一株高效1,4-雄烯二酮降解菌的筛选、鉴定及其降解转化特性研究
Isolation and identification of a highly efficient Androsta-1,4-diene-3,17-dione degrading bacterium and its degradation characteristics
查看参考文献43篇
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文摘
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从养殖场废水中筛选出1株1,4-雄烯二酮(ADD)高效降解菌株ADD3,经过16S rDNA序列同源性分析,鉴定该菌株为芽孢杆菌(Bacillus sp.).通过单因素实验研究了不同实验条件对菌株ADD3降解特性的影响,采用高效液相色谱与质谱联用技术(UPLC-MS/MS)和高效液相色谱与四级杆飞行时间高分辨率质谱联用技术(UPLC-QTof-MS)鉴定转化产物.结果表明:菌株ADD3通过共代谢方式转化ADD,在MH肉汤培养基中降解500 μg·L~(-1) ADD的半衰期为3.6 h;葡萄糖、乙酸钠、蔗糖和淀粉对菌株ADD3降解ADD均有一定的促进作用,其中,最佳碳源为淀粉,最佳浓度为1000 mg·L~(-1);最佳pH和温度分别为7和40 ℃.实验中共鉴定出5种产物,降解机理主要包括加氢还原、脱氢氧化和羟基化,TP1(雄烯二酮)为主要产物.研究结果可为探索微生物转化ADD的机理、优化去除ADD的研究提供参考,并为构造相关工程菌提供一些基础数据. |
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其他语种文摘
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A single strain of Androsta-1,4-diene-3,17-dione(ADD)degrading bacterium was isolated from farm flush water,identified by 16S rDNA sequence and named Bacillus sp.ADD3.The effect of degradation characteristics was studied through a single factor experiment.The biotransformation products were identified using ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)and ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTof-MS).The results show that the Bacillus sp.ADD3 was able to cometabolize ADD,and the half-life(t1/2)was 3.6 h in Mueller-Hinton Broth at a concentration of 500 μg·L~(-1).All the external carbon sources(glucose,sodium acetate,sucrose and starch)could promote the co-metabolism of ADD by Bacillus sp.ADD3.The optimal external carbon source was starch with an optimal concentration of 1000 mg·L~(-1).The optimal pH and temperature for the degradation were 7 and 40 ℃,respectively.Five degradation products were identified,and the possible degradation mechanisms may include hydrogenation,dehydrogenation and hydroxylation.TP1(4-Androstene-3,17-dione)was the main product.The results obtained in this study may provide basic information for understanding the mechanism of biotransformation of ADD,the optimization of ADD removal as well as basic information for the structure of related engineering bacteria. |
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来源
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环境科学学报
,2021,41(3):940-950 【核心库】
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DOI
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10.13671/j.hjkxxb.2020.0473
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关键词
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1,4-雄烯二酮
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芽孢杆菌
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碳源
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降解转化途径
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地址
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华南师范大学环境研究院, 广东省化学品污染与环境安全重点实验室, 广州, 510006
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中国科学院广州地球化学研究所, 有机地球化学国家重点实验室, 广州, 510640
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中国科学院大学, 北京, 100049
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0253-2468 |
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学科
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环境科学基础理论 |
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基金
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国家自然科学基金
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文献收藏号
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CSCD:6932901
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