两株绿藻响应CO_2浓度变化的生长和生理特性的研究
THE GROWTH AND PHYSIOLOGICAL RESPONSES OF TWO GREEN ALGAE TO THE CHANGE OF CO_2 CONCENTRATION
查看参考文献47篇
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文摘
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以小球藻FACHB-1580和栅藻FACHB-1618为研究对象,比较了两株绿藻在0.04% CO_2、5% CO_2和20% CO_2 (v/v)三种通气培养条件下的生长和生理特性的响应,试图阐述与无机碳利用相关生理参数和微藻利用CO_2能力的关系。结果表明,两株绿藻均能高效利用CO_2,在5%(v/v)条件下均表现出最大生物量积累、最大比生长速率和最大二氧化碳固定速率。小球藻FACHB-1580和栅藻FACHB-1618最大生物量分别为3.5和5.4 g/L,分别是0.04% CO_2 (v/v)条件的1.41和1.46倍。在高达20% CO_2 (v/v)条件下,两株绿藻的生物量均显著高于空气组(P<0.05)。随着CO_2浓度的增加,两株绿藻的无机碳亲和力、胞内和胞外CA活性、初始Rubisco活性,及Rubisco活化度均有下降趋势,总的Rubisco活性变化不明显。另外,小球藻FACHB-1580存在较高的胞外和胞内CA活性;而栅藻FACHB-1618胞外CA活性几乎为零,胞内CA活性显著低于小球藻FACHB-1580。由此推测,小球藻FACHB-1580能同时吸收介质中的HCO_3~-和CO_2,其胞内CA催化胞内HCO_3~-快速转化为CO_2,从而为Rubisco提供充足的CO_2来源;而栅藻FACHB-1618主要吸收介质中的CO_2,其胞内CA活性较低,推测其通过提高胞内CA含量,或增强Rubisco对CO_2的亲和力等促进光合固碳作用。 |
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其他语种文摘
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Selection of microalgae strains that are capable of utilizing high concentration of CO_2 is essential for industrial application.At present,the microalgae research mainly focus on the biomass,bioactive products and recovery properties,rather than physiological properties.The influence of different CO_2 concentrations,including air,5% CO_2 and 20% CO_2 (v/v),on the growth and physiological properties of Chlorella sp.FACHB-1580 and Scenedesmus sp.FACHB-1618 were studied.The goal was to clarify the relationship between the physiological properties and the CO_2 utilizing capacity.Results showed that both strains were capable to use high concentration of CO_2 and had the maximum biomass accumulation,specific growth rate and CO_2 fixation rate under 5% CO_2 (v/v) culture condition.The maximum biomass was 3.5 g/L in Chlorella sp.FACHB-1580 and 5.4 g/L in Scenedesmus sp.FACHB-1618 which was 1.41 and 1.46 times higher in comparison with the control (air) group,respectively.Both strains had higher biomass under 20% CO_2 (v/v) condition than that in the air group (P < 0.05).With the increasing supply of CO_2,the affinity of inorganic carbon,the activity of intracellular (CA_(int)) and extracellular (CA_(ext)) carbonic anhydrase,as well as the initial activity and activation rate of Rubisco declined in both strains.However,there was no significant difference in the total Rubisco activity across time.In addition,Chlorella sp.FACHB-1580 had significantly higher levels of CA_(int) and CA_(ext) activities.By contrast,Scenedesmus sp.FACHB-1618 had very poor CA_(int) and CA_(ext) activities and were not detectable,indicating these two strains differed significantly in the utility of inorganic carbon.It was speculated that Chlorella sp.FACHB-1580 can absorb both CO_2 and whereas the Scenedesmus sp.FACHB-1618 tends to only absorb CO_2.Chlorella sp.FACHB-1580 may obtain its abundant CO_2 for Rubisco via high CA_(int) activity;for Scenedesmus sp.FACHB-1618,due to its lower CA_(int) activity,it may increase the amount of CA protein,or higher carboxylase affinity for CO_2 to promote the reactions of photosynthesis. |
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来源
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水生生物学报
,2018,42(1):182-189 【核心库】
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DOI
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10.7541/2018.023
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关键词
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绿藻
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生物量
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二氧化碳浓缩机制(CCM)
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碳酸酐酶(CA)
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1,5-二磷酸核酮糖羧化酶(Rubisco)
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地址
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1.
中国科学院水生生物研究所, 中国科学院藻类生物学重点实验室, 武汉, 430072
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中国科学院大学, 北京, 100049
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1000-3207 |
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学科
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植物学 |
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基金
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国家863计划
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文献收藏号
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CSCD:6155890
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参考文献 共
47
共3页
|
|
1.
Chisti Y. Biodiesel from microalgae.
