不同体积分数氧气对山羊颞下颌关节盘细胞骨架改建的影响
Effect of different oxygen tension on the cytoskeleton remodeling of goat temporomandibular joint disc cells
查看参考文献26篇
文摘
|
目的探讨不同体积分数的氧气对山羊颞下颌关节盘细胞3种细胞骨架蛋白改建的影响。方法体外分离、培养山羊颞下颌关节盘细胞,传代至第2代,分别于常氧21%O_2及低氧8%O_2、4%O_2、2%O_2下培养。甲苯胺蓝、天狼星红及Ⅰ型胶原免疫细胞化学染色观察不同氧体积分数下细胞显型的变化。细胞免疫荧光染色和实时定量逆转录聚合酶链反应检测细胞骨架蛋白,包括肌动蛋白、微管蛋白和波形蛋白的表达情况。结果不同氧体积分数下关节盘细胞仍然具有成纤维特性,3种细胞骨架蛋白有规律地排列。4%O_2时,肌动蛋白和波形蛋白荧光强度最低(P<0.05);2%O_2时,微管蛋白荧光强度最高(P<0.05),其他各组间差异无统计学意义(P>0.05)。检测肌动蛋白mRNA表达,21%O_2组最高,而2%O_2组和4%O_2组最低(P<0.05);微管蛋白mRNA表达在2%O_2组最高,8%O_2组最低(P<0.05);波形蛋白mRNA表达,在4%O_2组最低,21%O_2组最高,差异有统计学意义(P<0.05)。结论在不同氧体积分数条件下,细胞骨架蛋白有不同程度的改建,2%O_2可能是适合颞下颌关节盘细胞扩增的最佳氧体积分数。 |
其他语种文摘
|
Objective The effect of different oxygen tensions on the cytoskeleton remodeling of goat temporomandibular joint (TMJ) disc cells were investigated. Methods Goat TMJ disc cells were cultured under normoxia (21% O_2) and hypoxia (2%, 4%, and 8% O_2). Toluidine blue, picrosirius red, and type Ⅰ collagen immunocytochemical staining were performed to observe the changes in cell phenotype under different oxygen levels. Immunofluorescent staining and real-time reverse transcription- polymerase chain reaction analysis were then performed to identify actin, tubulin, and vimentin in the cultured disc cells. Results TMJ disc cells still displayed fibroblast characteristics under different oxygen levels and their cytoskeletons had regular arrangement. The fluorescence intensities of actin and vimentin were lowest at 4% O_2 (P<0.05), whereas that of tubulin was highest at 2% O_2 (P<0.05). No significant difference among the other groups was observed (P>0.05). Actin mRNA levels were considerably decreased at 2% O_2 and 4% O_2 in hypoxic conditions, while actin mRNA expression was highest in 21% O_2. Tubulin mRNA levels considerably increased at 2% O_2, while tubulin mRNA expression was lowest in 8% O_2 (P<0.05). Vimentin mRNA expression was lowest at 4% O_2 and highest at 21% O_2, and significant differences were observed between vimentin mRNA expression levels among these oxygen levels (P<0.05). Conclusion Cytoskeletons were reconstructed in different oxygen tensions, and 2% O_2 may be the optimal oxygen level required to proliferate TMJ disc cells. |
来源
|
华西口腔医学杂志
,2017,35(4):362-367 【核心库】
|
DOI
|
10.7518/hxkq.2017.04.004
|
关键词
|
颞下颌关节盘
;
低氧
;
细胞骨架
;
肌动蛋白
;
微管蛋白
;
波形蛋白
|
地址
|
1.
兰州大学口腔医学研究所, 兰州, 730000
2.
兰州大学口腔医学研究所, 甘肃省口腔疾病研究重点实验室;;口腔医学国家民委重点实验室, 兰州, 730030
|
语种
|
中文 |
文献类型
|
研究性论文 |
ISSN
|
1000-1182 |
学科
|
口腔科学 |
基金
|
国家自然科学基金
|
文献收藏号
|
CSCD:6055218
|
参考文献 共
26
共2页
|
1.
