甘肃典型高寒草原退化植物瑞香狼毒对根际土壤微生物群落的影响研究
Effects of degraded plant Stellera chamaejasme L.on the rhizosphere soil microbial communities in typical alpine grassland, Gansu Province
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文摘
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【目的】瑞香狼毒(Stellera chamaejasme L.)为瑞香科狼毒属(Stellera)多年生草本植物,是我国草地退化的标志性植物之一。本研究旨在探究甘肃高寒草原主要毒草瑞香狼毒根际微生物群落结构及其与土壤环境因子和酶活性之间关系,为治理因瑞香狼毒入侵引起的草地退化提供理论依据。【方法】采用Illumina MiSeq高通量测序技术对甘肃不同地区高寒草原的瑞香狼毒根际土壤微生物组成及多样性进行分析,并进一步分析土壤理化性质和酶活性与微生物群落的相关性。【结果】不同地区瑞香狼毒根际土壤pH随海拔升高呈现上升趋势,土壤中大量和微量营养元素含量以及土壤酶活性的变化各异。在门水平上,子囊菌门(Ascomycota)、接合菌门(Zygomycota)和担子菌门(Basidiomycota)在根际土壤真菌中占优势地位;放线菌门(Actinobacteria)、变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)在根际土壤细菌中属于优势类群。海拔2964 m样地的真菌和细菌OTU (operational taxonomic unit)数量和Shannon多样性指数均高于其他4个采样点(海拔2373、2608、2733、3280 m)。RDA (redundancy analysis)分析结果显示,瑞香狼毒根际土壤微生物不同,受土壤环境因子的影响不同;相关系数表明,土壤真菌多样性与土壤钾、磷、铁、钙、钼、海拔、土壤水分、过氧化物酶呈正相关,与土壤温度、多酚氧化酶、脱氢酶、蔗糖酶、碱性磷酸酶呈负相关;土壤细菌与土壤pH、钾、磷、镁、钙、土壤水分呈负相关,与多酚氧化酶、过氧化物酶、脲酶、脱氢酶、蔗糖酶、酸性磷酸酶、碱性磷酸酶呈正相关。【结论】甘肃省不同地区高寒草原瑞香狼毒根际微生物群落组成和多样性差异明显,土壤理化性质与土壤真菌具有较强的正相关关系,而土壤酶则更多地影响着土壤细菌群落的组成和多样性。 |
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其他语种文摘
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[Objective] Stellera chamaejasme L., a perennial herb of Stellera,is one of symbolic plants of grassland degradation in China.The purpose of this study was to explore the microbial community structure in the rhizosphere of Stellera chamaejasme L.and its relationship with soil environmental factors and enzyme activity in Gansu alpine steppe, so as to provide a theoretical basis for controlling grassland degradation caused by Stellera chamaejasme L.invasion.[Methods] The Illumina MiSeq high-throughput sequencing technology was used to analyze the composition and diversity of rhizosphere soil microbes of Stellera chamaejasme L.in various regions of Gansu, and further analyze the relationship between soil physical and chemical properties, enzyme activities and microbial communities.[Results] The results indicated that the pH value of the rhizosphere soil of Stellera chamaejasme L.in different areas increased with altitude, showing an upward trend.The content of macro and micronutrient elements in the soil, and the changes of soil enzyme activities were different.At the phylum level, Ascomycota, Zygomycota and Basidiomycota are dominant in rhizosphere soil fungi.Actinobacteria, Proteobacteria and Acidobacteria belong to the dominant groups in rhizosphere soil bacteria.The numbers of fungi and bacteria OTU (Operational taxonomic unit)and Shannon diversity index of altitude 2964 m are higher than the other four sample points (altitude 2373 m, 2608 m, 2733 m, 3280 m).Redundancy analysis showed that the soil microbes in the rhizosphere of S.chamaejasme are different and are affected differently by soil environmental factors.The correlation coefficient showed that the diversity of soil fungi is positively correlated with soil potassium, phosphorus, iron, calcium, molybdenum, altitude, soil moisture, and peroxidase, as well as soil temperature, polyphenol oxidase, dehydrogenase, invertase, and alkaline phosphatase is negatively correlated.Soil bacteria are negatively correlated with soil pH, potassium, phosphorus, magnesium, calcium, and soil moisture, and positively correlated with polyphenol oxidase, peroxidase, urease, dehydrogenase, invertase, acid phosphatase, and alkaline phosphatase.[Conclusion] The composition and diversity of the rhizosphere microbial community of S.chamaejasme in the alpine grasslands of Gansu Province are obviously different.In addition, the physical and chemical properties of soil have a strong positive correlation with soil fungi, and the soil enzymes have more influence on the composition and diversity of soil bacterial communities. |
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来源
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微生物学报
,2021,61(11):3686-3704 【核心库】
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DOI
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10.13343/j.cnki.wsxb.20210109
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关键词
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瑞香狼毒
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根际
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微生物群落结构
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土壤环境因素
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高通量测序
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地址
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兰州大学草地微生物研究中心, 草地农业生态系统国家重点实验室, 甘肃, 兰州, 730000
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中国科学院兰州化学物理研究所, 中国科学院西北特色植物资源化学重点实验室;;甘肃省天然药物重点实验室, 甘肃, 兰州, 730000
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烟台中科先进材料与绿色化工产业技术研究院, 山东, 烟台, 264006
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江苏省南京市海关动植物与食品检测中心, 江苏, 南京, 210000
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0001-6209 |
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基金
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国家自然科学基金
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中国科学院兰州院地合作项目
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中国科学院战略性先导科技专项
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烟台中科先进材料与绿色化工产业技术研究院科技创新项目
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文献收藏号
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CSCD:7086427
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参考文献 共
54
共3页
|
|
1.
