文摘
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目的:建立藏药二十五味鬼臼丸的定性定量方法。方法:采用TLC法对二十五味鬼臼丸中的沉香、诃子进行鉴别;采用RP—HPLC法,Symmetry C_(18)色谱柱(150mm×4.6mm,50μm),以甲醇-水(60:40)为流动相,流速1.0mL·min~(-1),检测波长为343nm,柱温30℃,进样量10μL,对二十五味鬼臼丸中的胡椒碱进行含量测定。结果:薄层斑点清晰,专属性强;胡椒碱浓度在2.24—11.20μg·mL~(-1)范围内线性关系良好(r=0.9991),平均回收率(n=6)为97.4%(RSD为1.7%)。结论:该方法快速、简单、准确,重复性好,可有效控制二十五味鬼臼丸的质量。 |
其他语种文摘
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Objective:To establish the qualitative and quantitative methods of Ershiwuwei Guijiu pills. Method: Lignum Aquilariae Resinatum and Fructus Chebulae were identified by TLC, and piperine content in Fructus Piperis was determined by HPLC. Symmetry C_(18) column( 150 mm× 4. 6 mm,5 μm) was used. The mobile phase was methanol - water(60: 40) at a flow rate of 1.0 mL · min~(-1), the detection wavelength was set at 343 nm, column temperature was 30 ℃ ,and the injection volume was 10 μL. Results:The TLC identification was highly specific and spots were clear. Piperine showed a good linear relationship in the range of 2. 24 - 11.2 μg · mL~(-1) (r =0. 9991 ) ;The average recovery(n =6) was 97.4%, RSD = 1.7%. Conclusion:The method is simple, reliable and has a good repeatability, and can be used for the quality control of Ershiwuwei Guijiu pills. |
来源
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药物分析杂志
,2008,28(1):108-110 【核心库】
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关键词
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二十五味鬼臼丸
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胡椒碱
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定性定量
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薄层色谱
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反相高效液相色谱
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地址
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中国科学院西北高原生物研究所, 青海, 西宁, 810001
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语种
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中文 |
文献类型
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研究性论文 |
ISSN
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0254-1793 |
学科
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药学 |
文献收藏号
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CSCD:3224483
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