中国特有的3个普通小麦亚种抗条锈病评价及其抗性基因分子检测
Evaluation and molecular detection of stripe rust resistance in three subspecies of Chinese endemic wheat
查看参考文献51篇
李菁
1,2
姚方杰
1,2
龙黎
1,2
王昱琦
1,2
叶雪玲
1,2
邓梅
1,2
蒋云峰
1,2
李伟
3
江千涛
1,2
康厚扬
1,2
陈国跃
1,2
*
|
文摘
|
西藏半野生小麦(Triticum aestivum ssp.tibetanum Shao)、云南铁壳麦(T.aestivum ssp.yunnanense King)和新疆稻麦(T.petropavloski Udats et Migusch)是我国特有的3个普通小麦亚种,它们具有特定的地理分布、明显不同于普通小麦的形态学特征,具有抗病、耐逆等优良性状,是可用于现代小麦品种生物和非生物胁迫改良的优异种质资源。本研究利用当前在我国流行频率高、毒性强的条锈菌生理小种和致病类群组成的混合菌对213份中国特有小麦种质(包括117份西藏半野生小麦、78份云南铁壳麦和18份新疆稻麦)进行苗期和成株期抗性表型鉴定,并利用与12个已知条锈病抗性基因(Yr)相关的分子标记进行分子检测。结果表明,共有18份中国特有小麦种质对混合生理小种表现苗期抗性,89份表现成株期抗性。进一步分析发现,成株期抗性种质主要来源于云南铁壳麦。结合系谱分析、抗性表型及条锈病抗病基因分子标记检测结果发现,2份中国特有小麦种质携带Yr18,所有种质均不携带Yr5、Yr9、Yr10、Yr15、Yr17、Yr24/Yr26、Yr30、Yr41、Yr48、Yr65和Yr67等已知抗病基因。未检测到供试基因的抗性种质可能携带其他已知或新的条锈病抗性基因。该研究为进一步有效利用中国特有小麦亚种抗条锈病种质和发掘其抗性基因提供依据。 |
|
其他语种文摘
|
Tibetan semi-wild wheat(Triticum aestivum ssp.tibetanum Shao),Yunnan hulled wheat(T.aestivum ssp.yunnanense King)and Xinjiang rice wheat(T.petropavlovskyi Udacz et Migusch)are unique germplasm resources and have specific geographical distribution in China.Morphological characteristics of them are obviously different from common wheat(T.aestivum L.).Besides,disease resistance and tolerance can be used for improvement of modern wheat variety under biotic and abiotic stress.In this study,213 accessions of Chinese endemic wheat(including 117 Tibetan semi-wild wheat,78 Yunnan hulled wheat and 18 Xinjiang rice wheat)were inoculated with mixed races of wheat stripe rust to evaluate the resistance during seedling and adult stage,respectively.Meanwhile,molecular markers linked to twelve known resistance genes were used to detect the resistance genes.The results showed that 18 accessions were resistant at seedling stage,as well as 89 accessions displayed stable resistance at adult stage.Moreover,these resistant accessions mainly derived from Yunnan hulled wheat.By integrating all information of the pedigree of these accessions,resistance to stripe rust races,and molecular detection,it showed that only 2 accessions carried Yr18 and the 213 accessions were not detected to carry Yr5,Yr9,Yr10,Yr15,Yr17,Yr24/Yr26,Yr30,Yr41,Yr48,Yr65 and Yr67.These results indicated that the resistant accessions may contain other known or new resistance genes.The results will provide the information for further effective utilization of stripe rust resistance germplasms of Chinese endemic wheat subspecies and discovery of their resistance genes. |
|
来源
|
植物病理学报
,2020,50(4):426-441 【核心库】
|
|
DOI
|
10.13926/j.cnki.apps.000330
|
|
关键词
|
西藏半野生小麦
;
新疆稻麦
;
云南铁壳麦
;
条锈病
;
Yr基因
;
分子检测
|
|
地址
|
1.
四川农业大学小麦研究所, 成都, 611130
2.
西南作物基因资源发掘与利用国家重点实验室, 西南作物基因资源发掘与利用国家重点实验室, 成都, 611130
3.
四川农业大学农学院, 成都, 611130
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
0412-0914 |
|
学科
|
植物保护 |
|
基金
|
“十三五”国家重点研发计划
;
四川省国际科技创新合作项目
;
四川省重大科技专项
|
|
文献收藏号
|
CSCD:6819832
|
参考文献 共
51
共3页
|
|
1.
