冷冻扫描电镜及其在生命科学研究中的应用
Cryo-scanning electron microscopy and its application in the research of life science
查看参考文献16篇
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文摘
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冷冻扫描电镜基于扫描电镜的超低温冷冻制样及传输技术发展而来,具有能在高真空状态下观察含水样品、分辨率高、制样简单快速、可对样品进行断裂刻蚀等优点,是生命科学研究的有力工具,尤其适合柔软样品的成像和监测冰冻环境对组织和细胞的影响。能否获得高质量的冷冻扫描电镜图像,取决于样品制备和观察条件是否合理和优化,要求操作人员能够快速高效地根据样品选择操作条件、捕捉快速变化的样品表面信息。 |
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其他语种文摘
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Cryo-scanning electron microscopy (Cryo-SEM),which combines the high-performance imaging of scanning electron microscopy (SEM) with cryogenic sample preparation techniques,allows scientists to investigate structures and materials in their native,hydrated state with image resolution now approaching 1 nm. Cryo-SEM is a powerful tool for characterizing the hydrated microstructures of biological specimens,especially for observing soft specimens (like tissues and cells) and their responses to freezing. Operator skill is the primary factor in determining the quality of the results. The operator's ability to quickly and efficiently scan the surface,manipulate conditions,and acquire images of the rapidly changing sample are very important. |
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来源
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电子显微学报
,2015,34(5):447-451 【核心库】
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DOI
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10.3969/j.issn.1000-6281.2015.05.016
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关键词
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冷冻扫描电镜
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含水样品
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低温冷冻制样
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地址
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中国科学院水生生物研究所, 湖北, 武汉, 430072
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语种
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中文 |
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文献类型
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综述型 |
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ISSN
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1000-6281 |
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学科
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遗传学 |
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文献收藏号
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CSCD:5541139
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参考文献 共
16
共1页
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