次
|
|
|
|
|
18.
Li H L. First record of the cereal cyst nematode Heterodera filipjevi in China.
Plant Disease,2010,94(12):1505
|
CSCD被引
19
次
|
|
|
|
|
19.
Nian G L. Identification on the pathotypes of three Heterodera filipjevi populations from Henan Province.
Nematology Research in China (in Chinese),2010:130-133
|
CSCD被引
1
次
|
|
|
|
|
20.
Semblat J P. Highresolution DNA fingerprinting of parthenogenetic rootknot nematodes using AFLP analysis.
Molecular Ecology,1998,7(1):119-125
|
CSCD被引
5
次
|
|
|
|
|
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