2012年部分麦区小麦白粉菌群体对三唑酮敏感性及其与毒性的关系
Triadimefon sensitivity and its correlation with the virulence population of Blumeria graminis f. sp. tritici in some wheat growing areas in 2012
查看参考文献19篇
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文摘
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抗病品种和化学防治是控制植物病害最有效的两种措施,病原菌在长期选择压下可改变其群体结构,以逐步适应品种抗性和杀菌剂压力。本研究采用拌种离体叶段法对2012年采自我国9个省(市)的129个小麦白粉病菌Blumeria grami-nis f. sp. tritici单孢堆分离物进行了三唑酮敏感性检测,同时采用31个已知抗病基因品种(系)对其进行苗期毒性测定,并对二者之间的相关性进行分析结果表明:129个供试菌株对三唑酮的EC_(50)为109.97 mg/L,平均抗药性水平达到52.62倍,变异系数为107.2。所有供试菌株中,99.22%的菌株产生了抗药性,其中高抗菌株占58.91%,中抗菌株占37.98%。北京菌株的抗药性水平明显高于其他8个省市。利用Popgenl.32软件对9个省(市)小麦白粉病菌群体的毒性多样性分析结果表明,四川群体毒性基因多样性水平最高,Nei基因多样性H值为0.2241,浙江群体最低,H值为0.096 8。小麦白粉菌群体对三唑酮的敏感性和毒性的相关性分析表明,EC_(50)或EC_(50)变异系数与毒性多样性之间均不存在显著的相关性,但EC_(50)与毒性基因数目之间的关系符合对数方程。此结果可为杀菌剂和抗性品种的合理利用提供依据。 |
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其他语种文摘
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Host resistance and fungicides are two of the main approaches used to control plant diseases in conventional agriculture. The widespread use of host resistance and fungicides selects for pathogen individuals or populations that can overcome the host defense systems or that are resistant to the applied fungicides. One hundred and twenty nine isolates of Blumeria graminis f. sp. tritici were collected from 9 provinces/cities in 2012, and inoculated onto 31 differential wheat lines of powdery mildew to test the virulence. The sensitivity of the isolates to triadimefon was tested by seed treatment and detached leaf segment methods. The correlation between virulence diversity and triadimefon-resistance was carried out by SAS software. The results showed that median EC_(50) of isolates to triadimefon was 109.97 mg/L, the coeffective of EC_(50) was 107.2,the mean resistance factor (RF) was 52.62.The resistant frequency of all isolates to triadimefon was up to 99.22%. The mean resistant factors of B. graminis f. sp. tritici isolates from Beijing was higher compared to the other eight provinces. Virulence diversity of B. graminis f. sp. tritici isolates from Sichuan Province was highest with the index of virulence gene diversity 0.224 1. The virulence gene diversity of isolates from Zhejiang was lowest with the index 0.0968. The association between triadimefon-sensitivity and virulence diversity of B. graminis f. sp. tritici isolates showed that there was no significant correlation among EC_(50), variation coefficient of EC_(50) and virulence diversity. However, there is a logarithmic (r=0.240 4,P=0.009 6) relationship between EC_(50) and numbers of virulence genes of 129 B. graminis f. sp. tritici isolates. These results may provide a reference for reasonable utilization of wheat resistance varieties, as well as the use of triazole fungicides. |
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来源
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植物病理学报
,2015,45(2):181-187 【核心库】
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DOI
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10.13926/j.cnki.apps.2015.02.009
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关键词
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小麦白粉菌
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三唑酮
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抗药性
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毒性
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相关性分析
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地址
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中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京, 100193
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0412-0914 |
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学科
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植物保护 |
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基金
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国家973计划
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国家公益性行业（农业）科研专项
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科技支疆项目
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国家自然科学基金
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文献收藏号
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CSCD:5388104
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参考文献 共
19
共1页
|
|
1.
McIntosh R A. Breeding wheat for resistance to biotic stresses.
Wheat:Prospects for Global Improvement,1997:71-86
|
CSCD被引
1
次
|
|
|
|
|
2.
Persaud R R. Virulence genes and virulence gene frequencies of Blumeria graminis f. sp. tritici in Ohio.
