莲藕腐败病导致藕节组织结构变异的显微观察研究
Histopathological assessment on the lotus rhizome tissue infected by Fusarium spp. causing lotus rhizome rot
查看参考文献21篇
|
文摘
|
莲藕是我国栽培面积最大、最重要的水生蔬菜。随着种植规模的扩大及栽培制度和栽培方式的单一化,莲藕病害问题日趋严重,其中莲藕腐败病危害最大也最难防治。目前国内外对镰刀菌引起莲藕腐败病致病机理方面的研究较少,本研究基于莲藕地下茎的发病症状,从微观和超显微层面揭示病害发生后地下茎及其维管束组织的变化。石蜡组织切片观察表明,随着病程的发展,发病组织会出现越来越多的被甲苯胺蓝染成深色的坏死细胞,而且这些细胞围绕维管束出现,越靠近维管束其数量越多,分布越密集。为了进一步揭示病原菌侵染对维管束的影响,本研究又利用光学显微镜和扫描电子显微镜对病藕藕丝进行观察,结果表明,随着病变程度加深,藕丝表面原本光滑紧凑的结构变得粗糙松散,且表面附着大量瘤状凸起物以及其它可能的代谢附着物。这些变化势必影响维管束内水分和养分的疏导,最终造成植株地上部分表现失水萎蔫的症状。本研究结果揭示了病害症状发生的细胞生物学机制,并为今后抗性品种的选育提供理论依据。 |
|
其他语种文摘
|
Lotus is the most important aquatic vegetable in China and has the largest cultivation area. With the expansion of cultivation and the simplification of agricultural practices, various lotus diseases have been becoming more serious, among which lotus rhizome rot disease is the most harmful and the most difficult to control. At present, there are few research reports on the pathogenesis of Fusarium spp. causing lotus rhizome rot disease around the world. From the microscopic and ultra-microscopic levels, this study revealed the changes to the rhizome and its vascular tissues during Fusarium infection. The observation of paraffin-embedded tissue sections showed that with the development of the disease, necrotic cells stained by toluidine blue appeared around the vascular bundles, and the closer to the bundles, the more numerous necrotic cells and the more densely distributed. In order to further reveal the influence of pathogen infection on vascular bundles, brightfield optical and scanning electron microscopy were used to observe the lotus fibers in the diseased lotus rhizomes. The results showed that the smooth and compact lotus fibers became rough and dispersed with the disease tending to be more severe, and a large number of galls-like protrusions and other possible metabolic attachments were attached to the surface. These changes to the vascular bundles are likely to reduce the water and nutrient transportation, and finally the aboveground parts of the plant exhibited the symptoms of water loss and wilting. The results of this study revealed the cellular biological mechanism of the disease symptom development, and provided a basis of histology for the breeding of resistant varieties in the future. |
|
来源
|
植物病理学报
,2024,54(1):82-91 【核心库】
|
|
DOI
|
10.13926/j.cnki.apps.001023
|
|
关键词
|
莲藕腐败病
;
镰刀菌
;
致病机理
;
组织学
;
扫描电子显微镜
;
莲藕丝
|
|
地址
|
1.
淮阴工学院生命科学与食品工程学院, 江苏, 淮安, 223003
2.
江苏省农业科学院植物保护研究所, 江苏, 南京, 210014
3.
南京百特生物工程有限公司, 江苏, 南京, 210019
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
0412-0914 |
|
学科
|
植物保护 |
|
基金
|
财政部和农业农村部:国家现代农业产业技术体系资助
;
江苏省自主创新资金项目
|
|
文献收藏号
|
CSCD:7679076
|
参考文献 共
21
共2页
|
|
1.
Liu Z. Resequencing of 296 cultivated and wild lotus accessions unravels its evolution and breeding history.
The Plant Journal,2020,104(6):1673-1684
|
CSCD被引
8
次
|
|
|
|
|
2.
Man W U. Development status, problems and development ideas of aquatic vegetables industry in China (in Chinese).
