慢性强迫游泳应激对大鼠情绪和脑细胞外信号调节激酶磷酸化水平的影响
The effects of chronic forced swimming stress on emotion and brain phospho-extracellular signal-regulated kinases in rats
查看参考文献11篇
|
文摘
|
目的 探讨慢性强迫游泳应激对大鼠情绪和脑细胞外信号调节激酶磷酸化(P-ERK1/2)水平的影响.方法 将30只SD雄性大鼠随机分为游泳应激组、装置对照组和空白对照组,每组10只.游泳应激组每天接受5 min的游泳应激,装置对照组每天接受5 min的新异场景应激,均连续14d,空白对照组不进行任何干预,然后观察大鼠行为(体质量增长量、旷场测验和糖精水溶液偏好测验).采用免疫印迹法测定大鼠海马和前额叶皮质的P-ERK1/2.结果 (1)游泳应激组在应激7 d和应激14 d的体质量增长[分别为(75±22)g和(70±24)g]均低于空白对照组[分别为(101±35)g和(115±47)g],均P＜0.05.(2)装置对照组的粪便排泄量[(1.4±1.9)粒]多于空白对照组[(0.4±1.0)粒]和游泳应激组[(0.1±0.3)粒],均P＜0.05;而游泳应激组的水平活动距离[(2 077±1 245)cm]少于空白对照组[(2 990±1 038)cm]和装置对照组[(3 110±1 462)cm],均P＜0.05.(3)游泳应激组的糖精水溶液摄入量[(11±6)g]和糖精水溶液摄入量占总液体摄入量的比例[(37±16)%]均低于空白对照组[分别为(15±4)g和(47±15)%],均P＜0.05.(4)游泳应激组在海马[(46±95)%]和前额叶皮质[(65±24)%]的P-ERK2水平均低于空白对照组[分别为(76±30)%和(99±42)%],均P＜0.05.结论 慢性强迫游泳应激能诱发大鼠的抑郁情绪,降低P-ERK2在海马和前额叶皮质的水平. |
|
其他语种文摘
|
Objective To investigate the effect of chronic forced swimming stress on emotion and phospho-extracellular signal-regulated kinases of brain in rats. Methods Thirty male Sprague-Dawley rata were randomly divided into three groups with ten in each. The stressed animals were given swimming stress for 5 minutes once daily for 14 days, the apparatus controls were placed in novel environments for 5 minutes once daily for 14 consecutive days, and the controls were free of stress. The behavior was tested after stress, then the rats were decapitated. The tissues of hippocampus and prefrontal cortex were processed for P-ERK1/ 2 detection using Western blotting. Results (1) The body weight gains on the seventh [ (75 ±22) g] and fourteenth stressed days [(70±24)g] were significantly lower in stressed group than that in controls [(101 ±35) gvs. (115 ±47) g, P<0.05]. (2) The defecation in apparatus controls [ (1. 4 ± 1. 9) boli] was more than that in controls [ (0. 4 ± 1. 0) boli] and stressed group [ (0.1 ±0. 3) boli, P <0.05], and the locomotion [ (2 077 ± 1 245) cm] was significantly lower in stressed group than that in controls [ (2 990 ± 1 038) cm] and apparatus controls [ (3 110 ± 1 462) cm, P < 0. 05] . (3) The saccharine solution consumed [(11 ±6) g] and the ratio of the consumed saccharine solution to consumed total liquid [ (37 ± 16) %] in stressed group were significandy lower than that in controls [ (15 ±4) g and (47 ± 15) % respectively, P < 0. 05 ]. (4) The P-ERK2 level in hippocampus [ (46 ± 95) % ] and prefrontal cortex [ ( 65 ± 24).%] was markedly lower in stressed group than that in controls [(76 ±30)% and (99 ±42)% respectively,P < 0. 05] . Conclusions The results indicate that chronic forced swimming stress induce depression, and decrease the P-ERK2 level in hippocampus and prefrontal cortex. |
|
来源
|
中华精神科杂志
,2006,39(3):178-181 【核心库】
|
|
关键词
|
应激
;
情绪
;
海马
;
额叶前皮质
;
有丝分裂素激活蛋白激酶类
;
磷酸化
|
|
地址
|
中国科学院心理研究所脑-行为研究中心, 北京, 100101
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1006-7884 |
|
学科
|
神经病学与精神病学 |
|
基金
|
国家自然科学基金资助项目
;
中国科学院知识创新工程项目
|
|
文献收藏号
|
CSCD:2409994
|
参考文献 共
11
共1页
|
|
1.
Garcia R. Stress.
Rev Neurosci,2002,13:195-208
|
CSCD被引
8
次
|
|
|
|
|
2.
Pearson G. regulation and physiological functions.
Endocr Rev,2001,22:153-183
|
CSCD被引
155
次
|
|
|
|
|
3.
Schafe GE. Activation of ERK/MAP kinase in the amygdala is required for memory consolidation of Pavlovian fear conditioning.
J Neurosci,2000,20:8177-8187
|
CSCD被引
10
次
|
|
|
|
|
4.
Paxinos G. AChE-stained horizontal sections of the rat brain in stereotaxic coordinates.
J Neurosci Methods,1980,3:129-149
|
CSCD被引
35
次
|
|
|
|
|
5.
Bach-Rojecky L. The antidepressant activity of Hypericum perforatum L.
Acta Pharm,2004,54:157-162
|
CSCD被引
4
次
|
|
|
|
|
6.
Wigger A. Periodic maternal deprivation induces gender-dependent alterations in behavioral and neuroendorcrine responses to emotional stress in adult rats.
Physiol Behav,1999,66:293-302
|
CSCD被引
7
次
|
|
|
|
|
7.
Levshina IP. Peculiarities of exploration behavior of socially deprived rats in stress situation.
Zh Vyssh Nerv Deiat Im IP Pavlova,2002,52:602-608
|
CSCD被引
2
次
|
|
|
|
|
8.
Pijlman FT. prepulse inhibition increased after physical.
Eur Neuropsychopharmacology,2003,13:369-380
|
CSCD被引
6
次
|
|
|
|
|
9.
Meller E. Region-specific effects of acute and repeated restraint stress on the phosphorylation of mitogenactivated protein kinases.
Brain Res,2003,979:57-64
|
CSCD被引
16
次
|
|
|
|
|
10.
Murray B. Inhibition of the p44/42MAP kinase pathway protects hippocmapal neurons in a cell-culture model of seizure activity.
Proc Natl Acad Sci USA,1998,95:11975-11980
|
CSCD被引
9
次
|
|
|
|
|
11.
Drevets WC. implications for the cognitive-emotional features of mood disorders.
Curr Opin Neurobiol,2001,11:240-249
|
CSCD被引
32
次
|
|
|
|
|
了解气象卫星更多信息，请访问