帮助 关于我们

返回检索结果

小球藻在蔗渣酶解液中的异养生长及其脂肪酸生成
Cell growth and fatty acid production of heterotrophic microalgae Chlorella sp. cultivated in enzymatic hydrolyzate of sugarcane bagasse

查看参考文献26篇

王闻 1   杨康 1   朱顺妮 1   冯佳 1   尚常花 1   王忠铭 1   袁振宏 2 *   庄新姝 1   胡磊 3  
文摘 在高温液态水处理的甘蔗渣酶解过程中添加Tween80可使聚糖转化率提高11.4%。根据蔗渣酶解液中糖的种类及含量,用葡萄糖、木糖和纤维二糖标准品模拟蔗渣酶解液组成配制成相应的混合糖培养基,同时配制仅含葡萄糖的培养基,在有、无Tween80和BG11(Blue-Green 11)的条件下,考察小球藻在不同培养基中的异养生长及脂肪酸生成。结果显示Tween80对小球藻的生长具有抑制作用,纤维二糖也会影响小球藻的生长;小球藻在添加BG11的葡萄糖培养基中的生物量最高,为1.97 g·L~(-1),在添加BG11的蔗渣酶解液中的生物量高出未添加BG11的2倍,在含有Tween80和BG11的蔗渣酶解液中的总脂肪酸含量最高,达到6.90%,在所有培养基中产生的脂肪酸以C_(16:0)、C_(18:1)、C_(18:3)、C_(20:1)和C_(20:4)为主;培养基组成优化可进一步提高微藻生物量和油脂产量。
其他语种文摘 After adding Tween80 into the enzymatic hydrolysis process, the glycan conversion of sugarcane bagasse (SCB) pretreated by liquid hot water (LHW) was improved 11.4%. The enzymatic hydrolyzates with and without Tween80 were prepared as media to cultivate heterotrophic Chlorella sp.. According to the compositional feature of enzymatic hydrolyzate, the synthetic media composed of glucose, xylose and cellobiose were prepared to mimic the media containing enzymatic hydrolyzate. The medium which only contained glucose was used as the positive control. The glucose concentration in all of the media was 10 g·L~(-1). The synthetic media containing Tween80 or not were mixed with BG11 which did not contain carbonate, while the hydrolyzates media were prepared with or without the foregoing BG11. The cell growth and fatty acids yield of heterotrophic Chlorella sp. cultivated on the above media were investigated. The results showed that Tween80 could inhibit the cell growth of Chlorella sp., and cellobiose could also put negative impact on the biomass of Chlorella sp.. Chlorella sp. cultivated on medium containing glucose and BG11 attained the maximum biomass of 1.97 g·L~(-1). The biomass of Chlorella sp. growing on enzymatic hydrolyzate media with BG11 was 2 times more than that on enzymatic hydrolyzate media without BG11.6.90% of maximum fatty acid content was achieved when Chlorella sp. was cultured in enzymatic hydrolyzate media containing Tween80 and BG11. The chief fatty acids produced in all media were C_(16:0), C_(18:1), C_(18:3), C_(20:1) and C_(20:4). The addition of inorganic and organic nutrients into cellulolytic hydrolyzate can enhance the biomass and lipid content of microalgae.
来源 化工学报 ,2016,67(4):1549-1556 【核心库】
DOI 10.11949/j.issn.0438-1157.20150977
关键词 微藻 ; 生物质 ; 生物催化 ; 异养培养 ; 生物柴油 ; 高温液态水
地址

1. 中国科学院广州能源研究所, 中国科学院可再生能源重点实验室;;广东省新能源和可再生能源研究开发与应用重点实验室, 广东, 广州, 510640  

2. 中国科学院广州能源研究所, 中国科学院可再生能源重点实验室;;广东省新能源和可再生能源研究开发与应用重点实验室;;生物质能源河南省协同创新中心, 广东, 广州, 510640  

3. 淮阴师范学院, 江苏省生物质能与酶技术重点实验室, 江苏, 淮安, 223300

语种 中文
文献类型 研究性论文
ISSN 0438-1157
学科 能源与动力工程
基金 国家教育部留学回国人员科研启动基金 ;  江苏省生物质能与酶技术重点实验室开放课题基金 ;  国家自然科学基金项目 ;  国家973计划
文献收藏号 CSCD:5677199

