IHB系稀有鮈鲫遗传结构研究
Genetic Structure of IHB Rare Minnow(Gobiocypris rarus)
查看参考文献24篇
|
文摘
|
分析IHB系稀有鮈鲫在繁育过程中的遗传结构变化,旨在鉴定IHB系的遗传质量,为实验动物封闭群的建群和维持以及濒危物种种群保护提供参考和借鉴。IHB系稀有鮈鲫建群原代(P0)为2006年采自四川省汉源县的野生个体,采用最佳避免近交法繁育出F1~F8世代,利用12对微卫星位点对遗传结构进行分析。在IHB系培育过程中,平均观测等位基因数从4.4个降低为4.1个,平均有效等位基因数从4.0个降低为3.0个,平均期望杂合度从0.5878降低到0.5518,平均多态信息含量从0.5509降到0.5293;随着传代的持续,后代与建群原代之间的遗传距离与分化程度有逐渐增大趋势,但相邻世代间的差异逐渐减小;繁育过程中共丢失了6个等位基因,从F5代后所有等位基因频率均无显著变化。经过8代的繁育,IHB系遗传多样性虽略有下降,但仍维持适宜的水平,近交程度得到有效控制,且遗传结构趋于稳定,符合实验动物封闭群遗传质量要求。IHB系可作为稀有鮈鲫地模种群的活体基因库。 |
|
其他语种文摘
|
The rare minnow(Gobiocypris rarus) is a cyprinid species endemic to the upper Yangtze River,characterized by small size,early sexual maturation,consecutive spawning and sensitivity to chemicals.The species has been widely used as a test organism in environmental science,genetics,ichthyopathology and ecotoxicology.However,wild G.rarus populations are threatened by human activities and decreasing rapidly.The IHB rare minnow is a closed laboratory animal colony,the offspring of 50 wild G.rarus specimens collected in Hanyuan County of Sichuan Province in 2006 and bred using methods that prevent inbreeding.In 2014,the ninth generation(F9) of the original parent generation(P0) was obtained.In this study,12 microsatellite loci were used to reveal the genetic structure of the IHB rare minnow from(P0) to(F8),aiming to evaluate the genetic quality of the IHB rare minnow,provide a reference for the construction and maintenance of a closed laboratory animal colony and support efforts to protect the species.As breeding proceeded from generation P0 to F8,the number of alleles per locus and average effective number of alleles per locus decreased,respectively,from 4.4 to 4.1 and 4.0 to 3.0.The average expected heterozygosity and polymorphic information content also decreased,from 0.5878 to 0.5518 and 0.5509 to 0.5293.The largest Nei genetic distance(0.0702) and smallest similarity coefficient(0.9298) were between P0 and F8 and the smallest genetic distance(0.0078) and the largest similarity coefficient(0.9937) were between F7 and F8.These findings indicates that the genetic distance and genetic differentiation(Fst) increased between the original generation and the last generation,but decreased between successive generations.Six allelic genes were lost during development of the IHB colony but no significant changes were found in gene frequencies after the 5th generation.Although genetic diversity declined slightly,a relatively high and acceptable level was maintained in the IHB colony and inbreeding was controlled effectively.Therefore,the genetic structure of IHB colony is stable and the colony meets the genetic quality control requirement for laboratory animals.The IHB colony can be used as a laboratory animal and as an in vivo gene pool for the topotype population of G.rarus. |
|
来源
|
水生态学杂志
,2018,39(1):83-90 【核心库】
|
|
DOI
|
10.15928/j.1674-3075.2018.01.013
|
|
关键词
|
IHB系稀有鮈鲫
;
实验动物封闭群
;
遗传质量控制
;
微卫星DNA标记
;
遗传结构
|
|
地址
|
1.
中国科学院水生生物研究所, 湖北, 武汉, 430072
2.
