苹果茎沟病毒微滴数字RT-PCR检测方法的建立和应用
Establishment and application of droplet digital RT-PCR detection method for apple stem grooving virus
查看参考文献27篇
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文摘
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我国是第一大苹果生产国。苹果茎沟病毒(apple stem grooving virus,ASGV)在我国各苹果产区分布广泛,侵染果树导致树势变弱,生长量减少,果实品质下降,严重威胁我国苹果产业的可持续发展。病毒检测是果树病毒病防控的重要前提。本研究根据苹果茎沟病毒外壳蛋白基因保守序列,设计了引物和探针,建立了微滴数字PCR(droplet digital PCR, ddPCR)检测方法。所建立的检测方法能特异性检出苹果茎沟病毒,检测重复性好,灵敏度比qRT-PCR法高10倍,可适用于田间样品及组培苗检测。该方法的建立为苹果茎沟病毒的检测提供了重要的技术手段。 |
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其他语种文摘
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China is the largest apple producer in the world. Apple stem grooving virus (ASGV), which impairs the growth and yield of apple trees, is widely distributed in apple producing areas of China. Virus detection is crucial for the prevention and control of apple viral diseases. In this research, we designed primers and probes according to the conserved regions of ASGV coat protein gene. A droplet digital PCR (ddPCR) for ASGV detection was established and assessed. The results showed that the ddPCR could detect ASGV specifically with high repeatability. The sensitivity of ddPCR is 10 times higher than that of qPCR. The ddPCR established in this research could be applied for virus detection in field samples of orchard and virus-free seedlings. |
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来源
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植物病理学报
,2024,54(2):436-442 【核心库】
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DOI
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10.13926/j.cnki.apps.001330
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关键词
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苹果茎沟病毒
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微滴数字PCR
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检测
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地址
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西北农林科技大学植物保护学院, 杨凌, 712100
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0412-0914 |
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学科
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植物保护 |
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基金
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陕西省重大研发专项
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陕西省重点研发计划
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文献收藏号
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CSCD:7863507
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参考文献 共
27
共2页
|
|
1.
Wang S T. Occurrence and research progress of important apple diseases in China in recent years (in Chinese).
植物保护,2018,44(5):13-25,50
|
CSCD被引
1
次
|
|
|
|
|
2.
Hao L. Occurrence and detection of virus diseases in some major apple producing areas in northern China (in Chinese).
植物保护,2015,41(2):158-161
|
CSCD被引
3
次
|
|
|
|
|
3.
Dong Y H. Strengthen standardized sampling and virus detection of fruit trees to reduce the risk of hidden and dangerous viruses to China' s apple industry (in Chinese).
植物保护,2020,46(2):164-168
|
CSCD被引
2
次
|
|
|
|
|
4.
Hu G J. Major apple viruses in China and their research progress (in Chinese).
中国果树,2017,185(3):71-74,82
|
CSCD被引
2
次
|
|
|
|
|
5.
Tan J Q. Preparation and application of antibody against apple stem grooving virus coat protein (in Chinese).
西北农业学报,2020,29(5):787-792
|
CSCD被引
1
次
|
|
|
|
|
6.
Guo L X. Research progress of apple stem grooving virus (in Chinese).
果树学报,2008,25(5):714-720
|
CSCD被引
1
次
|
|
|
|
|
7.
Guo L X. Research progress of apple stem grooving virus (in Chinese).
果树学报,2008,25(5):6-8
|
CSCD被引
1
次
|
|
|
|
|
8.
Magome H. Single-strand conformation polymorphism analysis of apple stem grooving capillovirus sequence variants.
Phytopathology,1999,89(2):136-140
|
CSCD被引
4
次
|
|
|
|
|
9.
Li Z N. Sequence analysis of the whole genome of an apple stem grooving virus isolated from Jilin shaguo (in Chinese).
园艺学报,2018,45(4):641-649
|
CSCD被引
1
次
|
|
|
|
|
10.
Liu X. Research progress of digital PCR in precise detection of functional nucleic acids (in Chinese).
生物技术通报,2018,34(9):149-162
|
CSCD被引
1
次
|
|
|
|
|
11.
Zou Y. Nucleic acid purification from plants, animals and microbes in under 30 seconds.
PLoS Biology,2017,15(11):e2003916
|
CSCD被引
23
次
|
|
|
|
|
12.
Salipante S J. Digital PCR-an emerging technology with broad applications in microbiology.
Clinical Chemistry,2020,66(1):117-123
|
CSCD被引
17
次
|
|
|
|
|
13.
Sreejith K R. Digital polymerase chain reaction technology-recent advances and future perspectives.
Lab on a Chip,2018,18(24):3717-3732
|
CSCD被引
2
次
|
|
|
|
|
14.
Fu W. A highly sensitive and specific method for the screening detection of genetically modified organisms based on digital PCR without pretreatment.
Scientific Reports,2015,5(1):1-10
|
CSCD被引
20
次
|
|
|
|
|
15.
Zhu P Y. Ultrasensitive and absolute quantitative detection of Cu~(2+) based on DNAzyme and digital PCR in water and drink samples.
Food Chemistry,2017,221:1770-1777
|
CSCD被引
5
次
|
|
|
|
|
16.
Zhang Y J. Microdrop digital PCR (ddPCR) for detection of potato virus S (PVS) (in Chinese).
农业生物技术学报,2017,25(10):1721-1728
|
CSCD被引
1
次
|
|
|
|
|
17.
Wang J. Establishment and application of reverse transcription digital PCR detection method for Newcastle disease virus type VI (in Chinese).
中国动物检疫,2022,39(9):115-120
|
CSCD被引
1
次
|
|
|
|
|
18.
Pinheiro L B. Evaluation of a droplet digital polymerase chain reaction format for DNA copy number quantification.
Analytical Chemistry,2012,84(2):1003-1011
|
CSCD被引
70
次
|
|
|
|
|
19.
Hindson B J. High-throughput droplet digital PCR system for absolute quantitation of DNA copy number.
Analytical Chemistry,2011,83(22):8604-8610
|
CSCD被引
116
次
|
|
|
|
|
20.
Chen Y N. Establishment of a quantitative detection method for pseudorabies virus micro drop digital PCR (in Chinese).
畜牧兽医学报,2017,48(9):1705-1710
|
CSCD被引
2
次
|
|
|
|
|
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