一种固定化黄嘌呤氧化酶的制备与性能评价
Preparation and Property Evaluation of a New Immobilized Xanthine Oxidase
查看参考文献23篇
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文摘
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为了使黄嘌呤氧化酶(XOD)可回收且能够长时间多次使用,该研究以色谱级硅球(SiO_2)为载体,采用共价键合的方式用碳纳米管(CNTs)对硅球进行包裹,并将XOD在碳纳米管包裹的硅胶球上进行固定,制备了一种以碳纳米管为介质的固定化黄嘌呤氧化酶(XOD@CNTs@SiO_2)。采用TEM和FTIR对固定化酶进行表面形貌和反应基团变化的表征;以产物尿酸为指标进行相对酶活性的评价;采用Bradford法对载体的担载量进行评价。结果表明,硅球表面经CNTs包裹后,XOD的担载量和相对酶活性分别提高2倍和2.6倍。XOD@CNTs@SiO_2在60 ℃储存时,储存时间至400 min后,活性仍能保持至最初的80%。4 ℃避光保存时,第11 d时相对活性仍为60%~70%。室温反应,连续使用至9次时,XOD@CNTs@SiO_2-30的相对酶活性仍能保留66.1%。 |
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其他语种文摘
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In order to make the xanthine oxidase(XOD)to be recoverable and durable,a new kind of immobilized XOD was prepared.The silica microspheres were chosen as the supporter and the carbon nanotubes(CNTs)were coated on the surface of microspheres by covalent bonding.Then,free XOD were immobilized on the surface of XOD@CNTs@SiO_2.The morphology and chemical group on the new XOD@CNTs@SiO_2 were characterized by TEM and FTIR,respectively.Uric acid,the reaction product,was used as the evaluation indicator of the relative activity.The loading of XOD was calculated by Bradford method.It was shown that,the loading and relative activities of XOD@CNTs@SiO_2 were increased 2 and 2.6 times than XOD@SiO_2.The relative activity could keep 80% after saving for 400 min under 60 ℃.The XOD@CNTs@SiO_2 were kept under 4 ℃ and away from light,and the relative activity could still keep 60%~70% until the 11th day.Under the normal temperature,the relative activity could keep 66.1% after 9 times continuous reactions. |
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来源
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精细化工
,2016,33(4):396-400 【核心库】
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DOI
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10.13550/j.jxhg.2016.04.007
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关键词
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黄嘌呤氧化酶
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碳纳米管
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固定化
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稳定性
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功能材料
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地址
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1.
中国科学院兰州化学物理研究所, 中国科学院西北特色植物资源化学重点实验室;;甘肃省天然药物重点实验室, 甘肃, 兰州, 730000
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中国科学院兰州化学物理研究所, 中国科学院西北特色植物资源化学重点实验室;;甘肃省天然药物重点实验室;;甘肃省高校中(藏)药化学与质量研究省级重点实验室, 甘肃, 兰州, 730000
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甘肃中医药大学药学系, 甘肃省高校中(藏)药化学与质量研究省级重点实验室, 甘肃, 兰州, 730000
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语种
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中文 |
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文献类型
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研究性论文 |
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ISSN
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1003-5214 |
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学科
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化学;化学工业 |
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基金
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国家高技术研究发展计划(863计划)
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国家自然科学基金
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文献收藏号
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CSCD:5687668
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