3种沉水植物根际对沉积物中典型氮循环微生物功能基因丰度的影响
Effects of the Rhizospheres of Three Submerged Plants on the Abundances of Functional Genes of Typical Nitrogen Cycle Microorganisms in Sediments
查看参考文献34篇
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文摘
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【目的】硝化—反硝化和厌氧氨氧化是湖泊生态系统重要的脱氮方式,而氮循环微生物的丰度和群落结构的变化能反应湖泊生态环境的情况,作为评判生态修复的重要指标。【方法】本研究通过模拟培养实验、原位悬挂实验和荧光定量qPCR的方法,探讨水蕴草(Egeria densa)、狐尾藻(Myriophyllum verticillatum)和苦草(Vallisneria natans)对根际沉积物中无机态氮含量和氨氧化古菌(ammonia oxidizing archaea,AOA)、好氧氨氧化细菌(aerobic ammonia-oxidizing bacteria,AOB)、反硝化细菌、厌氧氨氧化菌(anaerobic ammonium oxidation,Anammox)数量的影响。【结果】3种沉水植物均能显著降低根际沉积物中的铵态氮含量(P<0.05);狐尾藻和苦草根际沉积物nirS、nirK、nosZ基因拷贝数升高;苦草和狐尾藻AOA amoA拷贝数升高,AOB amoA拷贝数降低;水蕴草根际沉积物nirS、nirK和nosZ功能基因拷贝数均降低,但hzO基因拷贝数显著增加(P<0.05)。【结论】反硝化是狐尾藻和苦草根际沉积物的主要脱氮方式;苦草和狐尾藻对AOA amoA具有选择性,AOA是沉积物中氨氧化过程的主要承担者;厌氧氨氧化过程是水蕴草根际沉积物的主要脱氮方式。 |
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其他语种文摘
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[Purpose]Nitrification-denitrification and anaerobic ammonium oxidation are important denitrification ways in a lake ecosystem.The abundance and community structure of nitrogen circulating microorganisms can reflect the ecological environment of the lake,and it is an important indicator to judge the ecological restoration.[Method]Simulation experiment and field investigation were designed to study the effects of Egeria densa,Myriophyllum verticillatum and Vallisneria natans rhizosphere on the inorganic nitrogen content and the functional gene abundance of several typical nitrogen cycle microorganisms using qPCR method,such as ammonia oxidizing archaea (AOA),aerobic ammonia-oxidizing bacteria (AOB),denitrifying bacteria and anaerobic ammonium oxidation(Anammox).[Result] Three submerged plants could remarkably reduce the ammonia nitrogen in the rhizosphere sediment (P<0.05).The copies of nirS,nirK and nosZ gene were increased in M.verticillatum and V.natans rhizosphere sediments.The copies of AOA amoA were increased but AOB amoA were decreased in M.verticillatum and V.natans rhizosphere sediments.nirS,nirK and nosZ gene copies were decreased,but hzO gene copies were increased significantly in E.densa rhizosphere sediments (P<0.05).[Conclusion]Denitrification was the main way of nitrogen removal in M.verticillatum and V.natans rhizosphere sediments.M.verticillatum and V.natans were selective for AOA amoA,and AOA was the main contributor to the ammoxidation process in sediments.Anaerobic ammonium oxidation process was the main denitrification way of E.densa rhizosphere sediments. |
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来源
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云南农业大学学报
,2018,33(2):314-323 【核心库】
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DOI
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10.12101/j.issn.1004-390X(n).201711015
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关键词
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沉水植物
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沉积物
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无机氮
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氮循环微生物
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地址
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1.
中国科学院水生生物研究所, 湖北, 武汉, 430072
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中国科学院大学, 北京, 100049
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1004-390X |
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学科
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环境污染及其防治 |
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基金
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国家水体污染控制与治理科技重大专项
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文献收藏号
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CSCD:6253545
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