文摘
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利用反相高效液相色谱法,采用Supelcosil LC-18色谱柱(250 mm * 4.6 mm i.d.,5μm ),以甲醇-0.05 mol/L KH_2PO_4缓冲液(体积比为20:80)为流动相,流速0.8 mL/min,在254 nm波长处检测,对生活在高原(海拔2.3 km)的习服大鼠肝组织中脱氧核糖核酸(DNA)的碱基含量进行了检测,发现各碱基在DNA中所占的比例是相对稳定的:腺嘌呤(A)28.8%、鸟嘌呤(G)23.3%、胞嘧啶(C)17.4%、胸腺嘧啶(T)25.3%,并利用内标法对DNA甲基化水平进行了测定。 |
其他语种文摘
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The base contents of liver deoxyribonucleic acid (DNA) of rats living at an altitude of 2.3 km were determined by reversed-phase high performance liquid chromatography. At first, 0.05 mol/L KH_2PO_4 (pH 4.0) was used to dissolve the DNA acid hydrolysis products with 8-bromoguanosine (Br~8G) as an internal standard. Then the DNA hydrolysis products with Br~8G were chromatographed on a Supelcosil LC-18 column with UV detection at 254 nm and eluted by the mobile phase of MeOH 0.05 mol/L KH_2PO_4 (pH 4.0) (20:80, V/V) at the flow rate of 0.8 mL/min. Under these conditions, several bases were separated effectively. From the results, the relatively constant proportions of the bases in DNA were found. The contents were 17.4% of cytosine (C), 28.8% of adenine (A), 23.3% of guanine (G) and 25.3% of thymine (T). RSDs of the deteremination of these bases were 1.7%, 1.5%, 1.3% and 2.1%, respectively. At the same time the methylation level of liver DNA of the rats determined by the internal standard method was 6.2%. |
来源
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色谱
,2002,20(4):348-349 【核心库】
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关键词
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反相高效液相色谱法
;
脱氧核糖核酸
;
碱基
;
甲基化水平
;
肝组织
;
大鼠
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地址
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中国科学院西北高原生物研究所, 青海, 西宁, 810001
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语种
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中文 |
文献类型
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研究性论文 |
ISSN
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1000-8713 |
学科
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化学 |
文献收藏号
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CSCD:1043520
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