Atomic force microscopy studies on cellular elastic and viscoelastic properties
查看参考文献56篇
文摘
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In this work, a method based on atomic force microscopy(AFM) approach-reside-retract experiments was established to simultaneously quantify the elastic and viscoelastic properties of single cells. First, the elastic and viscoelastic properties of normal breast cells and cancerous breast cells were measured, showing significant differences in Young's modulus and relaxation times between normal and cancerous breast cells. Remarkable differences in cellular topography between normal and cancerous breast cells were also revealed by AFM imaging. Next, the elastic and viscoelasitc properties of three other types of cell lines and primary normal B lymphocytes were measured; results demonstrated the potential of cellular viscoelastic properties in complementing cellular Young's modulus for discerning different states of cells. This research provides a novel way to quantify the mechanical properties of cells by AFM, which allows investigation of the biomechanical behaviors of single cells from multiple aspects. |
来源
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Science China. Life Sciences
,2018,61(1):57-67 【核心库】
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DOI
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10.1007/s11427-016-9041-9
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关键词
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atomic force microscopy
;
cell
;
mechanical properties
;
viscoelastic properties
;
Young's modulus
;
relaxation time
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地址
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1.
Shenyang Institute of Automation, Chinese Academy of Sciences, State Key Laboratory of Robotics, Shenyang, 110016
2.
Department of Industrial and Manufacturing Systems Engineering, The University of Hong Kong, Hong Kong
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语种
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英文 |
文献类型
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研究性论文 |
ISSN
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1674-7305 |
学科
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基础医学 |
基金
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国家自然科学基金
;
the Youth Innovation Promotion Association CAS
;
the CAS FEA International Partnership Program for Creative Research Teams
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文献收藏号
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CSCD:6190092
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