Zhang Y H. Chloroplast phylogeny and phylogeography of Stellera chamaejasme on the Qinghai-Tibet Plateau and in adjacent regions.
Molecular Phylogenetics and Evolution,2010,57(3):1162-1172
|
被引
26
次
|
|
|
|
|
2.
尤延飞. 内蒙古天然草地毒草危害状况与防控对策.
动物医学进展,2018,39(4):105-110
|
被引
4
次
|
|
|
|
|
3.
黄祖杰. 草地重要有毒植物-狼毒.
四川草原,1993(4):24-27
|
被引
19
次
|
|
|
|
|
4.
鲍根生. 狼毒斑块对狼毒型退化草地植被和土壤理化性质影响的研究.
草业学报,2019,28(3):51-61
|
被引
20
次
|
|
|
|
|
5.
刘咏梅. 退化高寒草甸狼毒发生区土壤真菌多样性的空间变异.
应用生态学报,2020,31(1):249-258
|
被引
5
次
|
|
|
|
|
6.
吴国林. 青海草地毒草狼毒的发生及防治对策.
青海农林科技,2006(2):63-64
|
被引
7
次
|
|
|
|
|
7.
王福山. 狼毒对西藏高原高寒草甸退化的指示作用.
应用与环境生物学报,2016,22(4):567-572
|
被引
15
次
|
|
|
|
|
8.
Qian S M. Chamaejasmine induces apoptosis in HeLa cells through the PI3K/Akt signaling pathway.
Anti-Cancer Drugs,2017,28(1):40-50
|
被引
1
次
|
|
|
|
|
9.
Kim M. Stellera chamaejasme and its constituents induce cutaneous wound healing and anti-inflammatory activities.
Scientific Reports,2017,7:42490
|
被引
1
次
|
|
|
|
|
10.
Li X Q. Chemical constituents and pharmacological activities of Stellera chamaejasme.
Current Pharmaceutical Design,2018,24(24):2825-2838
|
被引
3
次
|
|
|
|
|
11.
李晓惠. 瑞香狼毒研究进展.
动物医学进展,2019,40(4):96-99
|
被引
6
次
|
|
|
|
|
12.
Jin H. Phylogenic diversity and tissue specificity of fungal endophytes associated with the pharmaceutical plant, Stellera chamaejasme L. revealed by a cultivation-independent approach.
Antonie Van Leeuwenhoek,2015,108(4):835-850
|
被引
2
次
|
|
|
|
|
13.
Jin H. Characterization of rhizosphere and endophytic bacterial communities from leaves, stems and roots of medicinal Stellera chamaejasme L.
Systematic and Applied Microbiology,2014,37(5):376-385
|
被引
12
次
|
|
|
|
|
14.
周攀.
瑞香狼毒对根际微生物群落的影响及在干旱胁迫下狼毒蛋白表达谱的变化. 硕士学位论文,2015
|
被引
2
次
|
|
|
|
|
15.
Jin H. Bacterial community structure associated with the rhizosphere soils and roots of Stellera chamaejasme L. along a Tibetan elevation gradient.
Annals of Microbiology,2018,68(5):273-286
|
被引
4
次
|
|
|
|
|
16.
吴林坤. 根系分泌物介导下植物-土壤-微生物互作关系研究进展与展望.
植物生态学报,2014,38(3):298-310
|
被引
271
次
|
|
|
|
|
17.
Legay N. Contribution of above-and below-ground plant traits to the structure and function of grassland soil microbial communities.
Annals of Botany,2014,114(5):1011-1021
|
被引
11
次
|
|
|
|
|
18.
刘京伟. 植物根际微生物群落构建的研究进展.
微生物学报,2021,61(2):231-248
|
被引
27
次
|
|
|
|
|
19.
余悦.
黄河三角洲原生演替中土壤微生物多样性及其与土壤理化性质关系. 博士学位论文,2012
|
被引
2
次
|
|
|
|
|
20.
燕国辉. 石油污染条件下河口湿地土壤铁的分布特征.
生态学杂志,2020,39(11):3808-3816
|
被引
3
次
|
|
|
|
|
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