氮肥添加对高寒藏嵩草(Kobresia tibetica)沼泽化草甸和土壤微生物群落的影响
Effects of N addition on the plant and soil microbial community in alpine Kobresia tibetica meadow of Qinghai-Tibet Plateau
查看参考文献65篇
文摘
|
以藏嵩草沼泽化草甸为研究对象,利用磷脂脂肪酸(PLFA)技术,研究连续6年N素添加对地上植被群落数量特征、土壤微生物群落结构的影响。结果表明: ①藏嵩草沼泽化草甸群落生物量、枯枝落叶对施肥处理无明显响应,且莎草科植物对土壤氮素的吸收和利用率较低。②施肥增加了0—10 cm土壤微生物类群PLFAs丰富度,尤其细菌和革兰氏阳性菌PLFAs,降低了10—20 cm PLFAs丰富度; ③磷脂脂肪酸饱和脂肪酸/单烯不饱和脂肪酸、细菌PLFAs /真菌PLFAs的比值随土壤层次增加而增加; ④ 0—10 cm土层,革兰氏阳性菌、真菌PLFAs含量与pH、土壤速效磷、速效氮、土壤有机质显著正相关(P<0.05或P< 0.01); 10—20 cm土层,细菌、革兰氏阳性菌、真菌和总PLFAs含量与土壤有机质含量显著正相关(P<0.05或P<0.01) 。表明藏嵩草沼泽化草甸微生物PLFAs含量和丰富度对施肥的响应存在明显的土层梯度效应,土壤微生物PLFAs含量和丰富度主要受表层土壤初始养分含量的影响。 |
其他语种文摘
|
Effects of continuous six-year N addition on the quantitative properties of plant and soil microbial communities in alpine Kobresia tibetica meadows were investigated experimentally by using field surveys and phospholipid fatty acid (PLFA) analyses. The experiment included four manipulative treatments involving both N and litter: added N (+N) with the litter removed (LR),+N with the litter left intact (LI),LI without N addition (-N),and LR with -N. Soil samples were collected at the depths of 0—10 and 10—20 cm. Aboveground biomass (AGB),litter biomass,microbial community composition,and nutrient content were measured. The results showed that (1) fertilization did not have significant effects on AGB and litter biomass. (2) N addition significantly increased the abundance of the microbial group PLFAs at the soil depth of 0—10 cm,particularly the abundance of the bacterial PLFAs (B PLFAs) and gram-positive bacterial PLFAs (G+PLFAs),but decreased microbial abundances at the soil depth of 10—20 cm. (3) The ratio of the normal saturated fatty acids /monounsaturated fatty acids and B PLFAs /fungal PLFAs (F PLFAs) increased with the increase in the soil depth. (4) G+ PLFA and F PLFA contents were significantly and positively correlated to pH,available phosphorus,available nitrogen,and soil organic matter contents at 0—10 cm soil depth. The contents and abundance of fatty acids were mainly regulated and affected by initial nutrient level in the topsoil after short-term N addition. These results indicate that microbial communities in this alpine tundra are sensitive to both litter and N inputs,and that the vertical structures of these communities are particularly vulnerable. |
来源
|
生态学报
,2017,37(2):405-415 【核心库】
|
DOI
|
10.5846/stxb201508041642
|
关键词
|
藏嵩草草甸
;
磷脂脂肪酸(PLFA)
;
微生物群落
;
氮添加
|
地址
|
1.
西南民族大学生命科学与技术学院, 成都, 610041
2.
中国科学院水利部成都山地灾害与环境研究所, 成都, 610041
3.
中国科学院成都生物所, 成都, 610041
|
语种
|
中文 |
文献类型
|
研究性论文 |
ISSN
|
1000-0933 |
学科
|
畜牧、动物医学、狩猎、蚕、蜂 |
基金
|
国家重点基础研究发展计划(973计划)
;
国家自然科学基金项目
;
中央高校基本科研业务费优秀科研团队及重大孵化项目
|
文献收藏号
|
CSCD:5914422
|
参考文献 共
65
共4页
|
1.
