帮助 关于我们

返回检索结果

KAI1 reverses the epithelial-mesenchymal transition in human pancreatic cancer cells

查看参考文献34篇

文摘 Background: Epithelial-mesenchymal transition (EMT) plays an important role in pancreatic cancer (PC). In the present study, we investigated the effects of KAI1 gene overexpression on the EMT of human PC cell lines, MIA PaCa-2 and PACN-1. Methods: Plasmids overexpressing KAI1 and pCMV were transfected into MIA PaCa-2 and PACN-1 cells, respectively. After selection of differently transfected cells by G418, KAI1 protein levels were examined by Western blotting, and transfected cells were renamed as MIA PaCa-2-K, MIA PaCa-2-p, PACN-1-K and PACN-1-p. Wound healing and Transwell migration assays were then performed comparing the two groups of cells. EMT-related markers were analyzed by Western blotting. Results: The percentage of wound closure significantly decreased in MIA PaCa-2-K cells compared with MIA PaCa-2-p and MIA PaCa-2 cells after 24, 48 and 72 h (P < 0.05). In PACN-1-K cells, the percentage of wound closure significantly decreased as well (P < 0.05). Numbers of invading MIA PaCa-2, MIA PaCa-2-p and MIA PaCa-2-K cells were determined as 48.0 ±15.4, 50.0 ±12.4, and 12.0 ±3.8, respectively. The corresponding numbers of invading PACN-1, PACN-1-p and PACN-1-K cells were 29.0 ±10.6, 31.0 ±11.4, and 8.0 ±4.2, respectively. KAI1 overexpression induced a significant upregulation of E-cadherin and also significant downregulation of Snail, vimentin, matrix metalloproteinase 2 (MMP2) and MMP9 (all P < 0.05) in PC cells. Conclusions: KAI1 reversed EMT-related marker expression and inhibited migration and invasion of PC cells. Thus, KAI1 might represent a novel potential therapeutic target for PC.
来源 Hepatobiliary & Pancreatic Diseases International ,2019,18(5):471-477 【核心库】
DOI 10.1016/j.hbpd.2019.03.004
关键词 KAI1 ; Epithelial-mesenchymal transition ; Pancreatic cancer
地址

Department of Gastroenterology, General Hospital of Shenyang Military Area, Shenyang, 110840

语种 英文
文献类型 研究性论文
ISSN 1499-3872
学科 肿瘤学
基金 国家自然科学基金 ;  the Science Foundation of Liaoning
文献收藏号 CSCD:6604826

参考文献 共 34 共2页

1.  Mcguire S. World cancer report 2014. Geneva, Switzerland: world health organization, international agency for research on cancer,2015 被引 4    
2.  Mcguire S. Adv Nutr,2016,7:418-419 被引 85    
3.  Ferlay J. Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer,2015,136:E359–E386 被引 1093    
4.  Lamouille S. Molecular mechanisms of epithelial-mesenchymal transition. Nat Rev Mol Cell Biol,2014,15:178-196 被引 258    
5.  Stone R C. Epithelial-mesenchymal transition in tissue repair and fibrosis. Cell Tissue Res,2016,365:495-506 被引 27    
6.  Jie X X. Epithelial-to-mesenchymal transition, circulating tumor cells and cancer metastasis: mechanisms and clinical applications. Oncotarget,2017,8:81558-81571 被引 11    
7.  Krantz S B. Contribution of epithelial-to-mesenchymal transition and cancer stem cells to pancreatic cancer progression. J Surg Res,2012,173:105-112 被引 9    
8.  Gaianigo N. EMT and treatment resistance in pancreatic cancer. Cancers (Basel),2017,9:122 被引 3    
9.  Beuran M. The epithelial to mesenchymal transition in pancreatic cancer: a systematic review. Pancreatology,2015,15:217-225 被引 8    
10.  Gonzalez D M. Signaling mechanisms of the epithelial-mesenchymal transition. Sci Signal,2014,7:re8 被引 57    
11.  Huang W. Expression of PTEN and KAI1 tumor suppressor genes in pancreatic carcinoma and its association with different pathological factors. Oncol Lett,2016,11:559-562 被引 1    
12.  Miranti C K. Controlling cell surface dynamics and signaling: how CD82/KAI1 suppresses metastasis. Cell Signal,2009,21:196-211 被引 9    
13.  Liu X. KAI1 inhibits lymphangiogenesis and lymphatic metastasis of pancreatic cancer in vivo. Hepatobiliary Pancreat Dis Int,2014,13:87-92 被引 2    
14.  Prieto-Garcia E. Epithelial-to-mesenchymal transition in tumor progression. Med Oncol,2017,34:122 被引 10    
15.  Liu X. KAI1 inhibits HGF-induced invasion of pancreatic cancer by sphingosine kinase activity. Hepatobiliary Pancreat Dis Int,2011,10:201-208 被引 4    
16.  Liu X. Src/STAT3 signaling pathways are involved in KAI1-induced downregulation of VEGF-C expression in pancreatic cancer. Mol Med Rep,2016,13:4774-4778 被引 5    
17.  Wu C Y. Overexpression of KAI1 induces autophagy and increases MiaPaCa-2 cell survival through the phosphorylation of extracellular signal-regulated kinases. Biochem Biophys Res Commun,2011,404:802-808 被引 3    
18.  Wu C Y. Hypoxia and serum deprivation protected MiaPaCa-2 cells from KAI1-induced proliferation inhibition through autophagy pathway activation in solid tumors. Clin Transl Oncol,2015,17:201-208 被引 2    
19.  Cano C E. Epithelial-to-mesenchymal transition in pancreatic adenocarcinoma. Scientific World J,2010,10:1947-1957 被引 7    
20.  Liu X. E-cadherin and gastric cancer: cause, consequence, and applications. Biomed Res Int,2014,2014:637308 被引 5    
引证文献 1

1 余娜 臭牡丹不同提取物的抗肿瘤活性筛选及其对裸鼠移植瘤中EMT相关蛋白的影响 中药药理与临床,2020,36(4):124-131
被引 3

显示所有1篇文献

论文科学数据集
PlumX Metrics
相关文献

 作者相关
 关键词相关
 参考文献相关

版权所有 ©2008 中国科学院文献情报中心 制作维护:中国科学院文献情报中心
地址:北京中关村北四环西路33号 邮政编码:100190 联系电话:(010)82627496 E-mail:cscd@mail.las.ac.cn 京ICP备05002861号-4 | 京公网安备11010802043238号