参考文献 共 26 共2页

1.  Kim D G. Growth and fatty acid composition of three heterotrophic Chlorella species. Algae,2013,28(1):101-109 CSCD被引 1 次    
2.  Miao X. Biodiesel production from heterotrophic microalgal oil. Bioresoure Technology,2006,97:841-846 CSCD被引 22 次    
3.  Li X. Large-scale biodiesel production from microalga Chlorella protothecoides through heterotrophic cultivation in bioreactors. Biotechnology and Bioengineering,2007,98:761-771 CSCD被引 1 次    
4.  Wei A. Effects of cassava starch hydrolysate on cell growth and lipid accumulation of the heterotrophic microalgae Chlorella protothecoides. Journal of Industrial Microbiology & Biotechnology,2009,36:1383-1389 CSCD被引 7 次    
5.  Huo S H. Cultivation of Chlorella zofingiensis in bench-scale outdoor ponds by regulation of pH using dairy wastewater in winter, South China. Bioresource Technology,2012,121:76-82 CSCD被引 2 次    
6.  Gao C. Application of sweet sorghum for biodiesel production by heterotrophic microalga Chlorella protothecoides. Applied Energy,2010,87:756-761 CSCD被引 3 次    
7.  Espinosa-Gonzalez I. Heterotrophic growth and lipid accumulation of Chlorella protothecoides in whey permeate, a dairy by-product stream, for biofuel production. Bioresource Technology,2014,155:170-176 CSCD被引 1 次    
8.  Mu J. Enhanced biomass and oil production from sugarcane bagasse hydrolysate (SBH) by heterotrophic oleaginous microalga Chlorella protothecoides. Bioresource Technology,2015,185:99-105 CSCD被引 1 次    
9.  Gui X. Fungus-assisted mild acid pretreatment of Glycyrrhiza uralensis residues to enhance enzymatic hydrolysis and oil production by green microalga Chlorella protothecoides. Industrial Crops and Products,2014,62:466-473 CSCD被引 1 次    
10.  Malherbe S. Lignocellulose biodegradation: fundamentals and applications. Reviews in Environmental Science and Biotechnology,2002,1:105-114 CSCD被引 19 次    
11.  Miazek K. Effect of lignocellulose related compounds on microalgae growth and product biosynthesis: a review. Energies,2014,7:4446-4481 CSCD被引 4 次    
12.  Alvira P. Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. Bioresource Technology,2010,101:4851-4861 CSCD被引 116 次    
13.  Garrote G. Hydrothermal processing of lignocellulosic materials. Holz als Roh- und Werkstoff,1999,57:191-202 CSCD被引 27 次    
14.  Wang W. High consistency enzymatic saccharification of sweet sorghum bagasse pretreated with liquid hot water. Bioresource Technology,2012,108:252-257 CSCD被引 5 次    
15.  Ghose T K. Measurement of cellulose activities. Pure & Applied Chemistry,1987,59:257-268 CSCD被引 425 次    
16.  Sluiter A. Determination of structural carbohydrates and lignin in biomass: Technical Report NREL/TP-510-42618,2008 CSCD被引 1 次    
17.  Wang W. Effect of structural changes on enzymatic hydrolysis of eucalyptus, sweet sorghum bagasse, and sugarcane bagasse after liquid hot water pretreatment. Bioresources,2012,7(2):2469-2482 CSCD被引 2 次    
18.  黄伟. 氮缺乏条件下小球藻碳水化合物与脂肪酸的合成规律研究. 太阳能学报,2014,35(12):2559-2564 CSCD被引 5 次    
19.  Wang W. Highly efficient conversion of sugarcane bagasse pretreated with liquid hot water into ethanol at high solid loading. International Journal of Green Energy,2014 CSCD被引 1 次    
20.  王闻. 离子及表面活性剂对甜高粱秆渣酶解的影响. 化工学报,2013,64:3767-3774 CSCD被引 2 次    
引证文献 1 篇

1 王铭 海带渣资源化利用及在小球藻培养中的应用研究 海洋与湖沼,2022,53(1):96-105
CSCD被引 0 次

显示所有1篇文献

论文科学数据集
PlumX Metrics
相关文献

 作者相关
 关键词相关
 参考文献相关

版权所有 ©2008 中国科学院文献情报中心 制作维护：中国科学院文献情报中心
地址：北京中关村北四环西路33号 邮政编码：100190 联系电话：(010)82627496 E-mail:cscd@mail.las.ac.cn 京ICP备05002861号-4 | 京公网安备11010802043238号