帮助 关于我们

返回检索结果

A mitochondrial phosphate transporter, McPht gene, confers an acclimation regulation of the transgenic rice to phosphorus deficiency

查看参考文献48篇

Han Jiao 1   Yu Guohong 2   Wang Li 1 *   Li Wei 2   He Rui 3   Wang Bing 2   Huang Shengcai 2   Cheng Xianguo 2 *  
文摘 Phosphate transporters play an important role in promoting the uptake and transport of phosphate in plants. In this study, the McPht gene from the Mesembryanthemum crystallinum, a mitochondrial phosphate transporter, was isolated and constructed onto a constitutive expression vector carrying 35S::GFP, and the recombinant constructs were transferred into Oryza sativa japonica L. cv. Kitaake to investigate the regulatory role of the McPht gene under phosphorus deficiency. The McPht gene encodes a protein of 357 amino acids with six transmembrane domains and is located to the mitochondria, and the mRNA transcripts of the McPht gene are highly accumulated in the shoots of M. crystallinum in response to phosphorus deficiency. However, more mRNA transcripts of the McPht gene were accumulated in the roots of the transgenic rice under phosphorus deficiency. Measurements showed that the transgenic rice demonstrated an enhanced promotion in the root development, the root activities, and phosphate uptake under phosphorus deficiency. Transcriptome sequencing showed that the transgenic rice exhibited total of 198 differentially expressed genes. Of these, total of 154 differentially expressed genes were up-regulated and total 44 genes were down-regulated comparing to the wild type in response to phosphorus deficiency. The selective six genes of the up-regulated differentially expressed genes showed an enhanced increase in mRNA transcripts in response to phosphorus deficiency, however, the transcripts of the mitochondrial carrier protein transporter in rice, a homologous gene of the McPht, in both the transgenic line and the wild type had no obvious differences. Functional enrichment analyses revealed that the most of the up-regulated genes are involved in the cytoplasmic membrane-bounded vesicle, and most of the down-regulated genes are involved in the mitochondrion and cytoplasmic membrane-bounded vesicle. The differentially expressed genes were highly enriched in plant secondary metabolisms and plant-pathogen interaction. These results indicated that the overexpression of the McPht gene might participate in the physiological adaptive modulation of the transgenic rice to phosphorus deficiency by up- or down-regulating the differentially expressed genes.
来源 Journal of Integrative Agriculture ,2018,17(9):1932-1945 【核心库】
DOI 10.1016/S2095-3119(17)61792-1
关键词 McPht gene ; phosphorus deficiency ; phosphate transporter ; transcriptome sequencing ; transgenic rice
地址

1. College of Life Science, Shanxi Normal University, Linfen, 041000  

2. Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081  

3. College of Land and Environment, Shenyang Agricultural University, Shenyang, 110866

语种 英文
文献类型 研究性论文
ISSN 2095-3119
学科 农作物
基金 supported by the National Key Project for Cultivation of New Varieties of Genetically Modified Organisms, Ministry of Agriculture, China ;  国家973计划
文献收藏号 CSCD:6321166

参考文献 共 48 共3页

1.  Adams P. Growth and development of Mesembryanthemum crystallinum (Aizoaceae). New Phytologist,1998,138:171-190 被引 8    
2.  Ai P H. Two rice phosphate transporters, OsPhtl;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocation. The Plant Journal,2009,57:798-809 被引 45    
3.  Bollons H M. Inorganic orthophosphate for diagnosing the phosphorus status of wheat plants. Journal of Plant Nutrition,1997,20:641-655 被引 2    
4.  Bucher M. Molecular and biochemical mechanisms of phosphorus uptake into plants. Journal of Plant Nutrition and Soil Science,2001,164:209-217 被引 10    
5.  Carlos C V. Transcript profiling of Zea mays roots reveals gene responses to phosphate deficiency at the plant and species-specific levels. Journal of Experimental Botany,2008,59:2479-2497 被引 12    
6.  Christine R. Molecular mechanisms of phosphate transport in plants. Planta,2002,216:23-37 被引 66    
7.  Daram P. Functional analysis and cell specific expression of a phosphate transporter from tomato. Planta,1998,206:225-233 被引 33    
8.  Daram P. Pht2;1 encodes a low-affinity phosphate transporter from Arabidopsis. The Plant Cell,1999,11:2153-2166 被引 28    
9.  Duncan R. Turfgrass molecular genetic improvement for abiotic/edaphic stress resistance. Advances in Agronomy,1999,67:233-305 被引 1    
10.  Furihata T. Kinetic characterization of two phosphate uptake systems with different affinities in suspension-cultured Catharanthus roseus protoplasts. Plant and Cell Physiology,1992,33:1151-1157 被引 14    
11.  Grabherr M G. Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nature Biotechnology,2011,29:644-652 被引 730    
12.  Guo B. Functional analysis of the Arabidopsis PHT4 family of intracellular phosphate transporters. New Phytologist,2008,177:889-898 被引 22    
13.  Guo C J. Function of wheat phosphate transporter gene TaPHT2;1 in Pi translocation and plant growth regulation under replete and limited Pi supply conditions. Planta,2013,237:1163-1178 被引 11    
14.  Huang C Y. Metabolite profiling reveals distinct changes in carbon and nitrogen metabolism in phosphate-deficient barley plants (Hordeum vulgare L.). Plant and Cell Physiology,2008,49:691-703 被引 11    
15.  Li Y T. Phosphate transporter OsPht1;8 in rice plays an important role in phosphorus redistribution from source to sink organs and allocation between embryo and endosperm of seeds. Plant Science,2015,230:23-32 被引 9    
16.  Lin W Y. Molecular regulators of phosphate homeostasis in plants. Journal of Experimental Botany,2009,60:1427-1438 被引 24    
17.  Lindstrom A. Seasonal variation in root hardiness in container grown Scots pine, Norway spruce, and Lodgepole pine seedlings. Canadian Journal of Forest Research,1987,17:787-793 被引 6    
18.  Liu C. Tomato phosphate transporter genes are differentially regulated in plant tissues by phosphorus. Plant Physiology,1998,116:91-99 被引 36    
19.  Livak K J. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method. Methods,2001,25:402-408 被引 8316    
20.  Locatelli F. Efficiency of transient transformation in tobacco protoplasts is independent of plasmid amount. Plant Cell Reports,2003,21:865-871 被引 4    
引证文献 1

1 Huang Shengcai Mutagenesis reveals that the rice OsMPT3 gene is an important osmotic regulatory factor The Crop Journal,2020,8(3):465-479
被引 0 次

显示所有1篇文献

论文科学数据集
PlumX Metrics
相关文献

 作者相关
 关键词相关
 参考文献相关

版权所有 ©2008 中国科学院文献情报中心 制作维护:中国科学院文献情报中心
地址:北京中关村北四环西路33号 邮政编码:100190 联系电话:(010)82627496 E-mail:cscd@mail.las.ac.cn 京ICP备05002861号-4 | 京公网安备11010802043238号