非水相脂肪酶催化氟比洛芬维C酯的合成及条件优化
Lipase-catalyzed Synthesis of Flurbiprofen VC Ester
查看参考文献12篇
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文摘
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采用非水相酶催化法合成氟比洛芬维生素C酯,使用高效液相色谱和质谱等方法对产物结构进行了表征.同时构建非水相反应体系,通过研究确定最优合成条件:在10 mL、0.057 mol/L的叔丁醇维生素C溶液中,分别添加2.84 mmol氟比洛芬甲酯,10%的N435脂肪酶(以底物的质量分数计),0.8 g分子筛,在70 ℃,200 r/min的条件下反应28 h.最优条件下的产物转化率为73.63%. |
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其他语种文摘
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By using five kinds of organic solvents as reaction medium,the immobilized lipase B from Candida Antarctica (Novozym 435) as the biocatalyst,Lipase-catalyzed synthesis of Flurbiprofen VC ester was studied. Thinlayer chromatogram,high-performance liquid chromatogram and mass spectrum has been used to analyze the structure of the product. The non-aqueous reaction system was established and the reaction condition was optimized. In 10 mL,0.057 mol/L of vitamin C solution of tert-butanol was added 2.84 mmol flurbiprofen ester,the ratio of enzyme to substrate was 5% (W/W),the mount of molecular sieve was 0.8 g,and the temperature was 70 ℃,the rotating speed was 200 r/min,After the reaction 28 h,the maximum conversion rate of the product reaches 73.63%. |
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来源
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分子催化
,2019,33(4):323-330 【扩展库】
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关键词
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脂肪酶
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氟比洛芬维C酯
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非水相反应体系
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前药
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地址
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1.
哈尔滨商业大学, 黑龙江普通高校食品科学与工程重点实验室, 黑龙江, 哈尔滨, 150076
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中国科学院兰州物理化学研究所, 羰基合成与选择氧化国家重点实验室, 甘肃, 兰州, 730000
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1001-3555 |
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学科
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化学工业 |
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基金
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黑龙江省应用技术研究与开发计划项目
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哈尔滨商业大学研究生创新科研项目
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文献收藏号
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CSCD:6587265
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