中国科学院大学, 北京, 100049
|
|
语种
|
中文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
1674-3075 |
|
学科
|
分子生物学 |
|
基金
|
国家科技支撑计划项目
;
湖北省科技支撑计划
|
|
文献收藏号
|
CSCD:6189824
|
参考文献 共
24
共2页
|
|
1.
方喜业.
实验动物质量控制,2008:126-150
|
被引
1
次
|
|
|
|
|
2.
顾党恩. 应用微卫星标记对稀有鮈鲫封闭群建群过程的遗传监测.
水生生物学报,2012,36(2):197-204
|
被引
6
次
|
|
|
|
|
3.
乐佩琦.
中国濒危动物红皮书:鱼类,1998:74-75
|
被引
3
次
|
|
|
|
|
4.
廖莎. 抗草鱼出血病病毒转基因稀有鮈鲫的初步研究.
水生生物学报,2010,34(4):837-842
|
被引
5
次
|
|
|
|
|
5.
鲁立刚. 不同初始基因频率下原位保种群体遗传漂变的计算机模拟.
黑龙江畜牧兽医,2014(3):1-3
|
被引
2
次
|
|
|
|
|
6.
邵燕. 稀有鮈鲫近交系微卫星多态性分析.
水生生物学报,2009,33(4):649-655
|
被引
9
次
|
|
|
|
|
7.
孙德明. 实验用鱼遗传质量控制及标准化.
遗传,2012,34(9):1202-1207
|
被引
3
次
|
|
|
|
|
8.
汤宏斌.
实验动物学,2006:33-35
|
被引
1
次
|
|
|
|
|
9.
王剑伟. 稀有鮈鲫与鱼类实验动物.
鱼类学论文集(第6辑),1997:144-152
|
被引
4
次
|
|
|
|
|
10.
吴本丽. 封闭群稀有鮈鲫对几种常见化学品的敏感性.
中国环境科学,2014,34(4):1059-1066
|
被引
13
次
|
|
|
|
|
11.
武永淑.
HBK-SPF鸭MHC单倍型筛选及其TAP基因的生物信息学分析,2013
|
被引
2
次
|
|
|
|
|
12.
肖兵兵. 应用微卫星DNA标记对BWEL-SPF鸡种群的群体遗传学分析.
中国畜牧兽医,2010,37(9):106-111
|
被引
2
次
|
|
|
|
|
13.
张曼. 微卫星标记在布氏田鼠封闭群遗传结构研究中的应用.
中国比较医学杂志,2015,25(7):34-38
|
被引
1
次
|
|
|
|
|
14.
中华人民共和国国家质量监督检验检疫总局.
实验动物-哺乳类实验动物的遗传质量控制:GB14923-2010,2010
|
被引
1
次
|
|
|
|
|
15.
中华人民共和国环境保护部.
关于发布《中国生物多样性红色名录--脊椎动物卷》的公告,2015
|
被引
1
次
|
|
|
|
|
16.
周永欣. 稀有Qu鲫—一种新的鱼类毒性试验材料.
动物学研究,1995,16(l):59-63
|
被引
28
次
|
|
|
|
|
17.
Aljanabi. Universal and rapid salt-extraction of high quality genomic DNAfor PCR-based techniques.
Nucleic Acids Research,1997,25(22):4692-4693
|
被引
141
次
|
|
|
|
|
18.
Allendorf F W. Genetic drift and the loss of alleles versus heterozygosity.
Zoo Biology,1986,5(5):181-190
|
被引
14
次
|
|
|
|
|
19.
He Yongfeng. Temporal variation in genetic structure of the Chinese rare minnow (Gobiocypris rarus) in its type locality revealed by microsatellite markers.
Biochemical Genetics,2010,48(3/4):312-325
|
被引
5
次
|
|
|
|
|
20.
He Yongfeng. Genetic structure of an endangered endemic fish (Gobiocypris rarus) in the upper Yangtze River.
Biochemical Systematics andEcology,2012,43:214-225
|
被引
2
次
|
|
|
|
|
了解气象卫星更多信息，请访问