激光扫描共聚焦荧光显微镜技术及其在地球生物学中的应用
Confocal Laser Scanning Microscopy and its Application in Geobiology
查看参考文献345篇
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文摘
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光学显微镜作为生命科学研究中的必要手段,经过近400年的发展其性能已得到显著的提升。激光扫描共聚焦荧光显微镜成像技术的出现,使得生物体微观三维结构的观察成为可能,与荧光探针技术的结合更是实现了生物样品从定性到原位定量分析的质的飞跃,同时还可提供生物体微观立体的成分结构信息。本文介绍了激光扫描共聚焦显微镜和荧光探针技术的发展现状、原理及应用方案,列举了该技术在地球生物学领域的主要应用。基于此提出了激光扫描共聚焦显微镜成像技术改进的方向,指出多种显微平台技术联用对生命科学的积极效应,最终实现地球生物学的长足发展。 |
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其他语种文摘
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As an essential tool in life science research,the performance of optical microscopy has been remarkably improved over the past 400 years. The emergence of confocal laser scanning microscopy (CLSM) has made it possible to observe the high-resolution three-dimensional microscopic structures of organisms. The combination of CLSM and fluorescent probe technology has achieved the qualitative leap from the qualitative analysis to the quantitative in-situ structural,morphological and compositional characterization of biological samples. Here we have introduced the current states of development, principles and applications of confocal laser scanning microscope and fluorescent probe technology,especially introduced the applications in the field of geobiology. We then have proposed several directions for improving performances of the CLSM and the combination of CLSM with multiple microscopies,to further facilitate their applications in life sciences and to finally advance the development of geobiology. |
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来源
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矿物岩石地球化学通报
,2020,39(6):1141-1172 【核心库】
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DOI
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10.19658/j.issn.1007-2802.2020.39.091
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关键词
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激光扫描共聚焦荧光显微镜
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荧光探针
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地球微生物学
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古生物学
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地址
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中国科学院地球化学研究所, 环境地球化学国家重点实验室, 贵阳, 550081
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中国科学院第四纪科学与全球变化卓越创新中心, 中国科学院第四纪科学与全球变化卓越创新中心, 西安, 710061
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中国科学院地球化学研究所,月球与行星科学研究中心, 贵阳, 550081
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中国科学院比较行星学卓越创新中心, 中国科学院比较行星学卓越创新中心, 合肥, 230026
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1007-2802 |
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学科
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地质学;古生物学 |
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基金
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中国科学院B类战略性先导专项课题
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国家重点研发计划课题
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中国科学院启动经费
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国家自然科学基金项目
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中国科学院贵阳地球化学研究所环境地球化学国家重点实验室基金
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微生物技术国家重点实验室开放课题
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文献收藏号
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CSCD:6885164
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