Biotechnology Advances,2007,25(3):294-306
|
被引
304
次
|
|
|
|
|
2.
Rahaman M S A. A review of carbon dioxide capture and utilization by membrane integrated microalgal cultivation processes.
Renewable and Sustainable Energy Reviews,2011,15(8):4002-4012
|
被引
4
次
|
|
|
|
|
3.
De Morais M G. Biofixation of carbon dioxide by SpirμLina sp. and Scenedesmus obliquus cultivated in a threestage serial tubμLar photobioreactor.
Journal of Biotechnology,2007,129(3):439-445
|
被引
27
次
|
|
|
|
|
4.
Chiu S Y. Reduction of CO_2 by a high-density culture of Chlorella sp. in a semicontinuous photobioreactor.
Bioresource Technology,2008,99(9):3389-3396
|
被引
25
次
|
|
|
|
|
5.
Forster B. Very high light resistant mutants of Chlamydomonas reinhardtii: responses of photosystemⅡ, nonphotochemical quenching and xanthophyll pigments to light and CO_2.
Photosynthesis Research,2001,67(1/2):5-15
|
被引
1
次
|
|
|
|
|
6.
Moroney J V. Proposed carbon dioxide concentrating mechanism in Chlamydomonas reinhardtii.
Eukaryotic Cell,2007,6(8):1251-1259
|
被引
6
次
|
|
|
|
|
7.
Gerloff E A. Effect of external pH on the growth, photosynthesis and photosynthetic electron transport of Chlamydomonas acidophila Negoro, isolated from an extremely acidic lake (pH 2.6).
Plant, Cell & Environment,2005,28(10):1218-1229
|
被引
3
次
|
|
|
|
|
8.
Spijkerman E. CO_2 acquisition in Chlamydomonas acidophila is influenced mainly by CO_2, not phosphorus, availability.
Photosynthesis Research,2014,121(2/3):213-221
|
被引
1
次
|
|
|
|
|
9.
Tsuzuki M. CO_2 syndrome in Chlorella.
Canadian Journal of Botany,1991,69(5):1003-1007
|
被引
1
次
|
|
|
|
|
10.
Moroney J V. Evidence for inorganic carbon transport by intact chloroplasts of Chlamydomonas reinhardtii.
Plant Physiology,1987,83(3):460-463
|
被引
1
次
|
|
|
|
|
11.
Ashida H. Was photosynthetic Rubisco recruited by acquisitive evolution from Rubisco-like proteins involved in sulfur metabolism?.
Research in Microbiology,2005,156(5/6):611-618
|
被引
16
次
|
|
|
|
|
12.
Bowes G. Growth at elevated CO_2: photosynthetic responses mediated through Rubisco.
Plant, Cell & Environment,1991,14(8):795-806
|
被引
16
次
|
|
|
|
|
13.
Spijkerman E. What physiological acclimation supports increased growth at high CO_2 conditions.
Physiologia Plantarum,2008,133(1):41-48
|
被引
1
次
|
|
|
|
|
14.
Price G D. Association of carbonic anhydrase activity with carboxysomes isolated from the cyanobacterium Synechococcus PCC7942.
Plant Physiology,1992,100(2):784-793
|
被引
4
次
|
|
|
|
|
15.
Rogers A. Photosynthetic acclimation of Pinus taeda (loblolly pine) to long-term growth in elevated pCO_2 (FACE).
Plant, Cell & Environment,2002,25(7):851-858
|
被引
8
次
|
|
|
|
|
16.
Rippka R. Generic assignments, strain histories and properties of pure cultures of cyanobacteria.
Microbiology,1979,111(1):1-61
|
被引
153
次
|
|
|
|
|
17.
Qiu B S. Effects of CO_2 enrichment on the bloom-forming cyanobacterium Microcystis aeruginosa (cyanophyceae): Physiological responses and relationships with the availability of dissolved inorganic carbon1.
Journal of Phycology,2002,38(4):721-729
|
被引
22
次
|
|
|
|
|
18.
Rotatore C. The acquisition and accumulation of inorganic carbon by the unicellular green alga Chlorella ellipsoidea.
Plant, Cell & Environment,1991,14(4):377-382
|
被引
6
次
|
|
|
|
|
19.
Mouget J L. Inadequacy of Rubisco initial and total activities to account for observed rates of photosynthetic carbon dioxide assimilation by Scenedesmus ecornis.
European Journal of Phycology,1993,28(2):99-106
|
被引
2
次
|
|
|
|
|
20.
Gerard V A. A spectrophotometric assay for Rubisco activity: application to the kelp laminaria saccharrina and implications for radiometric assays.
Journal of Phycology,1996,32(5):880-884
|
被引
10
次
|
|
|
|
|
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