Hillebrand H. Consumer versus resource control of producer diversity depends on ecosystem type and producer community structure.
Proceedings of the National Academy of Sciences,2007,104(26):10904-10909
|
被引
10
次
|
|
|
|
2.
李禄军. 氮素添加对科尔沁沙质草地物种多样性和生产力的影响.
应用生态学报,2009,20(8):1838-1844
|
被引
41
次
|
|
|
|
3.
Wang C T. Fertilization and litter effects on the functional group biomass,species diversity of plants,microbial biomass,and enzyme activity of two alpine meadow communities.
Plant and Soil,2010,331(1/2):377-389
|
被引
27
次
|
|
|
|
4.
杨中领.
青藏高原东部高寒草甸群落结构和功能对施肥和放牧的响应,2011
|
被引
2
次
|
|
|
|
5.
杨晓霞. 青藏高原高寒草甸植物群落生物量对氮、磷添加的响应.
植物生态学报,2014,38(2):159-166
|
被引
99
次
|
|
|
|
6.
Printice I C.
The carbon cycle and atmospheric carbon dioxide. IPCC Third Assessment Report,2001:184-237
|
被引
1
次
|
|
|
|
7.
沈菊培. 微生物介导的碳氮循环过程对全球气候变化的响应.
生态学报,2011,31(11):2957-2967
|
被引
46
次
|
|
|
|
8.
何亚婷. 草地生态系统土壤微生物量及其影响因子研究进展.
地理科学进展,2010,29(11):1350-1359
|
被引
35
次
|
|
|
|
9.
Compton J E. Response of soil microbial biomass and community composition to chronic nitrogen additions at Harvard forest.
Forest Ecology and Management,2004,196(1):143-158
|
被引
91
次
|
|
|
|
10.
Wallenstein M D. Nitrogen fertilization decreases forest soil fungal and bacterial biomass in three long-term experiments.
Forest Ecology and Management,2006,222(1/3):459-468
|
被引
50
次
|
|
|
|
11.
Clegg C D. The impact of grassland management regime on the community structure of selected bacterial groups in soils.
FEMS Microbiology Ecology,2003,43(2):263-270
|
被引
11
次
|
|
|
|
12.
莫良玉. 高等植物对有机氮吸收与利用研究进展.
生态学报,2002,22(1):118-124
|
被引
16
次
|
|
|
|
13.
Nasholm T. Uptake of organic nitrogen by plants.
New Phytologist,2009,182(1):31-48
|
被引
55
次
|
|
|
|
14.
王文颖. 植物吸收利用有机氮营养研究进展.
应用生态学报,2009,20(5):1223-1228
|
被引
16
次
|
|
|
|
15.
徐兴良. 植物吸收土壤有机氮的研究进展.
自然资源学报,2011,26(4):715-724
|
被引
18
次
|
|
|
|
16.
Xu X L. Uptake of organic nitrogen by eight dominant plant species in Kobresia meadows.
Nutrient Cycling in Agroecosystems,2004,69(1):5-10
|
被引
10
次
|
|
|
|
17.
Nordin A. Nitrogen uptake by arctic soil microbes and plants in relation to soil nitrogen supply.
Ecology,2004,85(4):955-962
|
被引
14
次
|
|
|
|
18.
Miller A E. Plant uptake of inorganic and organic nitrogen: Neighbor identity matters.
Ecology,2007,88(7):1832-1840
|
被引
18
次
|
|
|
|
19.
Henry H A L. Plant aminoacid,soluble N turnover and microbial N capture in soils of a grazed Arctic saltmarsh.
Journal of Ecology,2003,91(4):627-636
|
被引
10
次
|
|
|
|
20.
曹广民. 土壤-牧草氮素供需状况变化对高寒草甸植被演替与草地退化的影响.
生态学杂志,2004,23(6):25-28
|
被引
37
次
|
|
|
|
|