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甜樱间座壳菌( Diaporthe eres)引起苹果根颈腐烂病研究简报
Brief report of apple diaporthe neck and root rot caused by Diaporthe eres

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莫艳芳 1,2   普特 2   罗强 1,2   施竹凤 2   杨童雨 1,2   矣小鹏 1,2   申云鑫 1,2   王楠 1,2   廖永琴 1,2   何永宏 1   陈齐斌 1 *   杨佩文 2 *  
文摘 随着云南高原特色农业各项工作的深入实施,苹果产业成为区域农业产业的重要支撑,是广大山区半山区农民脱贫致富可靠的经济创收途径。另一方面,根腐病是近年来在苹果主产区发生最广泛、危害最严重的病害之一,发病率一般在10%左右,严重的达50%以上。根腐病主要危害作物根部,病程长,初期很难发现,一旦地上表现出发病症状,很难达到理想的防治效果,常常造成严重的产量和经济损失,是制约云南苹果产业发展最重要的限制性因素之一。
其他语种文摘 In recent years,apple diaporthe neck and root rot has become one of the most important limiting factors for the development of apple production in Yunnan Province. In this study,the typical disease samples were collected and the fungal isolate M2g7-1 was obtained by tissue separation approach. The purified culture M2g7-1 was preliminary determined belonging to Diaporthe spp. based on the morphological features of colony,pycnidia, cirrus,two forms of conidia. The pathogenicity of M2g7-1 was further validated on apple young branches to fulfill the Koch's law. The taxonomic of pathogen M2g7-1 has been further determined with combining molecular phylogenetics. The phylogenetic tree was created with the data set of sequences from the internal transcribed spacer ( ITS) of ribosomal DNA,the histone H3 ( HIS) gene,the translation elongation factor 1-alpha ( TEF) gene,and the beta-tubulin ( TUB) gene. Based on morphological and molecular biology analysis,the pathogen M2g7-1 was finally identified as Diaporthe eres. This is the first report of D. eres as the pathogen of apple diaporthe neck and root rot. The research results provide a theoretical basis for the epidemic research and comprehensive control of this disease.
来源 植物病理学报 ,2024,54(5):1036-1041 【核心库】
DOI 10.13926/j.cnki.apps.001041
关键词 甜樱间座壳菌 ; 苹果 ; 根颈腐烂病
地址

1. 云南农业大学植物保护学院, 云南, 650201  

2. 云南省农业科学院农业环境与资源研究所, 云南, 652025

语种 中文
文献类型 研究性论文
ISSN 0412-0914
学科 植物保护
基金 云南省科技计划重大科技专项 ;  国家自然科学基金
文献收藏号 CSCD:7863575

参考文献 共 14 共1页

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引证文献 1

1 罗强 苹果白纹羽根腐病菌褐座坚壳菌生物学特性及寄主范围测定 西南农业学报,2025,38(2):334-340
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