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层状材料水滑石固定青霉素酰化酶的研究
Numerical simulation of abrasive erosion in the liquid-solid two-phase rotary flow
查看参考文献7篇
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文摘
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以层状材料水滑石（LDH）为载体，采用不同酸性氨基酸插层，通过直接吸附和戊二醛交联的方法进行青霉素酰化酶（PGA）的固定。考察了各个因素对固定化酶酶活性的影响，优化了反应条件。制得的固定化酶的表观酶活性达9.67*10~(-6) mol/s，酶活性回收率56.6%。对固定化酶的操作稳定性作了初步探讨。 |
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其他语种文摘
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This issue described a method of immobilizing Penicillin G Acylase (PGA), using a layer material-Layered Double Hydroxide (LDH) intercalated by different sour amino acids as a carrier, with adsorbing PGA directly or glutaraldehyde crosslinking. Several factors that affected the activity of immobilized PGA were studied. The apparent activity and yield of immobilized PGA were 9.67*10~(-6) mol/s, and 56.6%, respectively. The operation stability of immobilized PGA was also discussed. |
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来源
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北京化工大学学报. 自然科学版
,2002,29(3):9-11,16 【扩展库】
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关键词
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青霉素酰化酶
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水滑石
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固定化酶
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谷氨酸插层
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地址
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1.
北京化工大学化学工程学院, 北京, 100029
2.
北京化工大学, 可控化学反应科学与技术基础教育部重点实验室, 北京, 100029
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1671-4628 |
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学科
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化学工业 |
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基金
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国家自然科学基金
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北京市自然科学基金
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文献收藏号
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CSCD:957347
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参考文献 共
7
共1页
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1.
Fonseca L P. Immobilization of an industrial penicillin acylase preparation on a silica carrier.
J Chem Tech Biotechnol,1993,58:27-37
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被引
4
次
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|
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2.
Jose Luis Barbero. Complete nucleotide sequence of the penicillin acylase gene from Klyyvera citrophila.
Gene,1986,49:69-80
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被引
1
次
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|
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3.
胡智峰.
层状材料固定化青霉素酰化酶的研究,2000
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被引
2
次
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|
|
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4.
Walter T. Catalytic reaction by thermally activated, synthetic, anionic minerals.
J Catalysis,1985,94:547-557
|
被引
1
次
|
|
|
|
|
5.
Cavani F. Hydrotalcite-type anionic clays preparation, properties and applications.
Catal Today,1991(11):173-301
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被引
455
次
|
|
|
|
|
6.
Nicola T Whilton. Bioinorganic clays: Synthesis and characterization of amino - and polyamino acid intercalated layered double hydroxides.
J Mater Chem,1997,7(8):1623-1629
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被引
13
次
|
|
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7.
Pramod B Mahajan. Penicillin acylase:An update.
Appl Biochemistry and Biotechnology,1984(9):537-554
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被引
2
次
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