Plant disease,1995,79(5):494-499
|
CSCD被引
4
次
|
|
|
|
|
3.
Wolfe M S. Trying to understand and control powdery mildew.
Plant Pathology,1984,33(4):451-466
|
CSCD被引
3
次
|
|
|
|
|
4.
Collina P. Resistance and cross resistance of Erysiphe graminis to triazole and morpholine fungicides.
The First International Powdery Mildew Conference,1999:41
|
CSCD被引
1
次
|
|
|
|
|
5.
Zhou Y L. Development of chemical control for powdery on plants (in Chinese).
农药学学报,2001,3(2):12-18
|
CSCD被引
6
次
|
|
|
|
|
6.
Xia Y. Monitoring of Blumeria graminis f. sp. tritici isolates to triadimefon in 2002 and establishment of pathogen base-line sensitivity to kresoxim-methyl (in Chinese).
植物病理学报,2005,35(6):74-78
|
CSCD被引
5
次
|
|
|
|
|
7.
Cao X R. Monitoring of Blumeria graminis f. sp. tritici isolates to triadimefon in 2007 (in Chinese).
植物保护,2008,34(6):74-77
|
CSCD被引
5
次
|
|
|
|
|
8.
Zhang Y.
Monitoring of resistance of Blumeria graminis f. sp. tritici isolates to triadimefon and the RAPD markers of the isolates of the different fungicide resistance (in Chinese),2001
|
CSCD被引
2
次
|
|
|
|
|
9.
Yang P. Sensitivity of Blumeria graminis f. sp. tritici isolates to triadimefon and quinoxyfen in China in 2008 and its cross-resist-ance between triadimefon and quinoxyfen (in Chinese).
植物病理学报,2010,40(4):404-410
|
CSCD被引
3
次
|
|
|
|
|
10.
Wang L. Sensitivity of Blumeria graminis f. sp. tritici isolates to triadimefon and azoxystrobin in 2009 in China (in Chinese).
植物病理学报,2011,40(6):654-658
|
CSCD被引
2
次
|
|
|
|
|
11.
Si Q M. Identification of physiologic race of Erysiphe graminis f. sp. tritici.
中国农业科学,1987,20(5):64-70
|
CSCD被引
6
次
|
|
|
|
|
12.
Ma Z Q. Methods for monitoring the sensitivity of wheat powdery mildew to triadimenfon.
华北农学报,1996,11(1):93-96
|
CSCD被引
1
次
|
|
|
|
|
13.
Yang J S. The resistance monitoring Blumeria graminis in the Northeast of China.
Journal of Shenyang Agricultural University,1998,29(3):201-204
|
CSCD被引
1
次
|
|
|
|
|
14.
Yu D Z.
Wheat powdery mildew in central China:pathogen population structure and host resistance. PhD thesis,2000
|
CSCD被引
1
次
|
|
|
|
|
15.
Yang L. Sensitivity of Blumeria gra minis f. sp. tritici isolates to triadimefon and fenpropidin in China in 2011 (in Chinese).
植物病理学报,2013,43(4):430-434
|
CSCD被引
1
次
|
|
|
|
|
16.
Yang L. Association between virulence and triazole tolerance in the phytopathogenic fungus Mycosphaerella graminicola.
PLoS ONE,2013,8(3):e59568
|
CSCD被引
1
次
|
|
|
|
|
17.
Limpert E. Fungicide resistance in populations of plant pathogens:determination, correlations with virulence, and recent evolution of resistance to triadimenol in the barley mildew pathogen in Europe.
Integrated control of cereal mildews:Virulence patterns and their change. Proceedings,1991:177-185
|
CSCD被引
1
次
|
|
|
|
|
18.
Chen P R. An oxidation-sensing mechanism is used by the global regulator MgrA in Staphylococcus aureus.
Nature Chem Biol,2006,2:591-595
|
CSCD被引
5
次
|
|
|
|
|
19.
Gupta A. Functional analysis of a novel ABC transporter ABC4 from Magnaporthe grisea.
FEMS Microbiol Lett,2008,278:22-28
|
CSCD被引
5
次
|
|
|
|
|
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