国家斑马鱼资源中心的资源、技术和服务建设
Development of resources, technologies and services at the China Zebrafish Resource Center
查看参考文献34篇
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文摘
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随着我国斑马鱼研究群体的日益壮大,对各类斑马鱼研究资源和技术的需求日益增加,国家斑马鱼资源中心(China Zebrafish Resource Center, CZRC,网址:http://zfish.cn)于2012年在中国科学院水生生物研究所成立.目前,CZRC已发展成为国内规模最大的单体斑马鱼养殖系统,建成包含1200多个斑马鱼品系和10 000余份冻存精子的斑马鱼资源保藏库,其中有超过200个突变和转基因品系是由CZRC自主创制.在此基础上,CZRC建立了安全规范的斑马鱼养殖和健康平台、高效的基因操作平台和稳定高效的精子冻存平台.CZRC致力于为国内外从事斑马鱼研究的科研人员提供各类服务,包括提供斑马鱼品系等资源服务、转基因和基因敲除等技术服务、养殖和健康等咨询服务,以及技术培训和学术会议服务等.经过5年的建设,CZRC已成为国际学术界公认的全球三大斑马鱼资源库之一. |
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其他语种文摘
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With the rapid growth of the Chinese zebrafish community, there is an increasing demand for various types of zebrafish-related resources and technologies. The China Zebrafish Resource Center (CZRC, web: http://zfish.cn) was established at the Institute of Hydrobiology (IHB), Chinese Academy of Sciences (CAS) in 2012. Till now, CZRC has built the largest zebrafish aquaculture unit in China, organized a resource bank containing more than 1200 zebrafish lines and more than 10 000 frozen sperm samples, among which over 200 mutant and transgenic lines were generated by CZRC. CZRC has established several technical supporting platforms, such as the zebrafish husbandry and health control program of international standard, a high-efficient gene manipulation technology platform, and a stable and efficient sperm cryopreservation technology platform. The main task of CZRC is to provide different types of services to zebrafish investigators in China and worldwide, such as resource services (e.g. zebrafish lines), technical services (e.g. gene knockout) and transgenic services, consultancy services (e.g. zebrafish husbandry and health consultation), and conference services [e.g. holding regular technical training courses and biennale Chinese Zebrafish Principal Investigator Meeting (CZPM)]. After five years' development, CZRC is now recognized as one of the three major resource centers in the global zebrafish community. |
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来源
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遗传
,2018,40(8):683-692 【核心库】
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DOI
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10.16288/j.yczz.18-151
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关键词
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斑马鱼
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国家中心
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资源
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技术
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服务
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地址
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中国科学院水生生物研究所,国家斑马鱼资源中心, 淡水生态与生物技术国家重点实验室, 武汉, 430072
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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0253-9772 |
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学科
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动物学 |
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基金
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国家自然科学基金项目
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文献收藏号
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CSCD:6305248
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参考文献 共
34
共2页
|
|
1.
Howe K. The zebrafish reference genome sequence and its relationship to the human genome.
Nature,2013,496(7446):498-503
|
被引
132
次
|
|
|
|
|
2.
Pichler F B. Chemical discovery and global gene expression analysis in zebrafish.
Nat Biotechnol,2003,21(8):879-883
|
被引
3
次
|
|
|
|
|
3.
Pott A. Functional genomics in zebrafish as a tool to identify novel antiarrhythmic targets.
Curr Med Chem,2014,21(11):1320-1329
|
被引
2
次
|
|
|
|
|
4.
贾顺姬. 中国斑马鱼研究发展历程及现状.
遗传,2012,34(9):1082-1088
|
被引
16
次
|
|
|
|
|
5.
Xie X W. Growing with the world: rapid development of the zebrafish research in China and the China Zebrafish Resource Center.
Sci China Life Sci,2015,58(4):396-399
|
被引
2
次
|
|
|
|
|
6.
Zhu Z Y. Embryonic and genetic manipulation in fish.
Cell Res,2000,10(1):17-27
|
被引
11
次
|
|
|
|
|
7.
李阔宇. 斑马鱼资源的开发保藏与国家斑马鱼资源中心.
中国实验动物学报,2014,22(6):93-98,105
|
被引
5
次
|
|
|
|
|
8.
Kim S H. Heterozygous inactivation of tsc2 enhances tumorigenesis in p53 mutant zebrafish.
Dis Model Mech,2013,6(4):925-933
|
被引
2
次
|
|
|
|
|
9.
Krovel A V. Expression of a vas::EGFP transgene in primordial germ cells of the zebrafish.
Mech Dev,2002,116(1/2):141-150
|
被引
11
次
|
|
|
|
|
10.
Tiersch T R. Sperm cryopreservation in fish and shellfish.
Soc Reprod Fertil Suppl,2007,65:493-508
|
被引
2
次
|
|
|
|
|
11.
Yang H. Current status of sperm cryopreservation in biomedical research fish models: zebrafish, medaka, and Xiphophorus.
Comp Biochem Physiol C Toxicol Pharmacol,2009,149(2):224-232
|
被引
1
次
|
|
|
|
|
12.
Harvey B. Cryopreservation of zebra fish spermatozoa using methanol.
Can J Zool,1982,60(8):1867-1870
|
被引
8
次
|
|
|
|
|
13.
Khaliq M. Stat3 regulates liver progenitor cell-driven liver regeneration in zebrafish.
Gene Expr,2018
|
被引
1
次
|
|
|
|
|
14.
Ruzicka L. ZFIN, The zebrafish model organism database: Updates and new directions.
Genesis,2015,53(8):498-509
|
被引
2
次
|
|
|
|
|
15.
Kettleborough R N. A systematic genome-wide analysis of zebrafish protein-coding gene function.
Nature,2013,496(7446):494-497
|
被引
23
次
|
|
|
|
|
16.
Varshney G K. A large-scale zebrafish gene knockout resource for the genome-wide study of gene function.
Genome Res,2013,23(4):727-735
|
被引
4
次
|
|
|
|
|
17.
Lee O. Transgenic fish systems and their application in ecotoxicology.
Crit Rev Toxicol,2015,45(2):124-141
|
被引
2
次
|
|
|
|
|
18.
Sertori R. Genome editing in zebrafish: a practical overview.
Brief Funct Genomics,2016,15(4):322-330
|
被引
3
次
|
|
|
|
|
19.
Holder N. Microinjection of DNA, RNA, and protein into the fertilized zebrafish egg for analysis of gene function.
Methods Mol Biol,1999,97:487-490
|
被引
1
次
|
|
|
|
|
20.
Amsterdam A. Transgenes as screening tools to probe and manipulate the zebrafish genome.
Dev Dyn,2005,234(2):255-268
|
被引
3
次
|
|
|
|
|
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