帮助 关于我们

返回检索结果

The role of TRPC6 in HGF-induced cell proliferation of humanprostate cancer DU145 and PC3 cells

查看参考文献29篇

Wang Yong 1   Yue Dan 2   Li Kai 3   Liu Yili 1   Ren Changshan 4   Wang Ping 1  
文摘 Hepatocyte growth factor (HGF) is a glycoprotein that induces prostate cancer cell proliferation, migration and invasion. The activation of transient receptor potential canonical 6 (TRPC6) channels is considered important inpromoting prostate cancer cell proliferation. In this study, we assessed the role of endogenous TRPC6 channels inthe HGF-induced cell proliferation of prostate cancer. Reverse transcription-PCR and Western blotting were usedto investigate TRPC6 expression. Electrophysiological techniques (whole-cell patch clamp configuration) and Ca~(2+) imaging analysis were used to investigate the channel activity in cells. The effects of TRPC6 channels on cell cycleprogression, cell apoptosis and cell growth were also examined. TRPC6 and c-MET were expressed in DU145 andPC3 cells. In addition, functional TRPC6 channels were present in DU145 and PC3 cells, and TRPC6 knockdownsuppressed TRPC-like currents evoked by oleoyl-2-acetyl-sn-glycerol (OAG). Inhibition of TRPC6 channels in DU145 and PC3 cells abolished OAG- and HGF-induced Ca~(2+) entry. Furthermore, inhibition of TRPC6 channels arrested DU145 and PC3 cells at the G_2/M phase and suppressed HGF-induced cell proliferation. Collectively, ourresults indicate that TRPC6 has an important role in HGF-induced DU145 and PC3 cell proliferation
来源 Asian Journal of Andrology ,2010,12(6):841-852 【核心库】
DOI 10.1038/aja.2010.85
关键词 hepatocyte growth factor ; human prostate cancer ; proliferation ; TRPC6
地址

1. Department of Urology, Fourth Affiliated Hospital, China Medical University, Shenyang, 110032  

2. College of Medical Laboratory Science, Tianjin Medical University, Tianjin, 300070  

3. Department of Surgical Oncology, First Affiliated Hospital, China Medical University, Shenyang, 110001  

4. Cancer Research Institute, First Affiliated Hospital, China Medical University, Shenyang, 110001

语种 英文
文献类型 研究性论文
ISSN 1008-682X
学科 特种医学
文献收藏号 CSCD:4073832

参考文献 共 29 共2页

1.  Feldman BJ. The development of androgen-independent prostate cancer. Nat Rev Cancer,2001,1:34-45 CSCD被引 57 次    
2.  Pardo LA. Eagl: an emerging oncological target. Cancer Res,2008,68:1611-1613 CSCD被引 5 次    
3.  Roderick HL. Ca~(2+) signalling checkpoints in cancer: remodelling Ca~(2+) for cancer cell proliferation and survival. Nat Rev Cancer,2008,8:361-375 CSCD被引 23 次    
4.  Kahl CR. Regulation of cell cycle progression by calcium/calmodulin-dependent pathways. Endocr Rev,2003,24:719-736 CSCD被引 24 次    
5.  Vanoverberghe K. Ca~(2+) homeostasis and apoptotic resistance of neuroendocrine-differentiated prostate cancer cells. Cell Death Differ,2004,11:321-330 CSCD被引 7 次    
6.  Hardie RC. The trp gene is essential for a lightactivated Ca~(2+) channel in Drosophila photoreceptors. Neuron,1992,8:643-651 CSCD被引 15 次    
7.  Ramsey IS. An introduction to TRP channels. Annu Rev Physiol,2006,68:619-647 CSCD被引 52 次    
8.  Guilbert A. Expression of TRPC6 channels in human epithelial breast cancer cells. BMC Cancer,2008,8:125 CSCD被引 5 次    
9.  El Boustany C. Capacitative calcium entry and transient receptor potential canonical 6 expression control human hepatoma cell proliferation. Hepatology,2008,47:2068-2077 CSCD被引 14 次    
10.  Cai R. Blockade of TRPC6 channels induced G2/M phase arrest and suppressed growth in human gastric cancer cells. Int J Cancer,2009,125:2281-2287 CSCD被引 6 次    
11.  Thebault S. Alphal- adrenergic receptors activate Ca~(2+)-permeable cationic channels in prostate cancer epithelial cells. J Clin Invest,2003,111:1691-1701 CSCD被引 3 次    
12.  Thebault S. Differential role of transient receptor potential channels in Ca~(2+) entry and proliferation of prostate cancer epithelial cells. Cancer Res,2006,66:2038-2047 CSCD被引 11 次    
13.  Hashem M. Hepatocyte growth factor as a tumor marker in the serum of patients with prostate cancer. J Egypt Natl Canc Inst,2005,17:114-120 CSCD被引 3 次    
14.  Yasuda K. Serum active hepatocyte growth factor (AHGF) in benign prostatic disease and prostate cancer. Prostate,2009,69:346-351 CSCD被引 3 次    
15.  Kawanishi T. Hepatocyte growth factor-induced calcium waves in hepatocytes as revealed with rapid scanning confocal microscopy. Cell Calcium,1995,18:495-504 CSCD被引 1 次    
16.  Rampino T. KCNA1 and TRPC6 ion channels and NHE1 exchanger operate the biological outcome of HGF/scatter factor in renal tubular cells. Growth Factors,2007,25:382-391 CSCD被引 3 次    
17.  Yue D. Expression of TRPC6 in benign and malignant human prostate tissues. Asian JAndrol,2009,11:541-547 CSCD被引 3 次    
18.  Yue D. Effects of transferred NK4 gene on proliferation, migration,invasion and apoptosis of human prostate cancer DU145 cells. Asian JAndrol,2010,12:381-389 CSCD被引 6 次    
19.  Hamdollah Zadeh MA. VEGF-mediated elevated intracellular calcium and angiogenesis in human microvascular endothelial cells in vitro are inhibited by dominant negative TRPC6. Microcirculation,2008,15:605-614 CSCD被引 1 次    
20.  Hofmann T. Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol. Nature,1999,397:259-263 CSCD被引 14 次    
引证文献 1 篇

1 王朕华 TRPC6在子宫内膜癌组织中的表达及对细胞增殖作用的研究 中国实用妇科与产科杂志,2019,35(5):569-573
CSCD被引 1 次

显示所有1篇文献

论文科学数据集
PlumX Metrics
相关文献

 作者相关
 关键词相关
 参考文献相关

版权所有 ©2008 中国科学院文献情报中心 制作维护：中国科学院文献情报中心
地址：北京中关村北四环西路33号 邮政编码：100190 联系电话：(010)82627496 E-mail:cscd@mail.las.ac.cn 京ICP备05002861号-4 | 京公网安备11010802043238号