长江蔬菜,2019(2):35-41
|
CSCD被引
1
次
|
|
|
|
|
3.
Yuan T Y.
A comparative study on the production and marketing modes of China's main lotus production areas from the perspective of value chain (in Chinese),2021
|
CSCD被引
1
次
|
|
|
|
|
4.
Zhou X J. Study on the biological characteristics of Fusarium oxysporum Schl. f. sp. Nelumbicola (Nis.&Wat.) Booth (in Chinese).
浙江农业学报,2015,27(2):211-214
|
CSCD被引
1
次
|
|
|
|
|
5.
Wei L. Determination method for pathogenicity of artificial inoculated Fusarium pathogen of lotus (in Chinese).
湖南农业科学,2009(7):74-76
|
CSCD被引
1
次
|
|
|
|
|
6.
Wang S F. Occurrence regularity and control measures of lotus root rot (in Chinese).
现代农业科技,2008(8):1
|
CSCD被引
1
次
|
|
|
|
|
7.
Wang B. Chromosome-scale genome assembly of Fusarium oxysporum strain Fo47, a fungal endophyte and biocontrol agent.
Molecular Plant-Microbe Interactions,2020,33(9):1108-1111
|
CSCD被引
2
次
|
|
|
|
|
8.
Li J. Partial pathogenicity chromosomes in Fusarium oxysporum are sufficient to cause disease and can be horizontally transferred.
Environmental microbiology,2020,22(12):4985-5004
|
CSCD被引
1
次
|
|
|
|
|
9.
Yin X. First report of Phytopythium helicoides causing rhizome rot of Asian lotus in China.
Plant Disease,2016,100(2):532
|
CSCD被引
2
次
|
|
|
|
|
10.
Liu X. Research progress on occurrence and control of common diseases and pests of lotus root (in Chinese).
江苏农业科学,2017,45(16):24-28
|
CSCD被引
1
次
|
|
|
|
|
11.
Wang X H.
Identification of the pathogen of lotus root rot and its control (in Chinese),2013
|
CSCD被引
1
次
|
|
|
|
|
12.
Cheng K. Paraffin embedding rice tissue and sectioning.
Bio-101,2018:e1010140
|
CSCD被引
1
次
|
|
|
|
|
13.
Chen W P. A study on ecological anatomy in Nelumbo Nucifera Gaertn (in Chinese).
生态学报,1988,8(3):277-282
|
CSCD被引
1
次
|
|
|
|
|
14.
Duan H.
Study on the structure and properties of lotus fiber (in Chinese),2010
|
CSCD被引
1
次
|
|
|
|
|
15.
Deng S. LAMP assay for distinguishing Fusarium oxysporum and Fusarium commune in lotus (Nelumbo nucifera) rhizomes.
Plant Disease,2022,106(1):231-246
|
CSCD被引
4
次
|
|
|
|
|
16.
Zeng L S. Identification of pathogen causing rhizome rot of lotus (Nelumbo nucifera) based on morphology and multi-gene sequence analysis (in Chinese).
植物病理学报,2017,47(2):162-173
|
CSCD被引
1
次
|
|
|
|
|
17.
Kuang W. Genome and transcriptome sequencing analysis of Fusarium commune provides insights into the pathogenic mechanisms of the lotus rhizome rot.
Microbiology spectrum,2022,10(4):e0017522
|
CSCD被引
4
次
|
|
|
|
|
18.
Cheng P. Research progress of lotus fiber (in Chinese).
成都纺织高等专科学校学报,2016,33(4):120-125
|
CSCD被引
1
次
|
|
|
|
|
19.
Jun X. Biological significance of callose in plants (in Chinese).
生物学通报,2021,56(11):4-6
|
CSCD被引
1
次
|
|
|
|
|
20.
Liu S. Fusaric acid instigates the invasion of banana by Fusarium oxysporum f. sp. cubense TR 4.
New Phytologist,2020,225(2):913-929
|
CSCD被引
11
次
|
|
|
|
|
了解气象卫星更多信息，请访问