Shao Q Q. Semi-wild wheat from Xizang ( Tibet) ( in Chinese).
遗传学报,1979,6(1):34
|
CSCD被引
1
次
|
|
|
|
|
2.
Dong Y S.
Chinese wheat genetic resources,1999:53-56
|
CSCD被引
1
次
|
|
|
|
|
3.
Dong Y S. Expedition and investigation of Yunnan wheat ( Triticum aestivum ssp. yunnanense King) ( in Chinese).
作物学报,1981,7(3):145-152
|
CSCD被引
1
次
|
|
|
|
|
4.
Yang W Y. Research and utilization of Chinese special common wheat germplasm ( in Chinese).
绵阳农专学报,1993,10(3):15-18
|
CSCD被引
1
次
|
|
|
|
|
5.
Shao Q Q. Semi-wild wheat from Xizang ( Tibet) ( in Chinese).
遗传学报,1980,7(2):149-156
|
CSCD被引
1
次
|
|
|
|
|
6.
Jin S B.
Chinese Wheat Varieties,1964:15-19
|
CSCD被引
1
次
|
|
|
|
|
7.
Chen X R. Genetic studies on existing types and main characters of Yunnan wheat ( in Chinese).
遗传,1980,2(6):17-19
|
CSCD被引
1
次
|
|
|
|
|
8.
Yao J X. Study on a new species of Xinjiang rice wheat ( in Chinese).
遗传,1983,5(1):17-20
|
CSCD被引
1
次
|
|
|
|
|
9.
Chen Q. Cytogenetical studies on interspecific hybrids of Xinjiang wheat ( in Chinese).
作物学报,1985,11(1):23-29
|
CSCD被引
4
次
|
|
|
|
|
10.
Huang L. Analysis of the chromosome constitution of Yunnan wheat ( Triticum aestivum ssp. yunnanense King) with double ditelosomic lines of Triticum aestivum L. cv. Chinese Spring ( in Chinese).
中国农业科学,1989,22(4):13-16
|
CSCD被引
2
次
|
|
|
|
|
11.
Chen P D. Analysis of the genomic constitution of Xizang wheat ( Triticum aestivum ssp. tibetanum Shao) using double ditelosomics of T. aestivum cv. Chinese Spring ( in Chinese).
遗传学报,1991(1):39-43
|
CSCD被引
1
次
|
|
|
|
|
12.
Yang W Y. The study on origin of special kinds of hexaploid wheats from China ( in Chinese).
四川农业大学学报,1992,10(1):12-18
|
CSCD被引
1
次
|
|
|
|
|
13.
Yang R W. Genetic differences and the relationship of gliadin between Triticum polonicum and T. petropavlovskyi ( in Chinese).
麦类作物学报,2000,20(4):1-5
|
CSCD被引
2
次
|
|
|
|
|
14.
Cui Y X. Esterase isozyme of indigenous wheat to China ( in Chinese).
植物学报,1990,32(1):39-44
|
CSCD被引
1
次
|
|
|
|
|
15.
Wei Y M. HMW-glutenin and gliadin variations in Tibetan weedrace,Xinjiang rice wheat and Yunnan hulled wheat.
Genetic Resources and Crop Evolution,2002,49(3):327-330
|
CSCD被引
14
次
|
|
|
|
|
16.
Wei Y M. Genetic diversity of Gli-1,Gli-2 and Glu-1 alleles among Chinese endemic wheats ( in Chinese).
植物学报(英文版),2001,43(8):834-839
|
CSCD被引
1
次
|
|
|
|
|
17.
Ralph R. Chromosomal interchanges and the phylogeny of wheat.
Heredity,1967,22(2):233-247
|
CSCD被引
5
次
|
|
|
|
|
18.
Zhou X H. QTL Mapping for kernel morphology traits of Tibetan semi-wild wheat( in Chinese).
麦类作物学报,2016,36(1):27-35
|
CSCD被引
1
次
|
|
|
|
|
19.
Wang H J. Genetic mapping of loci determining long glumes in the genus Triticum.
Euphytica,2002,123(2):287-293
|
CSCD被引
6
次
|
|
|
|
|
20.
Guo B S. Identification and analysis of salt tolerance in Tibetan wheat ( in Chinese).
植物遗传资源学报,2001,2(2):36-39
|
CSCD被引
1
次
|
|
|
|
|
了解气象卫星更多信息，请访问