中压制备色谱法分离制备油橄榄叶中多酚类化合物
Separation and preparation of polyphenols from leaves of Olea europaea by preparative medium pressure liquid chromatography

夏雅俊刘永峰裴栋邸多隆 ^*

文摘	目的研究油橄榄 Olea europare叶抗糖尿病有效部位的药效物质基础。方法利用反相中压制备液相色谱技术进行分离纯化,并应用现代波谱技术鉴定化合物的结构。结果从油橄榄叶抗糖尿病活性部位中分离并鉴定得到的5个化合物均为多酚类化合物,分别为脱咖啡酰基毛蕊糖苷(1)、木犀草素-7-O-β-D-葡萄糖苷(2)、6″-O-β-D-吡喃葡萄糖-橄榄苦苷(3)、裂环马钱子苷(4)、橄榄苦苷(5)。结论化合物1为首次从该植物中分离得到。5种化合物经HPLC检测,按照归一化法计算其质量分数均大于90%,为以油橄榄叶为原料的新药开发提供技术支撑。
其他语种文摘	Objective To study the material basis of the anti-diabetic effective fraction in the leaves of Olea europaea. Methods Preparative medium pressure liquid chromatography (PMPLC) was used for the separation and purification of the compounds. Their structures were elucidated by spectral analyses. Results The structures of the compounds were identified by various spectroscopic methods combined with the data from some published literatures. There were five compounds which were extracted from the anti-diabetic effective fraction in the leaves of O. europaea and identified as decaffeoylverbascoside (1), luteolin-7-O-β-D-glucoside(2), 6″-O-β-D-glucopyranosyl oleuropein (3), oleuroside (4), and oleuropein (5). Additionally, decaffeoylverbascoside is isolated from this plant for the first time and the purities of the five compounds were all above 90% by HPLC detection. Conclusion This research provides technological support for the development of new drug based on the leaves of O. europaea.
来源	中草药 ,2014,45(12):1689-1692 【核心库】
DOI	10.7501/j.issn.0253-2670.2014.12.006
关键词	中压制备色谱 ; 油橄榄 ; 抗糖尿病 ; 多酚 ; 脱咖啡酰基毛蕊糖苷
地址	中国科学院兰州化学物理研究所, 中国科学院西北特色植物资源化学重点实验室;;甘肃省天然药物重点实验室, 甘肃, 兰州, 730000
语种	中文
文献类型	研究性论文
ISSN	0253-2670
学科	中国医学
基金	国家自然科学基金资助项目 ; 中国科学院“百人计划”项目
文献收藏号	CSCD:5198678