Analysis of the diversity and function of the alleles of the rice blast resistance genes Piz-t, Pita and Pik in 24 rice cultivars
查看参考文献28篇
|
文摘
|
Understanding the sequence diversity of rice blast resistance genes is important for breeding new resistant rice cultivars against the rice blast fungus Magnaporthe oryzae. In this study, we selected 24 rice cultivars with different genetic backgrounds to study the allelic diversity of rice blast resistance genes Piz-t, Pita and Pik. For Piz-t, a total of 17 allelic types were found within the 24 cultivars. Blast inoculations showed that most of the mutations can affect the function of the resistance gene. For Pita, except for the difference at the 918th amino acid, a majority of the 21 mutations were detected among the cultivars. Inoculations with blast isolates carrying Avr-Pita revealed that cultivars with mutations in other sites except for the 918th amino acid did not affect the function of the Pita gene. For Pik, a total of six allelic types were found within the 24 cultivars, but five of them lost the function of the resistance gene. In addition, we found that Piz-t, Pita and Pik were expressed constitutively in the 24 rice cultivars and the expression level was not related to resistance. Our results have provided the sequence diversity information of the resistance genes Piz-t, Pita and Pik among the popular rice cultivars grown in the northeast region of China. |
|
来源
|
Journal of Integrative Agriculture
,2016,15(7):1423-1431 【核心库】
|
|
DOI
|
10.1016/S2095-3119(15)61207-2
|
|
关键词
|
resistance gene
;
avirulence gene
;
alleles
;
function
;
genetic evolution
|
|
地址
|
1.
Plant Protection College, Shenyang Agricultural University, Shenyang, 110866
2.
Agricultural Crops Molecular Improving Laboratory, Liaoning Academy of Agricultural Sciences, Shenyang, 110161
3.
Department of Plant Pathology, China Agricultural University, Beijing, 100193
|
|
语种
|
英文 |
|
文献类型
|
研究性论文 |
|
ISSN
|
2095-3119 |
|
学科
|
植物保护 |
|
基金
|
国家自然科学基金
;
辽宁省自然科学基金
;
the Doctoral Fund of Liaoning Province of China
|
|
文献收藏号
|
CSCD:5755906
|
参考文献 共
28
共2页
|
|
1.
Altschul S F. Basic local alignment search tool.
Journal of Molecular Biology,1990,215:403-410
|
被引
828
次
|
|
|
|
|
2.
Altschul S F. Gapped BLAST and PSI-BLAST: A new generation ofprotein database search programs.
Nucleic Acids Research,1997,25:3389-3402
|
被引
1186
次
|
|
|
|
|
3.
Ashikawa I. Two adjacent nucleotide-binding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance.
Genetics,2008,180:2267-2276
|
被引
100
次
|
|
|
|
|
4.
Ashkani S. Allele mining strategies: Principles and utilisation for blast resistance genes in rice (Oryza sativa L.).
Current Issues in Molecular Biology,2015,17:57-74
|
被引
7
次
|
|
|
|
|
5.
Bonman J M. Breeding rice for resistance to pests.
Annual Review of Phytopathology,1992,30:507-528
|
被引
25
次
|
|
|
|
|
6.
Bryan G T. A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pita.
The Plant Cell,2000,12:2033-2045
|
被引
195
次
|
|
|
|
|
7.
Chuma I. Multiple translocation of the Avr-Pita effector gene among chromosomes of the rice blast fungus Magnaporthe oryzae and related species.
PLoS Pathogen,2011,7:e1002147
|
被引
20
次
|
|
|
|
|
8.
Costanzo S. Sequence variation at the rice blast resistance gene Pikm locus: Implications for the development of allele specific markers.
Plant Science,2010,178:523-530
|
被引
28
次
|
|
|
|
|
9.
Gish W. Identification of protein coding regions by database similarity search.
Nature Genetics,1993,3:266-272
|
被引
20
次
|
|
|
|
|
10.
Hayashi N. Protocols for the sampling of diseased specimens and evaluation of blast disease in rice.
Japan International Research Center for Agricultural Sciences Working Report,2009,63:17-33
|
被引
2
次
|
|
|
|
|
11.
Jiang N. Analysis of the antimicrobial spectrum of three rice blast resistance genes at Pi2/9 locus and genetic diversity of rice blast strains.
Journal of Hunan Agricultural University. (in Chinese),2012,38:506-510
|
被引
1
次
|
|
|
|
|
12.
Jia Y L. Direct interaction of resistance gene and avirulence gene products confers rice blast resistance.
The EMBO Journal,2000,19:4004-4014
|
被引
29
次
|
|
|
|
|
13.
Kanzaki H. Arms race co-evolution of Magnaporthe oryzae Avr-Pik and rice Pik genes driven by their physical interactions.
The Plant Journal,2012,72:894-907
|
被引
33
次
|
|
|
|
|
14.
Kiyosawa S. Genetics of blast resistance.
Rice Breeding,1972:203-225
|
被引
1
次
|
|
|
|
|
15.
Li J B. Effectiveness and durability of the rice Pita gene in yunnan province of China.
Phytopathology,2014,104:762-768
|
被引
3
次
|
|
|
|
|
16.
Liu W D. Novel insights into rice innate immunity against bacterial and fungal pathogens.
Annual Review of Phytopathology,2014,52:213-241
|
被引
24
次
|
|
|
|
|
17.
Mccouch S R. Mapping of blast resistance genes in rice.
Rice Blast Disease,1994:167
|
被引
9
次
|
|
|
|
|
18.
Qu S. The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice.
Genetics,2006,172:1901-1914
|
被引
107
次
|
|
|
|
|
19.
Ramkumar G. Nucleotide diversity of Pita a major blast resistance gene and identification of its minimal promoter.
Gene,2014,546:250-256
|
被引
2
次
|
|
|
|
|
20.
Saghai-Maroof M A. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics.
Proceedings of the National Academy of Sciences of the United States of America,1994,81:8014-8018
|
被引
488
次
|
|
|
|
|
了解气象卫星更多信息，请访问