葡萄籽原花青素对公羔羊生长性能、精液品质及睾丸和附睾抗氧化指标的影响
Effects of Grape Seed Procyanidins on Growth Performance, Semen Quality, Antioxidant Indexes in Testis and Epididymis of Ram
查看参考文献32篇
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文摘
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本试验旨在研究饲喂葡萄籽原花青素(GSPs)对公羔羊生长性能、精液品质及睾丸和附睾抗氧化指标的影响。试验选取4月龄、体重[(22.75±1.20) kg]相近的杜泊×小尾寒羊杂交一代公羔48只,随机分为4组,每组12只羊,在基础饲粮饲喂基础上分别补饲0(对照组)、10(10GSPs组)、20(20GSPs组)、40 mg/kg BW(40GSPs组)的GSPs。试验期共60 d,其中预试期15 d,正试期45 d。结果表明:1) 10GSPs和20GSPs组平均日增重和平均日采食量显著高于对照组和40GSPs组(P<0.05),40GSPs组与对照组差异不显著(P>0.05)。补饲GSPs对料重比影响不显著(P>0.05)。2) GSPs补饲组的附睾重量均显著高于对照组(P<0.05),睾丸、附睾指数在4组间无显著差异(P>0.05)。20GSPs和40GSPs组的顶体完整率显著高于10GSPs组和对照组(P<0.05),精子畸形率显著低于对照组(P<0.05)。3)20GSPs和40GSPs组睾丸、附睾组织中的总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH-Px)活性显著高于对照组(P<0.05), GSPs补饲组附睾超氧化物歧化酶(SOD)活性均显著高于对照组(P<0.05),20GSPs和40GSPs组睾丸、附睾组织中丙二醛(MDA)含量显著低于10GSPs和对照组(P<0.05)。4)睾丸组织中, 20GSPs组的SOD mRNA相对表达量显著高于10GSPs组和对照组(P<0.05),40GSPs组的谷胱甘肽过氧化物酶4(GPx4) mRNA相对表达量显著高于10GSPs组和对照组(P<0.05),4组间的过氧化氢酶(CAT)和核因子E2相关因子2(Nrf2) mRNA相对表达量差异不显著(P>0.05);附睾组织中,20GSPs和40GSPs组SOD mRNA相对表达量显著高于对照组(P<0.05),与10GSPs组差异不显著(P>0.05);GSPs补饲组GPx4 mRNA相对表达量均显著高于对照组(P<0.05); 20GSPs和40GSPs组Nrf2 mRNA相对表达量显著高于对照组(P<0.05)。综上,补饲适量GSPs可以促进绵羊公羔生长,提高睾丸和附睾组织抗氧化能力,进而改善精液品质;在本试验条件下GSPs最适补饲量为20 mg/kg BW。 |
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其他语种文摘
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The aim of this experiment was to investigate the effects of feeding grape seed procyanidins(GSPs) on growth performance, semen quality, testis and epididymis antioxidant indexes of male lambs. A total of forty-eight 4-month-old Dorper (♂)×small Tail Han sheep(♀) F1 male lambs with similarly weight of(22.75± 1.2) kg were randomly selected and divided into 4 groups equally. All the lambs fed a basal diet and supplemented with 0(control group), 10(10GSPs group), 20(20GSPs group) and 40 mg/kg BW(40GSPs group) GSPs, respectively. The experiment was conducted over 60 d, with the first 15 d for adaptation and 45 d for test. The results showed as follows: 1)average daily gain(ADG) and average daily feed intake(AD-FI) in 10GSPs和20GSPs groups were significantly higher than those in the control group and 40GSPs group(P<0.05), with no significant difference between 40GSPs group and control group(P>0.05). No significant difference was observed in feed conversion ratio among GSPs groups(P>0.05). 2) The weight of epididymis in the GSPs groups was significantly higher than that in the control group(P<0.05), and the indexes of testis and epididymis had no significant difference among the four groups(P > 0.05). The acrosome integrity in 20GSPs and 40GSPs groups was significantly higher than that in 10GSPs and control groups(P<0.05), and the rate of sperm malformation in 20GSPs and 40GSPs groups was significantly lower than that in control group(P<0.05). 3) The activity of total antioxidant capacity(T-AOC) and glutathion peroxidase(GSH-Px) in testis and epididymis in 20GSPs and 40GSPs groups were significantly higher than those in control group(P< 0.05). The superoxide dismutase(SOD) activity in epididymis in three GSPs groups was significantly higher than that in the contol group(P<0.05), and the malonaldehyde(MDA) content in the testis and epididymis in 20GSPs and 40GSPs groups was significantly lower than that in 10GSPs group and control group(P<0.05). 4) In testis, the relative expression levels of SOD mRNA in 20GSPs group and glutathione peroxidase 4(GPx4) mRNA in 40GSPs group were significantly higher than those in 10GSPs group and control group(P< 0.05). There was no significant difference among four groups in the relative expression levels of catalase(CAT) and nuclear factor E2 correlation factor 2(Nrf2) mRNA(P>0.05). In epididymis, the relative expression levels of SOD mRNA in 20GSPs and 40GSPs groups was significantly higher than that in control group(P<0.05), but had no significantly difference compared with 10GSPs group(P >0.05). The relative expression level of GPx4 mRNA in three GSPs groups was significantly higher than that in control group(P< 0.05). Additionally, the relative expression level of Nrf2 mRNA in 20GSPs and 40GSPs groups was significantly higher than that in control group(P<0.05). In summary, dietary supplementation of GSPs can promote the growth and improve the antioxidant status of testis and epididymis of ram, thereby improving semen quality. The optimal GSPs supplemental level is 20 mg/kg BW under the condition of this experiment. |
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来源
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动物营养学报
,2020,32(5):2241-2250 【核心库】
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DOI
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10.3969/j.issn.1006-267x.2020.05.033
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关键词
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葡萄籽原花青素
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睾丸
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附睾
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精液品质
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抗氧化功能
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地址
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山西农业大学动物科技学院, 太谷, 030801
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1006-267X |
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学科
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畜牧、动物医学、狩猎、蚕、蜂 |
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基金
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国家重点研发项目
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国家肉羊产业技术体系项目
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山西省基础研究计划项目
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“1331工程”畜牧学重点学科建设计划项目
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文献收藏号
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CSCD:6769040
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