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Comparative transcriptome analysis of shortened fruit mutant in woodland strawberry (Fragaria vesca) using RNA-Seq

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文摘 Genes controlling fruit appearance determine fruit shape and size. In ethylmethane sulfonate (EMS)-mutagenized lines of Fragaria vesca accession Yellow Wonder (YW), two fruit shapes are observed: wild-type long fruit and mutated shortened fruit (sf). In this study, we first characterized sf based on morphology, histology, cytology and physiology. The sf was identified as a gibberellin (GA)-deficient mutant, and four complementary DNA (cDNA) libraries separately constructed from flower buds and small green fruits of YW and sf were sequenced to comparatively analyze transcriptome differences. A total of 29 differentially expressed GA pathway genes were identified by comparisons between YW1 and sf1, and 28 differentially expressed GA pathway genes were identified between YW2 and sf 2. In addition, the expression patterns of 45 differentially expressed genes were validated by quantificational real-time PCR (qRT-PCR), and the results were highly concordant with the RNA-Seq results. This transcriptome analysis provides valuable information for understanding the molecular mechanisms of fruit development of strawberry.
来源 Journal of Integrative Agriculture ,2017,16(4):828-844 【核心库】
DOI 10.1016/S2095-3119(16)61448-X
关键词 strawberry ; fruit shape ; transcriptome ; RNA-Seq ; differentially expressed gene
地址

College of Horticulture, Shenyang Agricultural University, Shenyang, 110866

语种 英文
文献类型 研究性论文
ISSN 2095-3119
学科 园艺
基金 国家自然科学基金
文献收藏号 CSCD:5962859

参考文献 共 60 共3页

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