麻欠精油对脂多糖诱导的THP-1细胞氧化应激的影响
Effect of Maqian (Zanthoxylum myriacanthum var. pubescens Huang) Essential Oil on Oxidative Stress in THP-1 Cells Stimulated by LPS
查看参考文献15篇
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文摘
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本研究主要探讨麻欠精油对脂多糖(LPS)诱导的THP-1细胞氧化应激的影响。溶剂对照、地塞米松或不同浓度的麻欠精油预处理THP-1细胞后,再用LPS培养24 h,用DCFH-DA荧光探针标记细胞后流式检测细胞内活性氧ROS;用FITC Annexin V/PI双染法流式检测细胞凋亡;用Griess试剂测定NO;用比色法检测总超氧化物歧化酶(SOD) ;用Western Blot法检测NADPH p47 phox的表达。结果发现麻欠精油处理组与溶剂对照组相比,LPS诱导的ROS和NO水平显著降低、SOD水平显著增加、NADPH p47 phox蛋白表达减低,细胞凋亡降低。以上实验结果表明麻欠精油对LPS诱导的THP-1细胞的氧化应激有明显抑制作用并对细胞凋亡有保护作用。 |
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其他语种文摘
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In this study, the effect of Maqian (Zanthoxylum myriacanthum var. pubescens Huang) essential oil (MQEO) on oxidative stress in lipopolysaccharide (LPS) -stimulated THP-1 cells was investigated. Cells were pretreated with vehicle, Dex or different doses of MQEO,and then cultured with LPS for 24 h. The intracellular reactive oxygen species (ROS) were determined by staining the cells with 2',7'-ichlorofluorescein diacetate (DCFH-DA) followed by flow cytometry analysis. Cells were stained with Annexin V-FITC/PI and assayed for apoptosis by flow cytometry. NO was determined by Griess reagent method. Cell lysates were prepared and assayed for the activity of intracellular superoxide dismutase (SOD) by a colorimetric method and the expression of p47 phox (the cytoplasmic subunit of NADPH oxidase) by western blotting. The results showed that, compared with cells treated with vehicles, cells treated with MQEO exhibited significantly reduced LPS stimulated ROS and NO production in a concentration-dependent manner. In addition,cells treated with MQEO showed significantly less LPS-induced decrease in SOD activity as well as the increase of expression of cytoplasmic subunit of NADPH oxidase (p47 phox) . Furthermore,cells treated MQEO exhibited markedly reduced LPS-stimulated apoptosis. Collectively, these findings suggested that MQEO had the ability to suppress oxidative stress in LPS-stimulated THP-1 cells and protect cells from apoptosis. MQEO had the potential application for the prevention and treatment of various diseases related to oxidative stress. |
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来源
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天然产物研究与开发
,2016,28(6):833-838 【核心库】
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DOI
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10.16333/j.1001-6880.2016.6.004
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关键词
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麻欠精油
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THP-1
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氧化应激
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细胞凋亡
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地址
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中国科学院研究生院, 中国科学院西双版纳热带植物园热带植物资源可持续利用重点实验室, 北京, 100049
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中国科学院西双版纳热带植物园热带植物资源可持续利用重点实验室, 中国科学院西双版纳热带植物园热带植物资源可持续利用重点实验室, 西双版纳, 666303
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中国科学院昆明植物研究所, 昆明, 650201
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1001-6880 |
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学科
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药学 |
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基金
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中国科学院“百人计划”项目
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文献收藏号
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CSCD:5753885
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