Effects of abiotic stress and hormones on the expressions of five 13-CmLOXs and enzyme activity in oriental melon (Cucumis melo var. makuwa Makino)
查看参考文献46篇
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文摘
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Lipoxygenases (LOXs) are a group of non-heme, iron-containing enzymes and extensively involved in plant growth and development, ripening and senescence, stress responses, biosynthesis of regulatory molecules and defense reaction. In our previous study, 18 LOXs in melon genome were screened and identified, and five 13-LOX genes (CmLOX08, CmLOX10, CmLOX12, CmLOX13 and CmLOX18) were predicted to locate in chloroplast. Phylogenetic analysis result showed that the five genes have high homology with jasmonic acid (JA) biosynthesis-related LOXs from other plants. In addition, promoter analysis revealed that motifs of the five genes participate in gene expression regulated by hormones and stresses. Therefore, we analyzed the expressions of the five genes and LOX activity in leaves of four-leaf stage seedlings of oriental melon cultivar Yumeiren under abiotic stress: wounding, cold, high temperature and hydrogen peroxide (H2O2), and signal molecule treatments: methyl jasmonate (MeJA), abscisic acid (ABA) and salicylic acid (SA). Real time qPCR revealed that wounding and H_2O_2 induced the expressions of all the five genes. Only CmLOX08 was induced by cold while only CmLOX13 was suppressed by high temperature. ABA induced the expressions of CmLOX10 and CmLOX12 while inhibited CmLOX13 and CmLOX18. MeJA increased the 3 genes expressions except CmLOX08 and CmLOX13, whereas SA decreased the effect, apart from CmLOX12. All the abiotic stresses and signal molecules treatments increased the LOX activity in leaves of oriental melon. In summary, the results suggest that the five genes have diverse functions in abiotic stress and hormone responses, and might participate in defense response. The data generated in this study will be helpful in subcellular localization and transgenic experiment to understand their precise roles in plant defense response. |
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来源
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Journal of Integrative Agriculture
,2016,15(2):326-338 【核心库】
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DOI
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10.1016/S2095-3119(15)61135-2
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关键词
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oriental melons
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lipoxygenase
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abiotic stress
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signal molecules
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gene expression
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地址
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College of Horticulture, Shenyang Agricultural University, Key Laboratory of Protected Horticulture of Liaoning Province;;Key Laboratory of Protected Horticulture, Ministry of Education, Shenyang, 110866
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语种
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英文 |
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文献类型
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研究性论文 |
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ISSN
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2095-3119 |
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学科
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园艺 |
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基金
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国家自然科学基金
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文献收藏号
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CSCD:5643758
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参考文献 共
46
共3页
|
|
1.
Andreou A. Lipoxygenases-structure and reaction mechanism.
Phytochemistry,2009,70:1504-1510
|
被引
14
次
|
|
|
|
|
2.
Axelrod B. Lipoxygenase from soybeans.
Methods in Enzymology,1981,71:441-451
|
被引
46
次
|
|
|
|
|
3.
Bate N J. C6-volatiles derived from the lipoxygenase pathway induce a subset of defense-related genes.
The Plant Journal,1998,16:561-569
|
被引
29
次
|
|
|
|
|
4.
Bell E. A chloroplast lipoxygenase is required for wound-induced jasmonic acid accumulation in Arabidopsis.
Proceedings of the National Academy of Sciences of the United States of America,1995,92:8675-8679
|
被引
39
次
|
|
|
|
|
5.
Bhardwaj P K. Lipoxygenase in Caragana jubata responds to low temperature, abscisic acid, methyl jasmonate and salicylic acid.
Gene,2011,483:49-53
|
被引
7
次
|
|
|
|
|
6.
Bradford M M. A rapid and sensitive method for the quantitation of micro-gram quantities of protein utilizing the principle of protein dye binding.
Analytical Biochemistry,1976,72:248-254
|
被引
7464
次
|
|
|
|
|
7.
Browse J. Jasmonate: An oxylipin signal with many roles in plants.
Vitamins and Hormones,2005,72:431-456
|
被引
18
次
|
|
|
|
|
8.
Cho K. Transgenic expression of dual positional maize lipoxygenase-1 leads to the regulation of defense-related signaling molecules and activation of the antioxidative enzyme system in rice.
Plant Science,2012,185:238-245
|
被引
1
次
|
|
|
|
|
9.
Christensen S A. The maize lipoxygenase, ZmLOX10, mediates green leaf volatile, jasmonate and herbivore-induced plant volatile production for defense against insect attack.
The Plant Journal,2013,74:59-73
|
被引
19
次
|
|
|
|
|
10.
Cipollini D. Salicylic acid inhibits jasmonic acid-induced resistance of Arabidopsis thaliana to Spodoptera exigua.
Molecular Ecology,2004,13:1643-1653
|
被引
15
次
|
|
|
|
|
11.
Demmig-Adams B. Emerging trade-offs - impact of photoprotectants (PsbS, xanthophylls, and vitamin E) on oxylipins as regulators of development and defense.
New Phytologist,2013,197:720-729
|
被引
3
次
|
|
|
|
|
12.
Dong X. SA, JA, ethylene, and disease resistance in plants.
Current Opinion in Plant Biology,1998,1:316-323
|
被引
38
次
|
|
|
|
|
13.
Engelberth J. Airborne signals prime plants against insect herbivore attack.
Proceedings of the National Academy of Sciences of the United States of America,2004,101:1781-1785
|
被引
49
次
|
|
|
|
|
14.
Engelberth J. Insect elicitors and exposure to green leafy volatiles differentially upregulate major octadecanoids and transcripts of 12-oxo phytodienoic acid reductases in Zea mays.
Molecular Plant-Microbe Interactions,2007,20:707-716
|
被引
8
次
|
|
|
|
|
15.
Feussner I. The lipoxygenase pathway.
Annual Review of Plant Biology,2002,53:275-297
|
被引
100
次
|
|
|
|
|
16.
Feys B J. Interplay of signaling pathways in plant disease resistance.
Trends in Genetics,2000,16:449-455
|
被引
38
次
|
|
|
|
|
17.
Garcia-Marcos A. Oxylipin biosynthesis genes positively regulate programmed cell death during compatible infections with the synergistic pair potato Virus X-Potato Virus Y and Tomato Spotted Wilt Virus.
Journal of Virology,2013,87:5769-5783
|
被引
4
次
|
|
|
|
|
18.
Glazebrook J. Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens.
Annual Reviews of Phytopathology,2005,43:205-227
|
被引
182
次
|
|
|
|
|
19.
Hall T A. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/ NT.
Nucleic Acids Symposium Series,1999,41:95-98
|
被引
774
次
|
|
|
|
|
20.
Halitschke R. Antisense LOX expression increases herbivore performance by decreasing defense response and inhibiting growth-related transcriptional reorganization in Nicotiana attenuata.
The Plant Journal,2003,36:794-807
|
被引
38
次
|
|
|
|
|
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