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产挥发性物质芽孢杆菌对芒果炭疽菌的抑制作用及对芒果炭疽病的防病效果
Inhibitory effect of volatile substance produced by Bacillus on Colletotrichum gloesporioides and its control effect on mango anthracnose

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杨芝霓 1,2   鲁萌萌 1   黄穗萍 1   唐利华 1   陈小林 1   郭堂勋 1   莫贱友 1   张萌 3   李其利 1 *  
文摘 本研究从健康的芒果表皮分离得到10株对芒果炭疽菌具有抑菌活性的菌株,通过形态学观察、生理生化测定和分子生物学鉴定,6株菌株被鉴定为枯草芽孢杆菌(Bacillus subtilis),3株菌株被鉴定为解淀粉芽孢杆菌(B. amyloliquefaciens), 1株菌株被鉴定为贝莱斯芽孢杆菌(B. velezensis) 。采用平板对峙法和平板对扣法测定了10株芽孢杆菌的抑菌活性,结果表明,供试的10株菌株的发酵液和挥发性物质对芒果炭疽病菌均具有一定抑制作用,其中菌株N-16-2的抑制效果最好,其发酵液和挥发性物质抑菌率均达79%以上。在干燥器中测定了10株拮抗细菌产生的挥发性物质对芒果采后炭疽病的防治效果,结果表明, 10株菌株对芒果采后炭疽病的防病效果均在60%以上,其中菌株YM-11-C对采后炭疽病的防效最佳,高达70%。采用固相微萃取气质联用测定细菌产生的挥发性物质的成分,结果表明, 10株拮抗细菌挥发性物质成分主要为以下6种化合物,Diethyl Phthalate,Hexanedioic acid,bis(2-ethylhexyl) ester,Butylated Hydroxytoluene,Squalene,2-Dodecanone, 2,5 -Cyclohexadiene-1,4 -dione,2,6 -bis(1,1 -dimethylethyl) -,对这6种挥发性化合物进行抑菌活性测定,结果表明Diethyl Phthalate,2-Dodecanone和2,5 -Cyclohexadiene-1,4 -dione,2,6 -bis(1,1 -dimethylethyl)-这3种化合物具有明显抑菌活性,其中化合物2,5 -Cyclohexadiene-1,4 -dione,2,6 -bis(1,1 -dimethylethyl)-对芒果炭疽菌的抑制效果最好。本研究是首次报道枯草芽孢杆菌、解淀粉芽孢杆菌和贝莱斯芽孢杆菌产生的挥发性物质对芒果炭疽菌的抑制作用和对芒果炭疽病防病效果,也是首次报道化合物2,5 -Cyclohexadiene-1,4 -dione,2,6 -bis(1,1 -dimethylethyl)-对芒果炭疽菌的抑制作用和对芒果炭疽病防病效果。
其他语种文摘 In this study,10 bacterial strains with inhibitory activities against Colletotrichum gloesporioides from mango were isolated from the epidermis of healthy mangoes. Six strains were identified as Bacillus subtilis,three strains were identified as B. amyloliquefaciens,and one was identified as B. velezensis based on morphological observation,physiological,biochemical,and molecular analysis. The results of dual culture method and double Petri dish assay showed that bacterial suspensions and volatile compounds (VOCs) of 10 strains had inhibitory effects against C. gloesporioides,among which the strain N-16-2 showed the best inhibitory effect (>79%). The effect of VOCs produced by 10 strains on the control of post-harvest mango anthracnose was measured in glass desiccators,and the results showed that control effects of all ten strains were stronger than 60% against mango anthracnose,with strain YM-11-C showing the best control effect of 70%. Moreover,solid phase microextraction (SPME) and gas chromatography-mass spectrometry (GC-MS) were used to identify the components of the bacterial VOCs. The results showed that the VOCs of ten bacteria mainly contained six types of compounds,including phenols,esters,ketones,olefins,alkanes,quinones,and etc. Antifungal activities of six synthetic VOCs (Diethyl Phthalate,Hexanedioic acid,bis(2-ethylhexyl) ester,Butylated Hydroxytoluene,Squalene,2- Dodecanone,2,5 -Cyclohexadiene-1, 4-dione,2, 6-bis(1, 1-dimethylethyl) -) were tested,and the results showed that Diethyl Phthalate,2-Dodecanone,and 2, 5-Cyclohexadiene-1, 4-dione,2, 6-bis(1,1 -dimethylethyl) -had significant inhibitory activities,with the quinone 2,5-Cyclohexadiene-1,4-dione,2,6-bis (1,1-dimethylethyl) - showing the best inhibitory effect against mango C. gloesporioides from mango. This is the first report of inhibitory effects and control effects of VOCs produced by B. subtilis,B. amyloliquefaciens,and B. velezensis on C. gloesporioides and mango anthracnose. It is also the first report of inhibitory effects and control effects of compound 2,5 -Cyclohexadiene-1,4 -dione,2, 6-bis(1, 1-dimethylethyl) - on C. gloesporioides and mango anthracnose.
来源 植物病理学报 ,2023,53(6):1180-1191 【核心库】
DOI 10.13926/j.cnki.apps.001312
关键词 芽孢杆菌 ; 挥发性物质 ; 抑菌活性 ; 芒果炭疽病
地址

1. 广西农业科学院植物保护研究所, 农业农村部华南果蔬绿色防控重点实验室;;广西作物病虫害生物学重点实验室, 南宁, 530007  

2. 桂林市经济作物技术推广站, 桂林, 541000  

3. 广西大学农学院, 南宁, 530007

语种 中文
文献类型 研究性论文
ISSN 0412-0914
学科 植物保护
基金 财政部和农业农村部:国家现代农业产业技术体系资助
文献收藏号 CSCD:7645766

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