Stankovic S. Morphological and biomechanical features of the temporomandibular joint disc: an overview of recent findings.
Arch Oral Biol,2013,58(10):1475-1482
|
被引
3
次
|
|
|
|
2.
Piette E. Anatomy of the human temporomandibular joint. An updated comprehensive review.
Acta Stomatol Belg,1993,90(2):103-127
|
被引
1
次
|
|
|
|
3.
Benjamin M. Biology of fibrocartilage cells.
Int Rev Cytol,2004,233:1-45
|
被引
6
次
|
|
|
|
4.
Alonso L G. Ultrastructural evidence of vascularization of the central zone of the temporomandibular joint disc in human fetus.
Eur J Anat,2016,10(2):45-48
|
被引
1
次
|
|
|
|
5.
Yamaguchi A. Role of hypoxia and interleukin-1beta in gene expressions of matrix metalloproteinases in temporomandibular joint disc cells.
Arch Oral Biol,2005,50(1):81-87
|
被引
1
次
|
|
|
|
6.
Chisnoiu A M. Factors involved in the etiology of temporomandibular disorders-a literature review.
Clujul Med,2015,88(4):473-478
|
被引
2
次
|
|
|
|
7.
DuRaine G D. Emergence of scaffoldfree approaches for tissue engineering musculoskeletal cartilages.
Ann Biomed Eng,2015,43(3):543-554
|
被引
1
次
|
|
|
|
8.
Shu W. Tissue engineering of the temporomandibular joint disc: current status and future trends.
Int J Artif Organs,2015,38(2):55-68
|
被引
4
次
|
|
|
|
9.
Hsieh C H. Surface ultrastructure and mechanical property of human chondrocyte revealed by atomic force microscopy.
Osteoarthr Cartil,2008,16(4):480-488
|
被引
13
次
|
|
|
|
10.
Lee J K. Initiation of chondrocyte self-assembly requires an intact cytoskeletal network.
Tissue Eng Part A,2016,22(3/4):318-325
|
被引
2
次
|
|
|
|
11.
Kuo J. Regional cell density distribution and oxygen consumption rates in porcine TMJ discs: an explant study.
Osteoarthr Cartil,2011,19(7):911-918
|
被引
3
次
|
|
|
|
12.
Cisewski S E. The effects of oxygen level and glucose concentration on the metabolism of porcine TMJ disc cells.
Osteoarthr Cartil,2015,23(10):1790-1796
|
被引
3
次
|
|
|
|
13.
Cernanec J. Influence of hypoxia and reoxygenation on cytokine-induced production of proinflammatory mediators in articular cartilage.
Arthritis Rheum,2002,46(4):968-975
|
被引
1
次
|
|
|
|
14.
Blain E J. Involvement of the cytoskeletal elements in articular cartilage homeostasis and pathology.
Int J Exp Pathol,2009,90(1):1-15
|
被引
13
次
|
|
|
|
15.
Benjamin M. Cytoskeleton of cartilage cells.
Microsc Res Tech,1994,28(5):372-377
|
被引
4
次
|
|
|
|
16.
Chen C. Effects of vimentin disruption on the mechanoresponses of articular chondrocyte.
Biochem Biophys Res Commun,2016,469(1):132-137
|
被引
2
次
|
|
|
|
17.
Jin H. Resveratrol protects chondrocytes from apoptosis via altering the ultrastructural and biomechanical properties: an AFM study.
PLoS ONE,2014,9(3):e91611
|
被引
2
次
|
|
|
|
18.
Leonardi R. Immunolocalization of vimentin and alpha-smooth muscle actin in dysfunctional human temporomandibular joint disc samples.
J Oral Rehabil,2002,29(3):282-286
|
被引
1
次
|
|
|
|
19.
Thyberg J. Role of microtubules in the organization of the Golgi complex.
Exp Cell Res,1999,246(2):263-279
|
被引
7
次
|
|
|
|
20.
郭恒. 微管蛋白破坏对软骨细胞代谢功能的影响.
中华医学杂志,2011,91(15):1036-1040
|
被引
2
次
|
|
|
|
|